Experiment: Formate 100 mM hypophosphite
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08230 and MMP_RS08235 are separated by 106 nucleotides MMP_RS08235 and MMP_RS08240 are separated by 281 nucleotides MMP_RS08240 and MMP_RS08245 are separated by 24 nucleotides
MMP_RS08230: MMP_RS08230 - 50S ribosomal protein L11 methyltransferase, at 1,548,828 to 1,549,610
_RS08230
MMP_RS08235: MMP_RS08235 - hypothetical protein, at 1,549,717 to 1,549,944
_RS08235
MMP_RS08240: MMP_RS08240 - methanogenesis marker 16 metalloprotein, at 1,550,226 to 1,551,392
_RS08240
MMP_RS08245: MMP_RS08245 - DUF4013 domain-containing protein, at 1,551,417 to 1,552,286
_RS08245
Position (kb)
1550
1551
1552 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1549.287 kb on - strand, within MMP_RS08230 at 1549.303 kb on + strand, within MMP_RS08230 at 1549.333 kb on - strand, within MMP_RS08230 at 1549.351 kb on + strand, within MMP_RS08230 at 1549.369 kb on - strand, within MMP_RS08230 at 1549.370 kb on + strand, within MMP_RS08230 at 1549.398 kb on + strand, within MMP_RS08230 at 1549.538 kb on - strand at 1549.563 kb on - strand at 1549.576 kb on - strand at 1549.576 kb on - strand at 1549.577 kb on + strand at 1549.577 kb on + strand at 1549.656 kb on + strand at 1549.657 kb on - strand at 1549.657 kb on - strand at 1549.658 kb on + strand at 1549.658 kb on + strand at 1549.658 kb on + strand at 1549.658 kb on + strand at 1549.658 kb on + strand at 1549.714 kb on - strand at 1549.750 kb on + strand, within MMP_RS08235 at 1549.845 kb on - strand, within MMP_RS08235 at 1549.846 kb on + strand, within MMP_RS08235 at 1549.892 kb on - strand, within MMP_RS08235 at 1549.893 kb on + strand, within MMP_RS08235 at 1549.945 kb on + strand at 1549.975 kb on + strand at 1550.017 kb on + strand at 1550.062 kb on - strand at 1550.063 kb on + strand at 1550.082 kb on + strand at 1550.090 kb on + strand at 1550.123 kb on - strand at 1550.165 kb on - strand at 1550.171 kb on + strand at 1550.175 kb on + strand at 1550.237 kb on + strand at 1550.240 kb on + strand at 1550.257 kb on - strand at 1550.496 kb on - strand, within MMP_RS08240 at 1550.678 kb on + strand, within MMP_RS08240 at 1550.743 kb on - strand, within MMP_RS08240 at 1550.901 kb on - strand, within MMP_RS08240 at 1551.105 kb on + strand, within MMP_RS08240 at 1551.265 kb on - strand, within MMP_RS08240 at 1551.266 kb on + strand, within MMP_RS08240 at 1551.268 kb on + strand, within MMP_RS08240 at 1551.338 kb on + strand at 1551.357 kb on + strand at 1551.415 kb on + strand at 1551.609 kb on - strand, within MMP_RS08245 at 1551.610 kb on + strand, within MMP_RS08245 at 1551.610 kb on + strand, within MMP_RS08245 at 1551.682 kb on + strand, within MMP_RS08245 at 1551.769 kb on - strand, within MMP_RS08245 at 1551.770 kb on + strand, within MMP_RS08245 at 1551.770 kb on + strand, within MMP_RS08245 at 1551.864 kb on - strand, within MMP_RS08245 at 1551.865 kb on + strand, within MMP_RS08245 at 1551.894 kb on - strand, within MMP_RS08245 at 1552.036 kb on - strand, within MMP_RS08245 at 1552.107 kb on + strand, within MMP_RS08245 at 1552.271 kb on + strand at 1552.271 kb on + strand at 1552.314 kb on + strand at 1552.328 kb on + strand at 1552.329 kb on - strand at 1552.329 kb on - strand at 1552.329 kb on - strand at 1552.329 kb on - strand at 1552.330 kb on + strand at 1552.330 kb on + strand at 1552.333 kb on - strand at 1552.361 kb on - strand at 1552.361 kb on - strand at 1552.361 kb on - strand at 1552.362 kb on + strand at 1552.362 kb on + strand at 1552.362 kb on + strand at 1552.365 kb on - strand at 1552.365 kb on - strand at 1552.369 kb on - strand at 1552.380 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate 100 mM hypophosphite remove 1,549,287 - MMP_RS08230 0.59 +0.4 1,549,303 + MMP_RS08230 0.61 +0.9 1,549,333 - MMP_RS08230 0.64 +0.5 1,549,351 + MMP_RS08230 0.67 +0.6 1,549,369 - MMP_RS08230 0.69 +2.4 1,549,370 + MMP_RS08230 0.69 +0.5 1,549,398 + MMP_RS08230 0.73 +0.0 1,549,538 - +0.8 1,549,563 - +0.6 1,549,576 - +0.5 1,549,576 - +0.3 1,549,577 + +0.7 1,549,577 + +0.3 1,549,656 + -0.4 1,549,657 - +0.4 1,549,657 - +0.4 1,549,658 + +0.7 1,549,658 + +0.7 1,549,658 + +0.5 1,549,658 + -0.2 1,549,658 + +0.9 1,549,714 - +0.3 1,549,750 + MMP_RS08235 0.14 +0.3 1,549,845 - MMP_RS08235 0.56 +0.1 1,549,846 + MMP_RS08235 0.57 -0.7 1,549,892 - MMP_RS08235 0.77 +0.6 1,549,893 + MMP_RS08235 0.77 +0.0 1,549,945 + +0.6 1,549,975 + +0.3 1,550,017 + +0.6 1,550,062 - +0.3 1,550,063 + +1.1 1,550,082 + -0.8 1,550,090 + -0.2 1,550,123 - +0.3 1,550,165 - +1.2 1,550,171 + +0.2 1,550,175 + +0.7 1,550,237 + +2.0 1,550,240 + +2.6 1,550,257 - +3.1 1,550,496 - MMP_RS08240 0.23 +2.8 1,550,678 + MMP_RS08240 0.39 +2.0 1,550,743 - MMP_RS08240 0.44 +0.8 1,550,901 - MMP_RS08240 0.58 +1.7 1,551,105 + MMP_RS08240 0.75 +2.0 1,551,265 - MMP_RS08240 0.89 +2.1 1,551,266 + MMP_RS08240 0.89 +1.6 1,551,268 + MMP_RS08240 0.89 +2.5 1,551,338 + +1.1 1,551,357 + +2.2 1,551,415 + -0.2 1,551,609 - MMP_RS08245 0.22 +0.8 1,551,610 + MMP_RS08245 0.22 +0.6 1,551,610 + MMP_RS08245 0.22 +0.6 1,551,682 + MMP_RS08245 0.30 -0.1 1,551,769 - MMP_RS08245 0.40 +0.4 1,551,770 + MMP_RS08245 0.41 +0.6 1,551,770 + MMP_RS08245 0.41 +0.8 1,551,864 - MMP_RS08245 0.51 -0.2 1,551,865 + MMP_RS08245 0.51 +0.5 1,551,894 - MMP_RS08245 0.55 +0.2 1,552,036 - MMP_RS08245 0.71 +0.4 1,552,107 + MMP_RS08245 0.79 -0.1 1,552,271 + +0.2 1,552,271 + +0.7 1,552,314 + -0.5 1,552,328 + +0.2 1,552,329 - +0.1 1,552,329 - -0.0 1,552,329 - -0.3 1,552,329 - +0.3 1,552,330 + +0.6 1,552,330 + +0.1 1,552,333 - +0.3 1,552,361 - +0.5 1,552,361 - -0.1 1,552,361 - +0.5 1,552,362 + -0.7 1,552,362 + +1.0 1,552,362 + -0.0 1,552,365 - +0.2 1,552,365 - +0.1 1,552,369 - +0.7 1,552,380 + +0.1
Or see this region's nucleotide sequence