Experiment: Formate 100 mM hypophosphite
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01725 and MMP_RS01730 are separated by 50 nucleotides MMP_RS01730 and MMP_RS01735 are separated by 66 nucleotides MMP_RS01735 and MMP_RS01740 are separated by 206 nucleotides
MMP_RS01725: MMP_RS01725 - class I SAM-dependent methyltransferase family protein, at 319,643 to 320,671
_RS01725
MMP_RS01730: MMP_RS01730 - hypothetical protein, at 320,722 to 321,102
_RS01730
MMP_RS01735: MMP_RS01735 - type II glyceraldehyde-3-phosphate dehydrogenase, at 321,169 to 322,191
_RS01735
MMP_RS01740: MMP_RS01740 - methionine--tRNA ligase, at 322,398 to 324,389
_RS01740
Position (kb) 321
322
323 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 320.173 kb on + strand, within MMP_RS01725 at 320.432 kb on + strand, within MMP_RS01725 at 320.584 kb on - strand at 320.594 kb on + strand at 320.650 kb on - strand at 320.695 kb on + strand at 320.696 kb on - strand at 320.697 kb on + strand at 320.698 kb on - strand at 320.698 kb on - strand at 320.709 kb on - strand at 320.734 kb on + strand at 320.743 kb on + strand at 320.844 kb on - strand at 321.020 kb on + strand at 321.023 kb on - strand at 321.024 kb on + strand at 321.308 kb on + strand at 321.319 kb on - strand at 321.951 kb on - strand at 321.952 kb on + strand at 322.226 kb on - strand at 322.258 kb on - strand at 322.262 kb on - strand at 322.265 kb on + strand at 322.271 kb on - strand at 322.272 kb on + strand at 322.275 kb on - strand at 322.276 kb on + strand at 322.277 kb on - strand at 322.280 kb on - strand at 322.285 kb on - strand at 322.286 kb on + strand at 322.287 kb on - strand at 322.288 kb on + strand at 322.327 kb on + strand at 322.328 kb on - strand at 322.409 kb on + strand at 322.413 kb on + strand at 322.428 kb on - strand at 322.920 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate 100 mM hypophosphite remove 320,173 + MMP_RS01725 0.52 -2.5 320,432 + MMP_RS01725 0.77 -2.9 320,584 - -2.6 320,594 + -1.8 320,650 - -0.7 320,695 + -0.1 320,696 - +0.2 320,697 + -0.5 320,698 - +0.2 320,698 - -0.4 320,709 - -0.3 320,734 + -0.0 320,743 + +0.4 320,844 - -2.1 321,020 + -2.1 321,023 - -1.6 321,024 + -2.7 321,308 + -0.5 321,319 - -0.4 321,951 - -2.2 321,952 + -1.5 322,226 - +0.0 322,258 - +1.3 322,262 - -0.3 322,265 + -1.4 322,271 - +2.5 322,272 + -0.7 322,275 - +2.8 322,276 + +3.3 322,277 - +2.5 322,280 - +2.6 322,285 - +1.9 322,286 + +2.1 322,287 - +1.9 322,288 + +1.5 322,327 + -1.9 322,328 - -2.8 322,409 + -1.1 322,413 + -0.9 322,428 - -1.4 322,920 + -1.1
Or see this region's nucleotide sequence