Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS22775 and RALBFv3_RS24770 are separated by 114 nucleotides RALBFv3_RS24770 and RALBFv3_RS22780 are separated by 14 nucleotides RALBFv3_RS22780 and RALBFv3_RS24775 are separated by 7 nucleotides RALBFv3_RS24775 and RALBFv3_RS22785 overlap by 22 nucleotides
RALBFv3_RS22775: RALBFv3_RS22775 - hypothetical protein, at 1,882,041 to 1,882,694
_RS22775
RALBFv3_RS24770: RALBFv3_RS24770 - hypothetical protein, at 1,882,809 to 1,883,123
_RS24770
RALBFv3_RS22780: RALBFv3_RS22780 - metallophosphoesterase, at 1,883,138 to 1,883,878
_RS22780
RALBFv3_RS24775: RALBFv3_RS24775 - hypothetical protein, at 1,883,886 to 1,884,092
_RS24775
RALBFv3_RS22785: RALBFv3_RS22785 - hypothetical protein, at 1,884,071 to 1,884,442
_RS22785
Position (kb)
1882
1883
1884 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1881.913 kb on + strand at 1881.914 kb on - strand at 1881.914 kb on - strand at 1881.914 kb on - strand at 1881.914 kb on - strand at 1881.914 kb on - strand at 1881.959 kb on + strand at 1881.959 kb on + strand at 1882.012 kb on - strand at 1882.061 kb on + strand at 1882.061 kb on + strand at 1882.061 kb on + strand at 1882.062 kb on - strand at 1882.062 kb on - strand at 1882.062 kb on - strand at 1882.068 kb on + strand at 1882.068 kb on + strand at 1882.069 kb on - strand at 1882.069 kb on - strand at 1882.094 kb on + strand at 1882.095 kb on - strand at 1882.100 kb on - strand at 1882.176 kb on - strand, within RALBFv3_RS22775 at 1882.176 kb on - strand, within RALBFv3_RS22775 at 1882.202 kb on + strand, within RALBFv3_RS22775 at 1882.203 kb on - strand, within RALBFv3_RS22775 at 1882.203 kb on - strand, within RALBFv3_RS22775 at 1882.204 kb on + strand, within RALBFv3_RS22775 at 1882.204 kb on + strand, within RALBFv3_RS22775 at 1882.204 kb on + strand, within RALBFv3_RS22775 at 1882.205 kb on - strand, within RALBFv3_RS22775 at 1882.205 kb on - strand, within RALBFv3_RS22775 at 1882.205 kb on - strand, within RALBFv3_RS22775 at 1882.213 kb on + strand, within RALBFv3_RS22775 at 1882.214 kb on - strand, within RALBFv3_RS22775 at 1882.214 kb on - strand, within RALBFv3_RS22775 at 1882.217 kb on + strand, within RALBFv3_RS22775 at 1882.218 kb on - strand, within RALBFv3_RS22775 at 1882.218 kb on - strand, within RALBFv3_RS22775 at 1882.218 kb on - strand, within RALBFv3_RS22775 at 1882.218 kb on - strand, within RALBFv3_RS22775 at 1882.249 kb on + strand, within RALBFv3_RS22775 at 1882.249 kb on + strand, within RALBFv3_RS22775 at 1882.250 kb on - strand, within RALBFv3_RS22775 at 1882.250 kb on - strand, within RALBFv3_RS22775 at 1882.279 kb on + strand, within RALBFv3_RS22775 at 1882.280 kb on - strand, within RALBFv3_RS22775 at 1882.280 kb on - strand, within RALBFv3_RS22775 at 1882.280 kb on - strand, within RALBFv3_RS22775 at 1882.290 kb on - strand, within RALBFv3_RS22775 at 1882.490 kb on + strand, within RALBFv3_RS22775 at 1882.491 kb on - strand, within RALBFv3_RS22775 at 1882.491 kb on - strand, within RALBFv3_RS22775 at 1882.497 kb on + strand, within RALBFv3_RS22775 at 1882.498 kb on - strand, within RALBFv3_RS22775 at 1882.498 kb on - strand, within RALBFv3_RS22775 at 1882.611 kb on + strand, within RALBFv3_RS22775 at 1882.611 kb on + strand, within RALBFv3_RS22775 at 1882.611 kb on + strand, within RALBFv3_RS22775 at 1882.611 kb on + strand, within RALBFv3_RS22775 at 1882.612 kb on - strand, within RALBFv3_RS22775 at 1882.612 kb on - strand, within RALBFv3_RS22775 at 1882.612 kb on - strand, within RALBFv3_RS22775 at 1882.620 kb on - strand, within RALBFv3_RS22775 at 1882.722 kb on - strand at 1882.725 kb on + strand at 1882.725 kb on + strand at 1882.728 kb on + strand at 1882.729 kb on - strand at 1882.729 kb on - strand at 1882.730 kb on + strand at 1882.730 kb on + strand at 1882.738 kb on - strand at 1882.799 kb on + strand at 1882.800 kb on - strand at 1882.800 kb on - strand at 1882.800 kb on - strand at 1882.900 kb on + strand, within RALBFv3_RS24770 at 1882.900 kb on + strand, within RALBFv3_RS24770 at 1882.901 kb on - strand, within RALBFv3_RS24770 at 1882.932 kb on + strand, within RALBFv3_RS24770 at 1882.932 kb on + strand, within RALBFv3_RS24770 at 1882.933 kb on - strand, within RALBFv3_RS24770 at 1882.933 kb on - strand, within RALBFv3_RS24770 at 1882.960 kb on - strand, within RALBFv3_RS24770 at 1883.022 kb on + strand, within RALBFv3_RS24770 at 1883.023 kb on - strand, within RALBFv3_RS24770 at 1883.023 kb on - strand, within RALBFv3_RS24770 at 1883.039 kb on - strand, within RALBFv3_RS24770 at 1883.039 kb on - strand, within RALBFv3_RS24770 at 1883.039 kb on - strand, within RALBFv3_RS24770 at 1883.039 kb on - strand, within RALBFv3_RS24770 at 1883.289 kb on + strand, within RALBFv3_RS22780 at 1883.378 kb on + strand, within RALBFv3_RS22780 at 1883.378 kb on + strand, within RALBFv3_RS22780 at 1883.379 kb on - strand, within RALBFv3_RS22780 at 1883.441 kb on + strand, within RALBFv3_RS22780 at 1883.441 kb on + strand, within RALBFv3_RS22780 at 1883.441 kb on + strand, within RALBFv3_RS22780 at 1883.441 kb on + strand, within RALBFv3_RS22780 at 1883.441 kb on + strand, within RALBFv3_RS22780 at 1883.442 kb on - strand, within RALBFv3_RS22780 at 1883.442 kb on - strand, within RALBFv3_RS22780 at 1883.495 kb on + strand, within RALBFv3_RS22780 at 1883.495 kb on + strand, within RALBFv3_RS22780 at 1883.495 kb on + strand, within RALBFv3_RS22780 at 1883.496 kb on - strand, within RALBFv3_RS22780 at 1883.496 kb on - strand, within RALBFv3_RS22780 at 1883.641 kb on + strand, within RALBFv3_RS22780 at 1883.642 kb on - strand, within RALBFv3_RS22780 at 1883.675 kb on + strand, within RALBFv3_RS22780 at 1883.675 kb on + strand, within RALBFv3_RS22780 at 1883.676 kb on - strand, within RALBFv3_RS22780 at 1883.676 kb on - strand, within RALBFv3_RS22780 at 1883.733 kb on - strand, within RALBFv3_RS22780 at 1883.733 kb on - strand, within RALBFv3_RS22780 at 1883.789 kb on + strand, within RALBFv3_RS22780 at 1883.789 kb on + strand, within RALBFv3_RS22780 at 1884.019 kb on - strand, within RALBFv3_RS24775 at 1884.019 kb on - strand, within RALBFv3_RS24775 at 1884.019 kb on - strand, within RALBFv3_RS24775 at 1884.019 kb on - strand, within RALBFv3_RS24775 at 1884.019 kb on - strand, within RALBFv3_RS24775 at 1884.110 kb on + strand, within RALBFv3_RS22785 at 1884.110 kb on + strand, within RALBFv3_RS22785
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 1,881,913 + -0.3 1,881,914 - -0.2 1,881,914 - -0.8 1,881,914 - +0.1 1,881,914 - -1.6 1,881,914 - -0.3 1,881,959 + -1.8 1,881,959 + -0.5 1,882,012 - -0.5 1,882,061 + +0.1 1,882,061 + -0.9 1,882,061 + -0.5 1,882,062 - -0.8 1,882,062 - -0.4 1,882,062 - -0.4 1,882,068 + -0.1 1,882,068 + -0.9 1,882,069 - -0.0 1,882,069 - +0.4 1,882,094 + -1.9 1,882,095 - -0.4 1,882,100 - -0.2 1,882,176 - RALBFv3_RS22775 0.21 -2.0 1,882,176 - RALBFv3_RS22775 0.21 +0.3 1,882,202 + RALBFv3_RS22775 0.25 -2.7 1,882,203 - RALBFv3_RS22775 0.25 +0.8 1,882,203 - RALBFv3_RS22775 0.25 -0.9 1,882,204 + RALBFv3_RS22775 0.25 -2.0 1,882,204 + RALBFv3_RS22775 0.25 -0.9 1,882,204 + RALBFv3_RS22775 0.25 -1.8 1,882,205 - RALBFv3_RS22775 0.25 -0.4 1,882,205 - RALBFv3_RS22775 0.25 -0.9 1,882,205 - RALBFv3_RS22775 0.25 +0.1 1,882,213 + RALBFv3_RS22775 0.26 -1.4 1,882,214 - RALBFv3_RS22775 0.26 +0.8 1,882,214 - RALBFv3_RS22775 0.26 -0.8 1,882,217 + RALBFv3_RS22775 0.27 -0.9 1,882,218 - RALBFv3_RS22775 0.27 -0.6 1,882,218 - RALBFv3_RS22775 0.27 -0.8 1,882,218 - RALBFv3_RS22775 0.27 -0.5 1,882,218 - RALBFv3_RS22775 0.27 -0.6 1,882,249 + RALBFv3_RS22775 0.32 -1.2 1,882,249 + RALBFv3_RS22775 0.32 -0.2 1,882,250 - RALBFv3_RS22775 0.32 -0.3 1,882,250 - RALBFv3_RS22775 0.32 +0.8 1,882,279 + RALBFv3_RS22775 0.36 -0.9 1,882,280 - RALBFv3_RS22775 0.37 +0.8 1,882,280 - RALBFv3_RS22775 0.37 -1.7 1,882,280 - RALBFv3_RS22775 0.37 +0.3 1,882,290 - RALBFv3_RS22775 0.38 -0.5 1,882,490 + RALBFv3_RS22775 0.69 -1.2 1,882,491 - RALBFv3_RS22775 0.69 -0.6 1,882,491 - RALBFv3_RS22775 0.69 -0.8 1,882,497 + RALBFv3_RS22775 0.70 -2.3 1,882,498 - RALBFv3_RS22775 0.70 -1.4 1,882,498 - RALBFv3_RS22775 0.70 -1.2 1,882,611 + RALBFv3_RS22775 0.87 -1.8 1,882,611 + RALBFv3_RS22775 0.87 -2.0 1,882,611 + RALBFv3_RS22775 0.87 -2.3 1,882,611 + RALBFv3_RS22775 0.87 -0.6 1,882,612 - RALBFv3_RS22775 0.87 +0.8 1,882,612 - RALBFv3_RS22775 0.87 -0.3 1,882,612 - RALBFv3_RS22775 0.87 -0.5 1,882,620 - RALBFv3_RS22775 0.89 -0.9 1,882,722 - -0.0 1,882,725 + -1.2 1,882,725 + -2.0 1,882,728 + -2.1 1,882,729 - -1.4 1,882,729 - -0.8 1,882,730 + -1.5 1,882,730 + -1.8 1,882,738 - +1.0 1,882,799 + -1.8 1,882,800 - -0.1 1,882,800 - -0.6 1,882,800 - +0.3 1,882,900 + RALBFv3_RS24770 0.29 -1.4 1,882,900 + RALBFv3_RS24770 0.29 -0.8 1,882,901 - RALBFv3_RS24770 0.29 +0.5 1,882,932 + RALBFv3_RS24770 0.39 -2.9 1,882,932 + RALBFv3_RS24770 0.39 -2.0 1,882,933 - RALBFv3_RS24770 0.39 -0.3 1,882,933 - RALBFv3_RS24770 0.39 +0.8 1,882,960 - RALBFv3_RS24770 0.48 -0.9 1,883,022 + RALBFv3_RS24770 0.68 -1.9 1,883,023 - RALBFv3_RS24770 0.68 +0.3 1,883,023 - RALBFv3_RS24770 0.68 -0.5 1,883,039 - RALBFv3_RS24770 0.73 +0.5 1,883,039 - RALBFv3_RS24770 0.73 -0.3 1,883,039 - RALBFv3_RS24770 0.73 +2.4 1,883,039 - RALBFv3_RS24770 0.73 +0.8 1,883,289 + RALBFv3_RS22780 0.20 -1.1 1,883,378 + RALBFv3_RS22780 0.32 -1.6 1,883,378 + RALBFv3_RS22780 0.32 -1.5 1,883,379 - RALBFv3_RS22780 0.33 -0.7 1,883,441 + RALBFv3_RS22780 0.41 -2.5 1,883,441 + RALBFv3_RS22780 0.41 -1.5 1,883,441 + RALBFv3_RS22780 0.41 -0.7 1,883,441 + RALBFv3_RS22780 0.41 -3.0 1,883,441 + RALBFv3_RS22780 0.41 -1.6 1,883,442 - RALBFv3_RS22780 0.41 -0.4 1,883,442 - RALBFv3_RS22780 0.41 +0.0 1,883,495 + RALBFv3_RS22780 0.48 -1.7 1,883,495 + RALBFv3_RS22780 0.48 +0.8 1,883,495 + RALBFv3_RS22780 0.48 -2.4 1,883,496 - RALBFv3_RS22780 0.48 +0.8 1,883,496 - RALBFv3_RS22780 0.48 -0.0 1,883,641 + RALBFv3_RS22780 0.68 -2.0 1,883,642 - RALBFv3_RS22780 0.68 -1.8 1,883,675 + RALBFv3_RS22780 0.72 -1.5 1,883,675 + RALBFv3_RS22780 0.72 -1.8 1,883,676 - RALBFv3_RS22780 0.73 -0.7 1,883,676 - RALBFv3_RS22780 0.73 +0.6 1,883,733 - RALBFv3_RS22780 0.80 -0.3 1,883,733 - RALBFv3_RS22780 0.80 +0.8 1,883,789 + RALBFv3_RS22780 0.88 -1.0 1,883,789 + RALBFv3_RS22780 0.88 -3.5 1,884,019 - RALBFv3_RS24775 0.64 +0.3 1,884,019 - RALBFv3_RS24775 0.64 -0.7 1,884,019 - RALBFv3_RS24775 0.64 -0.2 1,884,019 - RALBFv3_RS24775 0.64 +0.2 1,884,019 - RALBFv3_RS24775 0.64 -0.8 1,884,110 + RALBFv3_RS22785 0.10 -3.5 1,884,110 + RALBFv3_RS22785 0.10 -3.7
Or see this region's nucleotide sequence