Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS21370 and RALBFv3_RS24610 overlap by 4 nucleotides RALBFv3_RS24610 and RALBFv3_RS21380 are separated by 388 nucleotides
RALBFv3_RS21370: RALBFv3_RS21370 - IS21 family transposase, at 1,517,816 to 1,518,832
_RS21370
RALBFv3_RS24610: RALBFv3_RS24610 - AAA family ATPase, at 1,518,829 to 1,519,095
_RS24610
RALBFv3_RS21380: RALBFv3_RS21380 - hypothetical protein, at 1,519,484 to 1,519,957
_RS21380
Position (kb)
1518
1519
1520 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1519.054 kb on + strand, within RALBFv3_RS24610 at 1519.115 kb on - strand at 1519.185 kb on + strand at 1519.185 kb on + strand at 1519.185 kb on + strand at 1519.186 kb on - strand at 1519.307 kb on + strand at 1519.308 kb on - strand at 1519.308 kb on - strand at 1519.308 kb on - strand at 1519.386 kb on + strand at 1519.421 kb on + strand at 1519.462 kb on + strand at 1519.462 kb on + strand at 1519.463 kb on - strand at 1519.463 kb on - strand at 1519.463 kb on - strand at 1519.463 kb on - strand at 1519.463 kb on - strand at 1519.592 kb on + strand, within RALBFv3_RS21380 at 1519.593 kb on - strand, within RALBFv3_RS21380 at 1519.593 kb on - strand, within RALBFv3_RS21380 at 1519.615 kb on + strand, within RALBFv3_RS21380 at 1519.616 kb on - strand, within RALBFv3_RS21380 at 1519.690 kb on + strand, within RALBFv3_RS21380 at 1519.690 kb on + strand, within RALBFv3_RS21380 at 1519.690 kb on + strand, within RALBFv3_RS21380 at 1519.691 kb on - strand, within RALBFv3_RS21380 at 1519.691 kb on - strand, within RALBFv3_RS21380 at 1519.723 kb on + strand, within RALBFv3_RS21380 at 1519.723 kb on + strand, within RALBFv3_RS21380 at 1519.723 kb on + strand, within RALBFv3_RS21380 at 1519.724 kb on - strand, within RALBFv3_RS21380 at 1519.724 kb on - strand, within RALBFv3_RS21380 at 1519.724 kb on - strand, within RALBFv3_RS21380 at 1519.724 kb on - strand, within RALBFv3_RS21380 at 1519.898 kb on + strand, within RALBFv3_RS21380 at 1519.898 kb on + strand, within RALBFv3_RS21380 at 1519.922 kb on + strand at 1519.923 kb on - strand at 1519.923 kb on - strand at 1519.923 kb on - strand at 1519.923 kb on - strand at 1519.923 kb on - strand at 1520.079 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 1,519,054 + RALBFv3_RS24610 0.84 -0.7 1,519,115 - +0.4 1,519,185 + -0.2 1,519,185 + -0.4 1,519,185 + -0.0 1,519,186 - +0.1 1,519,307 + -0.2 1,519,308 - -0.4 1,519,308 - +0.4 1,519,308 - +0.5 1,519,386 + +0.2 1,519,421 + +0.2 1,519,462 + -2.7 1,519,462 + -0.7 1,519,463 - -1.1 1,519,463 - +0.3 1,519,463 - -0.4 1,519,463 - -0.1 1,519,463 - +0.1 1,519,592 + RALBFv3_RS21380 0.23 +0.3 1,519,593 - RALBFv3_RS21380 0.23 +0.1 1,519,593 - RALBFv3_RS21380 0.23 -0.4 1,519,615 + RALBFv3_RS21380 0.28 -0.1 1,519,616 - RALBFv3_RS21380 0.28 -1.3 1,519,690 + RALBFv3_RS21380 0.43 +0.6 1,519,690 + RALBFv3_RS21380 0.43 -0.2 1,519,690 + RALBFv3_RS21380 0.43 +0.0 1,519,691 - RALBFv3_RS21380 0.44 +0.1 1,519,691 - RALBFv3_RS21380 0.44 +0.4 1,519,723 + RALBFv3_RS21380 0.50 -0.5 1,519,723 + RALBFv3_RS21380 0.50 -0.4 1,519,723 + RALBFv3_RS21380 0.50 +1.8 1,519,724 - RALBFv3_RS21380 0.51 -0.1 1,519,724 - RALBFv3_RS21380 0.51 -0.8 1,519,724 - RALBFv3_RS21380 0.51 -0.2 1,519,724 - RALBFv3_RS21380 0.51 -1.5 1,519,898 + RALBFv3_RS21380 0.87 +0.1 1,519,898 + RALBFv3_RS21380 0.87 -0.1 1,519,922 + -0.8 1,519,923 - -1.0 1,519,923 - -0.4 1,519,923 - +0.8 1,519,923 - -0.6 1,519,923 - +0.5 1,520,079 + -0.3
Or see this region's nucleotide sequence