Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS24515

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS20685 and RALBFv3_RS24515 are separated by 456 nucleotidesRALBFv3_RS24515 and RALBFv3_RS20690 overlap by 61 nucleotides RALBFv3_RS20685: RALBFv3_RS20685 - TetR family transcriptional regulator, at 1,337,384 to 1,338,028 _RS20685 RALBFv3_RS24515: RALBFv3_RS24515 - hypothetical protein, at 1,338,485 to 1,338,694 _RS24515 RALBFv3_RS20690: RALBFv3_RS20690 - chemotaxis protein CheV, at 1,338,634 to 1,339,578 _RS20690 Position (kb) 1338 1339Strain fitness (log2 ratio) -1 0 1 2 3at 1337.709 kb on - strand, within RALBFv3_RS20685at 1337.900 kb on + strand, within RALBFv3_RS20685at 1337.901 kb on - strand, within RALBFv3_RS20685at 1338.026 kb on + strandat 1338.026 kb on + strandat 1338.026 kb on + strandat 1338.026 kb on + strandat 1338.026 kb on + strandat 1338.027 kb on - strandat 1338.027 kb on - strandat 1338.027 kb on - strandat 1338.027 kb on - strandat 1338.027 kb on - strandat 1338.527 kb on + strand, within RALBFv3_RS24515at 1338.528 kb on - strand, within RALBFv3_RS24515at 1338.528 kb on - strand, within RALBFv3_RS24515at 1338.528 kb on - strand, within RALBFv3_RS24515at 1338.528 kb on - strand, within RALBFv3_RS24515at 1339.287 kb on - strand, within RALBFv3_RS20690at 1339.605 kb on + strandat 1339.647 kb on + strandat 1339.647 kb on + strandat 1339.647 kb on + strandat 1339.648 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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1,337,709 - RALBFv3_RS20685 0.50 -0.2
1,337,900 + RALBFv3_RS20685 0.80 -0.4
1,337,901 - RALBFv3_RS20685 0.80 -0.1
1,338,026 + -0.6
1,338,026 + +0.0
1,338,026 + -0.1
1,338,026 + -0.2
1,338,026 + -0.2
1,338,027 - -0.4
1,338,027 - -0.1
1,338,027 - -0.6
1,338,027 - -0.3
1,338,027 - +0.1
1,338,527 + RALBFv3_RS24515 0.20 -1.5
1,338,528 - RALBFv3_RS24515 0.20 +2.8
1,338,528 - RALBFv3_RS24515 0.20 +0.8
1,338,528 - RALBFv3_RS24515 0.20 -0.2
1,338,528 - RALBFv3_RS24515 0.20 -1.5
1,339,287 - RALBFv3_RS20690 0.69 +0.0
1,339,605 + -0.4
1,339,647 + +0.1
1,339,647 + +0.7
1,339,647 + -0.4
1,339,648 - -0.2

Or see this region's nucleotide sequence