Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS17950 and RALBFv3_RS24305 are separated by 568 nucleotides RALBFv3_RS24305 and RALBFv3_RS17955 are separated by 549 nucleotides
RALBFv3_RS17950: RALBFv3_RS17950 - hypothetical protein, at 531,413 to 532,864
_RS17950
RALBFv3_RS24305: RALBFv3_RS24305 - hypothetical protein, at 533,433 to 533,636
_RS24305
RALBFv3_RS17955: RALBFv3_RS17955 - alpha/beta hydrolase, at 534,186 to 535,004
_RS17955
Position (kb)
533
534 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 532.485 kb on + strand, within RALBFv3_RS17950 at 532.486 kb on - strand, within RALBFv3_RS17950 at 532.486 kb on - strand, within RALBFv3_RS17950 at 532.486 kb on - strand, within RALBFv3_RS17950 at 532.486 kb on - strand, within RALBFv3_RS17950 at 532.486 kb on - strand, within RALBFv3_RS17950 at 532.505 kb on - strand, within RALBFv3_RS17950 at 532.570 kb on + strand, within RALBFv3_RS17950 at 532.570 kb on + strand, within RALBFv3_RS17950 at 532.571 kb on - strand, within RALBFv3_RS17950 at 532.571 kb on - strand, within RALBFv3_RS17950 at 532.634 kb on + strand, within RALBFv3_RS17950 at 532.658 kb on + strand, within RALBFv3_RS17950 at 532.675 kb on - strand, within RALBFv3_RS17950 at 532.675 kb on - strand, within RALBFv3_RS17950 at 532.675 kb on - strand, within RALBFv3_RS17950 at 532.724 kb on + strand at 532.728 kb on + strand at 532.728 kb on + strand at 532.728 kb on + strand at 532.728 kb on + strand at 532.729 kb on - strand at 532.729 kb on - strand at 532.729 kb on - strand at 532.868 kb on + strand at 532.868 kb on + strand at 532.904 kb on + strand at 532.904 kb on + strand at 532.904 kb on + strand at 532.904 kb on + strand at 532.905 kb on - strand at 532.905 kb on - strand at 532.905 kb on - strand at 533.047 kb on - strand at 533.181 kb on - strand at 533.279 kb on + strand at 533.611 kb on - strand, within RALBFv3_RS24305 at 533.678 kb on + strand at 533.678 kb on + strand at 533.679 kb on - strand at 533.783 kb on + strand at 533.783 kb on + strand at 533.783 kb on + strand at 533.784 kb on - strand at 533.784 kb on - strand at 533.784 kb on - strand at 533.784 kb on - strand at 533.784 kb on - strand at 533.785 kb on + strand at 533.785 kb on + strand at 533.785 kb on + strand at 533.786 kb on - strand at 533.885 kb on + strand at 533.885 kb on + strand at 533.885 kb on + strand at 533.885 kb on + strand at 533.885 kb on + strand at 533.885 kb on + strand at 533.886 kb on - strand at 533.886 kb on - strand at 533.886 kb on - strand at 533.905 kb on + strand at 533.905 kb on + strand at 533.906 kb on - strand at 533.906 kb on - strand at 533.906 kb on - strand at 533.942 kb on - strand at 533.942 kb on - strand at 533.955 kb on + strand at 533.955 kb on + strand at 533.955 kb on + strand at 533.955 kb on + strand at 533.956 kb on - strand at 533.956 kb on - strand at 533.956 kb on - strand at 533.956 kb on - strand at 534.250 kb on + strand at 534.250 kb on + strand at 534.250 kb on + strand at 534.251 kb on - strand at 534.251 kb on - strand at 534.251 kb on - strand at 534.251 kb on - strand at 534.251 kb on - strand at 534.251 kb on - strand at 534.251 kb on - strand at 534.319 kb on + strand, within RALBFv3_RS17955 at 534.346 kb on + strand, within RALBFv3_RS17955 at 534.346 kb on + strand, within RALBFv3_RS17955 at 534.346 kb on + strand, within RALBFv3_RS17955 at 534.346 kb on + strand, within RALBFv3_RS17955 at 534.346 kb on + strand, within RALBFv3_RS17955 at 534.346 kb on + strand, within RALBFv3_RS17955 at 534.346 kb on + strand, within RALBFv3_RS17955 at 534.347 kb on - strand, within RALBFv3_RS17955 at 534.347 kb on - strand, within RALBFv3_RS17955 at 534.347 kb on - strand, within RALBFv3_RS17955 at 534.347 kb on - strand, within RALBFv3_RS17955 at 534.347 kb on - strand, within RALBFv3_RS17955 at 534.347 kb on - strand, within RALBFv3_RS17955 at 534.347 kb on - strand, within RALBFv3_RS17955 at 534.347 kb on - strand, within RALBFv3_RS17955 at 534.478 kb on + strand, within RALBFv3_RS17955 at 534.478 kb on + strand, within RALBFv3_RS17955 at 534.479 kb on - strand, within RALBFv3_RS17955 at 534.479 kb on - strand, within RALBFv3_RS17955
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 532,485 + RALBFv3_RS17950 0.74 +0.4 532,486 - RALBFv3_RS17950 0.74 +0.2 532,486 - RALBFv3_RS17950 0.74 +0.8 532,486 - RALBFv3_RS17950 0.74 -0.2 532,486 - RALBFv3_RS17950 0.74 -0.3 532,486 - RALBFv3_RS17950 0.74 -0.6 532,505 - RALBFv3_RS17950 0.75 -2.0 532,570 + RALBFv3_RS17950 0.80 -0.2 532,570 + RALBFv3_RS17950 0.80 +0.1 532,571 - RALBFv3_RS17950 0.80 -0.1 532,571 - RALBFv3_RS17950 0.80 -0.1 532,634 + RALBFv3_RS17950 0.84 +1.8 532,658 + RALBFv3_RS17950 0.86 -0.3 532,675 - RALBFv3_RS17950 0.87 +1.1 532,675 - RALBFv3_RS17950 0.87 -0.7 532,675 - RALBFv3_RS17950 0.87 -0.2 532,724 + -1.5 532,728 + +0.8 532,728 + -0.7 532,728 + +0.3 532,728 + +0.4 532,729 - -0.3 532,729 - -0.2 532,729 - +0.2 532,868 + -0.4 532,868 + -0.2 532,904 + -0.1 532,904 + +1.1 532,904 + +0.3 532,904 + +0.2 532,905 - -0.2 532,905 - -0.2 532,905 - -0.2 533,047 - +0.8 533,181 - +0.0 533,279 + -0.3 533,611 - RALBFv3_RS24305 0.87 -0.8 533,678 + -0.3 533,678 + -0.1 533,679 - +0.2 533,783 + -0.3 533,783 + -0.2 533,783 + -0.6 533,784 - -0.6 533,784 - +0.6 533,784 - -0.0 533,784 - +0.5 533,784 - +0.4 533,785 + +0.2 533,785 + -0.7 533,785 + -0.2 533,786 - -0.5 533,885 + -0.6 533,885 + -0.4 533,885 + -0.2 533,885 + -0.2 533,885 + -0.4 533,885 + -0.1 533,886 - +0.0 533,886 - -0.8 533,886 - +0.1 533,905 + -0.5 533,905 + +0.3 533,906 - -0.5 533,906 - +0.2 533,906 - -0.2 533,942 - +0.5 533,942 - +0.8 533,955 + -0.7 533,955 + +0.1 533,955 + +0.8 533,955 + +0.2 533,956 - -0.0 533,956 - +0.3 533,956 - -0.2 533,956 - -0.1 534,250 + +2.2 534,250 + -0.6 534,250 + +0.3 534,251 - -0.3 534,251 - +1.0 534,251 - -0.7 534,251 - -1.5 534,251 - -0.5 534,251 - +0.2 534,251 - +0.4 534,319 + RALBFv3_RS17955 0.16 +0.2 534,346 + RALBFv3_RS17955 0.20 +0.1 534,346 + RALBFv3_RS17955 0.20 +0.8 534,346 + RALBFv3_RS17955 0.20 -0.4 534,346 + RALBFv3_RS17955 0.20 -0.7 534,346 + RALBFv3_RS17955 0.20 -0.8 534,346 + RALBFv3_RS17955 0.20 +0.8 534,346 + RALBFv3_RS17955 0.20 -0.2 534,347 - RALBFv3_RS17955 0.20 +0.8 534,347 - RALBFv3_RS17955 0.20 -0.1 534,347 - RALBFv3_RS17955 0.20 +0.1 534,347 - RALBFv3_RS17955 0.20 -0.3 534,347 - RALBFv3_RS17955 0.20 +0.3 534,347 - RALBFv3_RS17955 0.20 +0.2 534,347 - RALBFv3_RS17955 0.20 +0.3 534,347 - RALBFv3_RS17955 0.20 +0.5 534,478 + RALBFv3_RS17955 0.36 -0.3 534,478 + RALBFv3_RS17955 0.36 +0.8 534,479 - RALBFv3_RS17955 0.36 -1.7 534,479 - RALBFv3_RS17955 0.36 -0.2
Or see this region's nucleotide sequence