Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS23640

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS02420 and RALBFv3_RS23640 are separated by 343 nucleotidesRALBFv3_RS23640 and RALBFv3_RS02425 are separated by 216 nucleotides RALBFv3_RS02420: RALBFv3_RS02420 - methyl-accepting chemotaxis protein, at 538,968 to 540,533 _RS02420 RALBFv3_RS23640: RALBFv3_RS23640 - hypothetical protein, at 540,877 to 541,104 _RS23640 RALBFv3_RS02425: RALBFv3_RS02425 - helix-turn-helix transcriptional regulator, at 541,321 to 542,079 _RS02425 Position (kb) 540 541 542Strain fitness (log2 ratio) -1 0 1at 539.885 kb on + strand, within RALBFv3_RS02420at 539.886 kb on - strand, within RALBFv3_RS02420at 540.149 kb on - strand, within RALBFv3_RS02420at 540.509 kb on + strandat 540.647 kb on + strandat 540.651 kb on + strandat 540.666 kb on - strandat 541.018 kb on - strand, within RALBFv3_RS23640at 541.266 kb on - strandat 541.322 kb on + strandat 541.324 kb on + strandat 541.683 kb on - strand, within RALBFv3_RS02425at 541.742 kb on + strand, within RALBFv3_RS02425at 541.743 kb on - strand, within RALBFv3_RS02425at 541.806 kb on - strand, within RALBFv3_RS02425at 541.886 kb on + strand, within RALBFv3_RS02425at 541.886 kb on + strand, within RALBFv3_RS02425

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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539,885 + RALBFv3_RS02420 0.59 -0.9
539,886 - RALBFv3_RS02420 0.59 +0.1
540,149 - RALBFv3_RS02420 0.75 +1.0
540,509 + +0.0
540,647 + +0.3
540,651 + -0.9
540,666 - -0.1
541,018 - RALBFv3_RS23640 0.62 -1.0
541,266 - -0.6
541,322 + -0.0
541,324 + -1.0
541,683 - RALBFv3_RS02425 0.48 -0.2
541,742 + RALBFv3_RS02425 0.55 +1.3
541,743 - RALBFv3_RS02425 0.56 -0.0
541,806 - RALBFv3_RS02425 0.64 -0.1
541,886 + RALBFv3_RS02425 0.74 -0.2
541,886 + RALBFv3_RS02425 0.74 +0.6

Or see this region's nucleotide sequence