Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS23240 and RALBFv3_RS23245 are separated by 247 nucleotides RALBFv3_RS23245 and RALBFv3_RS23250 are separated by 294 nucleotides
RALBFv3_RS23240: RALBFv3_RS23240 - UTP--glucose-1-phosphate uridylyltransferase, at 1,996,866 to 1,997,825
_RS23240
RALBFv3_RS23245: RALBFv3_RS23245 - DNA-binding protein, at 1,998,073 to 1,998,900
_RS23245
RALBFv3_RS23250: RALBFv3_RS23250 - chromosome partitioning protein ParB, at 1,999,195 to 2,000,199
_RS23250
Position (kb)
1998
1999 Strain fitness (log2 ratio)
-1
0
1 at 1997.083 kb on + strand, within RALBFv3_RS23240 at 1997.197 kb on + strand, within RALBFv3_RS23240 at 1997.199 kb on - strand, within RALBFv3_RS23240 at 1997.200 kb on + strand, within RALBFv3_RS23240 at 1997.259 kb on - strand, within RALBFv3_RS23240 at 1997.312 kb on - strand, within RALBFv3_RS23240 at 1997.374 kb on + strand, within RALBFv3_RS23240 at 1997.374 kb on + strand, within RALBFv3_RS23240 at 1997.374 kb on + strand, within RALBFv3_RS23240 at 1997.375 kb on - strand, within RALBFv3_RS23240 at 1997.518 kb on + strand, within RALBFv3_RS23240 at 1997.518 kb on + strand, within RALBFv3_RS23240 at 1997.518 kb on + strand, within RALBFv3_RS23240 at 1997.518 kb on + strand, within RALBFv3_RS23240 at 1997.518 kb on + strand, within RALBFv3_RS23240 at 1997.518 kb on + strand, within RALBFv3_RS23240 at 1997.519 kb on - strand, within RALBFv3_RS23240 at 1997.519 kb on - strand, within RALBFv3_RS23240 at 1997.609 kb on - strand, within RALBFv3_RS23240 at 1997.609 kb on - strand, within RALBFv3_RS23240 at 1997.704 kb on + strand, within RALBFv3_RS23240 at 1997.947 kb on + strand at 1998.135 kb on - strand at 1998.279 kb on + strand, within RALBFv3_RS23245 at 1998.279 kb on + strand, within RALBFv3_RS23245 at 1998.280 kb on - strand, within RALBFv3_RS23245 at 1998.280 kb on - strand, within RALBFv3_RS23245 at 1998.737 kb on + strand, within RALBFv3_RS23245 at 1998.911 kb on - strand at 1999.196 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 1,997,083 + RALBFv3_RS23240 0.23 +0.8 1,997,197 + RALBFv3_RS23240 0.34 -1.4 1,997,199 - RALBFv3_RS23240 0.35 -0.0 1,997,200 + RALBFv3_RS23240 0.35 +0.8 1,997,259 - RALBFv3_RS23240 0.41 -0.5 1,997,312 - RALBFv3_RS23240 0.46 +0.4 1,997,374 + RALBFv3_RS23240 0.53 -0.1 1,997,374 + RALBFv3_RS23240 0.53 +0.0 1,997,374 + RALBFv3_RS23240 0.53 -0.1 1,997,375 - RALBFv3_RS23240 0.53 -0.2 1,997,518 + RALBFv3_RS23240 0.68 +0.5 1,997,518 + RALBFv3_RS23240 0.68 -0.1 1,997,518 + RALBFv3_RS23240 0.68 -0.5 1,997,518 + RALBFv3_RS23240 0.68 -0.1 1,997,518 + RALBFv3_RS23240 0.68 +0.3 1,997,518 + RALBFv3_RS23240 0.68 -0.2 1,997,519 - RALBFv3_RS23240 0.68 -0.2 1,997,519 - RALBFv3_RS23240 0.68 -0.2 1,997,609 - RALBFv3_RS23240 0.77 -0.0 1,997,609 - RALBFv3_RS23240 0.77 -0.3 1,997,704 + RALBFv3_RS23240 0.87 -0.3 1,997,947 + -0.4 1,998,135 - -1.0 1,998,279 + RALBFv3_RS23245 0.25 -0.2 1,998,279 + RALBFv3_RS23245 0.25 -0.4 1,998,280 - RALBFv3_RS23245 0.25 -0.8 1,998,280 - RALBFv3_RS23245 0.25 +0.8 1,998,737 + RALBFv3_RS23245 0.80 -1.2 1,998,911 - -1.1 1,999,196 + +0.8
Or see this region's nucleotide sequence