Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS23105

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS23095 and RALBFv3_RS23100 are separated by 132 nucleotidesRALBFv3_RS23100 and RALBFv3_RS23105 are separated by 507 nucleotidesRALBFv3_RS23105 and RALBFv3_RS23110 overlap by 4 nucleotidesRALBFv3_RS23110 and RALBFv3_RS23115 are separated by 96 nucleotides RALBFv3_RS23095: RALBFv3_RS23095 - N-acetyltransferase, at 1,959,475 to 1,959,735 _RS23095 RALBFv3_RS23100: RALBFv3_RS23100 - DNA-binding protein, at 1,959,868 to 1,960,155 _RS23100 RALBFv3_RS23105: RALBFv3_RS23105 - chemotaxis protein CheY, at 1,960,663 to 1,961,070 _RS23105 RALBFv3_RS23110: RALBFv3_RS23110 - protein phosphatase CheZ, at 1,961,067 to 1,961,729 _RS23110 RALBFv3_RS23115: RALBFv3_RS23115 - type III effector gala6 protein, at 1,961,826 to 1,963,538 _RS23115 Position (kb) 1960 1961 1962Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1959.731 kb on + strandat 1959.762 kb on + strandat 1959.764 kb on + strandat 1959.812 kb on + strandat 1959.813 kb on - strandat 1959.845 kb on + strandat 1959.877 kb on + strandat 1959.878 kb on - strandat 1960.064 kb on - strand, within RALBFv3_RS23100at 1960.380 kb on + strandat 1960.381 kb on - strandat 1960.381 kb on - strandat 1960.381 kb on - strandat 1960.381 kb on - strandat 1960.470 kb on + strandat 1960.471 kb on - strandat 1960.473 kb on - strandat 1960.510 kb on - strandat 1960.510 kb on - strandat 1960.522 kb on - strandat 1960.544 kb on - strandat 1960.573 kb on + strandat 1960.679 kb on - strandat 1960.679 kb on - strandat 1960.984 kb on + strand, within RALBFv3_RS23105at 1961.259 kb on + strand, within RALBFv3_RS23110at 1961.260 kb on - strand, within RALBFv3_RS23110at 1961.260 kb on - strand, within RALBFv3_RS23110at 1961.260 kb on - strand, within RALBFv3_RS23110at 1961.260 kb on - strand, within RALBFv3_RS23110at 1961.260 kb on - strand, within RALBFv3_RS23110at 1961.260 kb on - strand, within RALBFv3_RS23110at 1961.260 kb on - strand, within RALBFv3_RS23110

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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1,959,731 + +0.8
1,959,762 + +0.4
1,959,764 + +0.3
1,959,812 + -1.0
1,959,813 - +0.8
1,959,845 + -0.5
1,959,877 + -0.2
1,959,878 - -0.7
1,960,064 - RALBFv3_RS23100 0.68 -0.4
1,960,380 + +0.1
1,960,381 - +0.1
1,960,381 - +0.1
1,960,381 - +0.2
1,960,381 - -1.2
1,960,470 + -0.2
1,960,471 - -0.0
1,960,473 - -0.7
1,960,510 - +0.3
1,960,510 - -0.5
1,960,522 - -0.2
1,960,544 - +0.0
1,960,573 + +0.8
1,960,679 - -0.7
1,960,679 - -0.2
1,960,984 + RALBFv3_RS23105 0.79 -1.4
1,961,259 + RALBFv3_RS23110 0.29 +2.8
1,961,260 - RALBFv3_RS23110 0.29 -1.9
1,961,260 - RALBFv3_RS23110 0.29 +0.3
1,961,260 - RALBFv3_RS23110 0.29 -0.0
1,961,260 - RALBFv3_RS23110 0.29 -0.2
1,961,260 - RALBFv3_RS23110 0.29 +0.2
1,961,260 - RALBFv3_RS23110 0.29 +0.2
1,961,260 - RALBFv3_RS23110 0.29 +0.3

Or see this region's nucleotide sequence