Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS23095 and RALBFv3_RS23100 are separated by 132 nucleotides RALBFv3_RS23100 and RALBFv3_RS23105 are separated by 507 nucleotides RALBFv3_RS23105 and RALBFv3_RS23110 overlap by 4 nucleotides RALBFv3_RS23110 and RALBFv3_RS23115 are separated by 96 nucleotides
RALBFv3_RS23095: RALBFv3_RS23095 - N-acetyltransferase, at 1,959,475 to 1,959,735
_RS23095
RALBFv3_RS23100: RALBFv3_RS23100 - DNA-binding protein, at 1,959,868 to 1,960,155
_RS23100
RALBFv3_RS23105: RALBFv3_RS23105 - chemotaxis protein CheY, at 1,960,663 to 1,961,070
_RS23105
RALBFv3_RS23110: RALBFv3_RS23110 - protein phosphatase CheZ, at 1,961,067 to 1,961,729
_RS23110
RALBFv3_RS23115: RALBFv3_RS23115 - type III effector gala6 protein, at 1,961,826 to 1,963,538
_RS23115
Position (kb)
1960
1961
1962 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1959.731 kb on + strand at 1959.762 kb on + strand at 1959.764 kb on + strand at 1959.812 kb on + strand at 1959.813 kb on - strand at 1959.845 kb on + strand at 1959.877 kb on + strand at 1959.878 kb on - strand at 1960.064 kb on - strand, within RALBFv3_RS23100 at 1960.380 kb on + strand at 1960.381 kb on - strand at 1960.381 kb on - strand at 1960.381 kb on - strand at 1960.381 kb on - strand at 1960.470 kb on + strand at 1960.471 kb on - strand at 1960.473 kb on - strand at 1960.510 kb on - strand at 1960.510 kb on - strand at 1960.522 kb on - strand at 1960.544 kb on - strand at 1960.573 kb on + strand at 1960.679 kb on - strand at 1960.679 kb on - strand at 1960.984 kb on + strand, within RALBFv3_RS23105 at 1961.259 kb on + strand, within RALBFv3_RS23110 at 1961.260 kb on - strand, within RALBFv3_RS23110 at 1961.260 kb on - strand, within RALBFv3_RS23110 at 1961.260 kb on - strand, within RALBFv3_RS23110 at 1961.260 kb on - strand, within RALBFv3_RS23110 at 1961.260 kb on - strand, within RALBFv3_RS23110 at 1961.260 kb on - strand, within RALBFv3_RS23110 at 1961.260 kb on - strand, within RALBFv3_RS23110
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 1,959,731 + +0.8 1,959,762 + +0.4 1,959,764 + +0.3 1,959,812 + -1.0 1,959,813 - +0.8 1,959,845 + -0.5 1,959,877 + -0.2 1,959,878 - -0.7 1,960,064 - RALBFv3_RS23100 0.68 -0.4 1,960,380 + +0.1 1,960,381 - +0.1 1,960,381 - +0.1 1,960,381 - +0.2 1,960,381 - -1.2 1,960,470 + -0.2 1,960,471 - -0.0 1,960,473 - -0.7 1,960,510 - +0.3 1,960,510 - -0.5 1,960,522 - -0.2 1,960,544 - +0.0 1,960,573 + +0.8 1,960,679 - -0.7 1,960,679 - -0.2 1,960,984 + RALBFv3_RS23105 0.79 -1.4 1,961,259 + RALBFv3_RS23110 0.29 +2.8 1,961,260 - RALBFv3_RS23110 0.29 -1.9 1,961,260 - RALBFv3_RS23110 0.29 +0.3 1,961,260 - RALBFv3_RS23110 0.29 -0.0 1,961,260 - RALBFv3_RS23110 0.29 -0.2 1,961,260 - RALBFv3_RS23110 0.29 +0.2 1,961,260 - RALBFv3_RS23110 0.29 +0.2 1,961,260 - RALBFv3_RS23110 0.29 +0.3
Or see this region's nucleotide sequence