Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS22620

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS22615 and RALBFv3_RS22620 overlap by 104 nucleotidesRALBFv3_RS22620 and RALBFv3_RS22625 are separated by 50 nucleotidesRALBFv3_RS22625 and RALBFv3_RS22630 are separated by 30 nucleotides RALBFv3_RS22615: RALBFv3_RS22615 - non-ribosomal peptide synthetase, at 1,834,849 to 1,843,542 _RS22615 RALBFv3_RS22620: RALBFv3_RS22620 - hypothetical protein, at 1,843,439 to 1,843,822 _RS22620 RALBFv3_RS22625: RALBFv3_RS22625 - MbtH family protein, at 1,843,873 to 1,844,070 _RS22625 RALBFv3_RS22630: RALBFv3_RS22630 - MATE family efflux transporter, at 1,844,101 to 1,845,555 _RS22630 Position (kb) 1843 1844Strain fitness (log2 ratio) -1 0 1at 1842.442 kb on + strand, within RALBFv3_RS22615at 1842.691 kb on + strandat 1842.692 kb on - strandat 1842.692 kb on - strandat 1842.716 kb on - strandat 1842.716 kb on - strandat 1843.179 kb on - strandat 1843.250 kb on + strandat 1843.250 kb on + strandat 1843.250 kb on + strandat 1843.250 kb on + strandat 1843.251 kb on - strandat 1843.251 kb on - strandat 1843.251 kb on - strandat 1843.252 kb on + strandat 1843.563 kb on + strand, within RALBFv3_RS22620at 1844.021 kb on - strand, within RALBFv3_RS22625at 1844.021 kb on - strand, within RALBFv3_RS22625at 1844.207 kb on + strandat 1844.208 kb on - strandat 1844.416 kb on - strand, within RALBFv3_RS22630at 1844.528 kb on - strand, within RALBFv3_RS22630at 1844.736 kb on - strand, within RALBFv3_RS22630

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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1,842,442 + RALBFv3_RS22615 0.87 -0.3
1,842,691 + -0.2
1,842,692 - -0.0
1,842,692 - +0.6
1,842,716 - -0.0
1,842,716 - -0.2
1,843,179 - -0.4
1,843,250 + +0.8
1,843,250 + +0.8
1,843,250 + -0.5
1,843,250 + +0.3
1,843,251 - -0.1
1,843,251 - +0.2
1,843,251 - -0.2
1,843,252 + -0.7
1,843,563 + RALBFv3_RS22620 0.32 -1.0
1,844,021 - RALBFv3_RS22625 0.75 +0.8
1,844,021 - RALBFv3_RS22625 0.75 -0.2
1,844,207 + +0.0
1,844,208 - -0.4
1,844,416 - RALBFv3_RS22630 0.22 -0.2
1,844,528 - RALBFv3_RS22630 0.29 -0.5
1,844,736 - RALBFv3_RS22630 0.44 -0.5

Or see this region's nucleotide sequence