Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS22185

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS22180 and RALBFv3_RS24710 are separated by 110 nucleotidesRALBFv3_RS24710 and RALBFv3_RS22185 overlap by 18 nucleotidesRALBFv3_RS22185 and RALBFv3_RS22190 are separated by 230 nucleotides RALBFv3_RS22180: RALBFv3_RS22180 - serine hydrolase, at 1,719,363 to 1,720,718 _RS22180 RALBFv3_RS24710: RALBFv3_RS24710 - hypothetical protein, at 1,720,829 to 1,721,083 _RS24710 RALBFv3_RS22185: RALBFv3_RS22185 - glucan biosynthesis protein D, at 1,721,066 to 1,722,679 _RS22185 RALBFv3_RS22190: RALBFv3_RS22190 - DUF3757 domain-containing protein, at 1,722,910 to 1,723,290 _RS22190 Position (kb) 1721 1722 1723Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 1720.072 kb on + strand, within RALBFv3_RS22180at 1720.120 kb on + strand, within RALBFv3_RS22180at 1720.120 kb on + strand, within RALBFv3_RS22180at 1720.121 kb on - strand, within RALBFv3_RS22180at 1720.377 kb on - strand, within RALBFv3_RS22180at 1720.377 kb on - strand, within RALBFv3_RS22180at 1720.748 kb on + strandat 1720.749 kb on - strandat 1720.749 kb on - strandat 1720.826 kb on + strandat 1720.827 kb on - strandat 1720.830 kb on + strandat 1720.831 kb on - strandat 1720.831 kb on - strandat 1720.831 kb on - strandat 1721.020 kb on + strand, within RALBFv3_RS24710at 1721.020 kb on + strand, within RALBFv3_RS24710at 1721.020 kb on + strand, within RALBFv3_RS24710at 1721.021 kb on - strand, within RALBFv3_RS24710at 1721.021 kb on - strand, within RALBFv3_RS24710at 1721.021 kb on - strand, within RALBFv3_RS24710at 1721.021 kb on - strand, within RALBFv3_RS24710at 1721.021 kb on - strand, within RALBFv3_RS24710at 1721.051 kb on - strand, within RALBFv3_RS24710at 1721.051 kb on - strand, within RALBFv3_RS24710at 1721.051 kb on - strand, within RALBFv3_RS24710at 1721.234 kb on + strand, within RALBFv3_RS22185at 1721.234 kb on + strand, within RALBFv3_RS22185at 1721.235 kb on - strand, within RALBFv3_RS22185at 1721.283 kb on - strand, within RALBFv3_RS22185at 1721.451 kb on - strand, within RALBFv3_RS22185at 1721.451 kb on - strand, within RALBFv3_RS22185at 1721.597 kb on + strand, within RALBFv3_RS22185at 1721.622 kb on - strand, within RALBFv3_RS22185at 1721.622 kb on - strand, within RALBFv3_RS22185at 1721.622 kb on - strand, within RALBFv3_RS22185at 1721.622 kb on - strand, within RALBFv3_RS22185at 1721.627 kb on + strand, within RALBFv3_RS22185at 1721.627 kb on + strand, within RALBFv3_RS22185at 1721.627 kb on + strand, within RALBFv3_RS22185at 1721.628 kb on - strand, within RALBFv3_RS22185at 1721.753 kb on + strand, within RALBFv3_RS22185at 1721.753 kb on + strand, within RALBFv3_RS22185at 1721.897 kb on + strand, within RALBFv3_RS22185at 1721.897 kb on + strand, within RALBFv3_RS22185at 1721.898 kb on - strand, within RALBFv3_RS22185at 1722.101 kb on + strand, within RALBFv3_RS22185at 1722.101 kb on + strand, within RALBFv3_RS22185at 1722.101 kb on + strand, within RALBFv3_RS22185at 1722.101 kb on + strand, within RALBFv3_RS22185at 1722.102 kb on - strand, within RALBFv3_RS22185at 1722.102 kb on - strand, within RALBFv3_RS22185at 1722.120 kb on - strand, within RALBFv3_RS22185at 1722.491 kb on + strand, within RALBFv3_RS22185at 1722.492 kb on - strand, within RALBFv3_RS22185at 1722.492 kb on - strand, within RALBFv3_RS22185at 1722.492 kb on - strand, within RALBFv3_RS22185at 1722.492 kb on - strand, within RALBFv3_RS22185at 1722.492 kb on - strand, within RALBFv3_RS22185at 1722.492 kb on - strand, within RALBFv3_RS22185at 1722.633 kb on - strandat 1722.639 kb on - strandat 1723.064 kb on + strand, within RALBFv3_RS22190at 1723.064 kb on + strand, within RALBFv3_RS22190at 1723.064 kb on + strand, within RALBFv3_RS22190at 1723.065 kb on - strand, within RALBFv3_RS22190at 1723.098 kb on + strand, within RALBFv3_RS22190at 1723.098 kb on + strand, within RALBFv3_RS22190at 1723.190 kb on + strand, within RALBFv3_RS22190at 1723.191 kb on + strand, within RALBFv3_RS22190at 1723.194 kb on - strand, within RALBFv3_RS22190at 1723.208 kb on + strand, within RALBFv3_RS22190at 1723.209 kb on - strand, within RALBFv3_RS22190at 1723.209 kb on - strand, within RALBFv3_RS22190at 1723.231 kb on - strand, within RALBFv3_RS22190at 1723.272 kb on + strandat 1723.272 kb on + strandat 1723.272 kb on + strandat 1723.272 kb on + strandat 1723.273 kb on - strandat 1723.273 kb on - strandat 1723.452 kb on + strandat 1723.551 kb on - strandat 1723.586 kb on + strandat 1723.586 kb on + strandat 1723.653 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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1,720,072 + RALBFv3_RS22180 0.52 -1.8
1,720,120 + RALBFv3_RS22180 0.56 +1.4
1,720,120 + RALBFv3_RS22180 0.56 -0.3
1,720,121 - RALBFv3_RS22180 0.56 +0.8
1,720,377 - RALBFv3_RS22180 0.75 -0.6
1,720,377 - RALBFv3_RS22180 0.75 +0.8
1,720,748 + -0.4
1,720,749 - -1.2
1,720,749 - +0.1
1,720,826 + -0.4
1,720,827 - -0.1
1,720,830 + +1.2
1,720,831 - -0.8
1,720,831 - -1.0
1,720,831 - +0.8
1,721,020 + RALBFv3_RS24710 0.75 -1.8
1,721,020 + RALBFv3_RS24710 0.75 -0.1
1,721,020 + RALBFv3_RS24710 0.75 -0.6
1,721,021 - RALBFv3_RS24710 0.75 +0.9
1,721,021 - RALBFv3_RS24710 0.75 +0.6
1,721,021 - RALBFv3_RS24710 0.75 +0.9
1,721,021 - RALBFv3_RS24710 0.75 -0.1
1,721,021 - RALBFv3_RS24710 0.75 -0.2
1,721,051 - RALBFv3_RS24710 0.87 -3.6
1,721,051 - RALBFv3_RS24710 0.87 -0.6
1,721,051 - RALBFv3_RS24710 0.87 -1.7
1,721,234 + RALBFv3_RS22185 0.10 -2.3
1,721,234 + RALBFv3_RS22185 0.10 +0.8
1,721,235 - RALBFv3_RS22185 0.10 -3.7
1,721,283 - RALBFv3_RS22185 0.13 +0.8
1,721,451 - RALBFv3_RS22185 0.24 -3.0
1,721,451 - RALBFv3_RS22185 0.24 +0.8
1,721,597 + RALBFv3_RS22185 0.33 -2.0
1,721,622 - RALBFv3_RS22185 0.34 -2.5
1,721,622 - RALBFv3_RS22185 0.34 -2.9
1,721,622 - RALBFv3_RS22185 0.34 -0.8
1,721,622 - RALBFv3_RS22185 0.34 -3.3
1,721,627 + RALBFv3_RS22185 0.35 -3.5
1,721,627 + RALBFv3_RS22185 0.35 -0.2
1,721,627 + RALBFv3_RS22185 0.35 -1.5
1,721,628 - RALBFv3_RS22185 0.35 -1.8
1,721,753 + RALBFv3_RS22185 0.43 -3.7
1,721,753 + RALBFv3_RS22185 0.43 -1.5
1,721,897 + RALBFv3_RS22185 0.51 -2.9
1,721,897 + RALBFv3_RS22185 0.51 -3.8
1,721,898 - RALBFv3_RS22185 0.52 -2.8
1,722,101 + RALBFv3_RS22185 0.64 -2.5
1,722,101 + RALBFv3_RS22185 0.64 -3.3
1,722,101 + RALBFv3_RS22185 0.64 -2.5
1,722,101 + RALBFv3_RS22185 0.64 -0.2
1,722,102 - RALBFv3_RS22185 0.64 -2.5
1,722,102 - RALBFv3_RS22185 0.64 -3.3
1,722,120 - RALBFv3_RS22185 0.65 -2.5
1,722,491 + RALBFv3_RS22185 0.88 -2.9
1,722,492 - RALBFv3_RS22185 0.88 -1.2
1,722,492 - RALBFv3_RS22185 0.88 -4.0
1,722,492 - RALBFv3_RS22185 0.88 -3.5
1,722,492 - RALBFv3_RS22185 0.88 -4.3
1,722,492 - RALBFv3_RS22185 0.88 -2.3
1,722,492 - RALBFv3_RS22185 0.88 -0.2
1,722,633 - -3.1
1,722,639 - +0.8
1,723,064 + RALBFv3_RS22190 0.40 -0.5
1,723,064 + RALBFv3_RS22190 0.40 -0.0
1,723,064 + RALBFv3_RS22190 0.40 +0.3
1,723,065 - RALBFv3_RS22190 0.41 +0.0
1,723,098 + RALBFv3_RS22190 0.49 -1.1
1,723,098 + RALBFv3_RS22190 0.49 -0.9
1,723,190 + RALBFv3_RS22190 0.73 -0.5
1,723,191 + RALBFv3_RS22190 0.74 +0.4
1,723,194 - RALBFv3_RS22190 0.75 -0.2
1,723,208 + RALBFv3_RS22190 0.78 +1.2
1,723,209 - RALBFv3_RS22190 0.78 +0.2
1,723,209 - RALBFv3_RS22190 0.78 +0.3
1,723,231 - RALBFv3_RS22190 0.84 -0.4
1,723,272 + +0.4
1,723,272 + -0.4
1,723,272 + +0.1
1,723,272 + -0.2
1,723,273 - -0.4
1,723,273 - -0.6
1,723,452 + -0.4
1,723,551 - -1.1
1,723,586 + +0.2
1,723,586 + +0.3
1,723,653 + +0.4

Or see this region's nucleotide sequence