Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS22130

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS22125 and RALBFv3_RS24700 overlap by 5 nucleotidesRALBFv3_RS24700 and RALBFv3_RS22130 are separated by 79 nucleotidesRALBFv3_RS22130 and RALBFv3_RS22135 are separated by 143 nucleotidesRALBFv3_RS22135 and RALBFv3_RS22140 are separated by 110 nucleotides RALBFv3_RS22125: RALBFv3_RS22125 - alcohol dehydrogenase, at 1,704,663 to 1,705,689 _RS22125 RALBFv3_RS24700: RALBFv3_RS24700 - hypothetical protein, at 1,705,685 to 1,705,867 _RS24700 RALBFv3_RS22130: RALBFv3_RS22130 - phosphatidylserine decarboxylase, at 1,705,947 to 1,706,786 _RS22130 RALBFv3_RS22135: RALBFv3_RS22135 - N-acetyltransferase, at 1,706,930 to 1,707,466 _RS22135 RALBFv3_RS22140: RALBFv3_RS22140 - TIGR02677 family protein, at 1,707,577 to 1,709,130 _RS22140 Position (kb) 1705 1706 1707Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1705.062 kb on - strand, within RALBFv3_RS22125at 1705.311 kb on + strand, within RALBFv3_RS22125at 1705.312 kb on - strand, within RALBFv3_RS22125at 1705.312 kb on - strand, within RALBFv3_RS22125at 1705.312 kb on - strand, within RALBFv3_RS22125at 1705.528 kb on - strand, within RALBFv3_RS22125at 1705.528 kb on - strand, within RALBFv3_RS22125at 1705.685 kb on + strandat 1705.685 kb on + strandat 1705.685 kb on + strandat 1705.685 kb on + strandat 1705.685 kb on + strandat 1705.686 kb on - strandat 1705.686 kb on - strandat 1705.686 kb on - strandat 1705.686 kb on - strandat 1705.686 kb on - strandat 1705.686 kb on - strandat 1705.686 kb on - strandat 1705.686 kb on - strandat 1705.698 kb on + strandat 1705.752 kb on - strand, within RALBFv3_RS24700at 1705.752 kb on - strand, within RALBFv3_RS24700at 1705.904 kb on + strandat 1705.911 kb on + strandat 1705.911 kb on + strandat 1705.911 kb on + strandat 1705.912 kb on - strandat 1705.912 kb on - strandat 1705.917 kb on - strandat 1705.917 kb on - strandat 1705.933 kb on + strandat 1705.933 kb on + strandat 1705.934 kb on - strandat 1706.059 kb on + strand, within RALBFv3_RS22130at 1706.080 kb on - strand, within RALBFv3_RS22130at 1706.080 kb on - strand, within RALBFv3_RS22130at 1706.125 kb on - strand, within RALBFv3_RS22130at 1706.125 kb on - strand, within RALBFv3_RS22130at 1706.349 kb on + strand, within RALBFv3_RS22130at 1706.350 kb on - strand, within RALBFv3_RS22130at 1706.350 kb on - strand, within RALBFv3_RS22130at 1706.350 kb on - strand, within RALBFv3_RS22130at 1706.415 kb on + strand, within RALBFv3_RS22130at 1706.415 kb on + strand, within RALBFv3_RS22130at 1706.415 kb on + strand, within RALBFv3_RS22130at 1706.416 kb on - strand, within RALBFv3_RS22130at 1706.416 kb on - strand, within RALBFv3_RS22130at 1706.416 kb on - strand, within RALBFv3_RS22130at 1706.441 kb on + strand, within RALBFv3_RS22130at 1706.642 kb on + strand, within RALBFv3_RS22130at 1706.642 kb on + strand, within RALBFv3_RS22130at 1706.642 kb on + strand, within RALBFv3_RS22130at 1706.642 kb on + strand, within RALBFv3_RS22130at 1706.643 kb on - strand, within RALBFv3_RS22130at 1706.643 kb on - strand, within RALBFv3_RS22130at 1706.643 kb on - strand, within RALBFv3_RS22130at 1706.643 kb on - strand, within RALBFv3_RS22130at 1706.643 kb on - strand, within RALBFv3_RS22130at 1706.814 kb on + strandat 1706.814 kb on + strandat 1706.815 kb on - strandat 1706.916 kb on + strandat 1706.916 kb on + strandat 1706.916 kb on + strandat 1706.917 kb on - strandat 1707.017 kb on + strand, within RALBFv3_RS22135at 1707.018 kb on - strand, within RALBFv3_RS22135at 1707.018 kb on - strand, within RALBFv3_RS22135at 1707.055 kb on + strand, within RALBFv3_RS22135at 1707.081 kb on + strand, within RALBFv3_RS22135at 1707.082 kb on - strand, within RALBFv3_RS22135at 1707.196 kb on + strand, within RALBFv3_RS22135at 1707.259 kb on + strand, within RALBFv3_RS22135at 1707.259 kb on + strand, within RALBFv3_RS22135at 1707.260 kb on - strand, within RALBFv3_RS22135at 1707.260 kb on - strand, within RALBFv3_RS22135at 1707.260 kb on - strand, within RALBFv3_RS22135at 1707.268 kb on - strand, within RALBFv3_RS22135at 1707.303 kb on - strand, within RALBFv3_RS22135at 1707.316 kb on + strand, within RALBFv3_RS22135at 1707.317 kb on - strand, within RALBFv3_RS22135at 1707.317 kb on - strand, within RALBFv3_RS22135at 1707.317 kb on - strand, within RALBFv3_RS22135at 1707.317 kb on - strand, within RALBFv3_RS22135at 1707.330 kb on + strand, within RALBFv3_RS22135at 1707.331 kb on - strand, within RALBFv3_RS22135at 1707.331 kb on - strand, within RALBFv3_RS22135at 1707.331 kb on - strand, within RALBFv3_RS22135at 1707.383 kb on + strand, within RALBFv3_RS22135at 1707.405 kb on - strand, within RALBFv3_RS22135at 1707.443 kb on + strandat 1707.444 kb on - strandat 1707.444 kb on - strandat 1707.509 kb on - strandat 1707.514 kb on + strandat 1707.514 kb on + strandat 1707.524 kb on - strandat 1707.535 kb on - strandat 1707.553 kb on - strandat 1707.645 kb on + strandat 1707.645 kb on + strandat 1707.652 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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1,705,062 - RALBFv3_RS22125 0.39 +0.2
1,705,311 + RALBFv3_RS22125 0.63 +0.1
1,705,312 - RALBFv3_RS22125 0.63 -0.2
1,705,312 - RALBFv3_RS22125 0.63 -0.2
1,705,312 - RALBFv3_RS22125 0.63 +0.4
1,705,528 - RALBFv3_RS22125 0.84 -0.1
1,705,528 - RALBFv3_RS22125 0.84 -1.2
1,705,685 + -0.3
1,705,685 + +0.8
1,705,685 + -0.4
1,705,685 + +0.8
1,705,685 + +0.8
1,705,686 - +0.2
1,705,686 - -0.2
1,705,686 - -0.5
1,705,686 - +0.6
1,705,686 - +0.2
1,705,686 - -0.1
1,705,686 - -0.2
1,705,686 - -0.1
1,705,698 + +0.1
1,705,752 - RALBFv3_RS24700 0.37 +0.8
1,705,752 - RALBFv3_RS24700 0.37 +0.2
1,705,904 + -0.3
1,705,911 + +0.2
1,705,911 + -0.3
1,705,911 + -0.4
1,705,912 - -0.7
1,705,912 - -1.0
1,705,917 - -1.1
1,705,917 - -1.3
1,705,933 + +0.6
1,705,933 + -0.2
1,705,934 - -0.5
1,706,059 + RALBFv3_RS22130 0.13 -0.8
1,706,080 - RALBFv3_RS22130 0.16 -0.1
1,706,080 - RALBFv3_RS22130 0.16 -1.2
1,706,125 - RALBFv3_RS22130 0.21 -2.1
1,706,125 - RALBFv3_RS22130 0.21 -1.2
1,706,349 + RALBFv3_RS22130 0.48 -0.8
1,706,350 - RALBFv3_RS22130 0.48 -1.0
1,706,350 - RALBFv3_RS22130 0.48 -1.0
1,706,350 - RALBFv3_RS22130 0.48 -1.2
1,706,415 + RALBFv3_RS22130 0.56 -1.0
1,706,415 + RALBFv3_RS22130 0.56 -1.0
1,706,415 + RALBFv3_RS22130 0.56 -0.5
1,706,416 - RALBFv3_RS22130 0.56 -0.5
1,706,416 - RALBFv3_RS22130 0.56 -1.2
1,706,416 - RALBFv3_RS22130 0.56 -1.4
1,706,441 + RALBFv3_RS22130 0.59 -0.8
1,706,642 + RALBFv3_RS22130 0.83 -0.9
1,706,642 + RALBFv3_RS22130 0.83 -1.1
1,706,642 + RALBFv3_RS22130 0.83 -1.3
1,706,642 + RALBFv3_RS22130 0.83 -1.7
1,706,643 - RALBFv3_RS22130 0.83 -0.7
1,706,643 - RALBFv3_RS22130 0.83 -1.3
1,706,643 - RALBFv3_RS22130 0.83 -1.0
1,706,643 - RALBFv3_RS22130 0.83 -0.4
1,706,643 - RALBFv3_RS22130 0.83 +0.8
1,706,814 + -0.2
1,706,814 + +0.8
1,706,815 - -0.2
1,706,916 + +0.3
1,706,916 + +3.1
1,706,916 + -0.8
1,706,917 - +0.3
1,707,017 + RALBFv3_RS22135 0.16 +0.1
1,707,018 - RALBFv3_RS22135 0.16 +0.3
1,707,018 - RALBFv3_RS22135 0.16 -0.4
1,707,055 + RALBFv3_RS22135 0.23 +0.1
1,707,081 + RALBFv3_RS22135 0.28 -0.0
1,707,082 - RALBFv3_RS22135 0.28 -1.4
1,707,196 + RALBFv3_RS22135 0.50 +0.1
1,707,259 + RALBFv3_RS22135 0.61 -0.4
1,707,259 + RALBFv3_RS22135 0.61 -0.4
1,707,260 - RALBFv3_RS22135 0.61 +0.5
1,707,260 - RALBFv3_RS22135 0.61 -0.2
1,707,260 - RALBFv3_RS22135 0.61 -0.4
1,707,268 - RALBFv3_RS22135 0.63 +1.0
1,707,303 - RALBFv3_RS22135 0.69 +0.5
1,707,316 + RALBFv3_RS22135 0.72 -0.1
1,707,317 - RALBFv3_RS22135 0.72 -0.1
1,707,317 - RALBFv3_RS22135 0.72 +0.2
1,707,317 - RALBFv3_RS22135 0.72 -0.4
1,707,317 - RALBFv3_RS22135 0.72 -0.2
1,707,330 + RALBFv3_RS22135 0.74 +0.1
1,707,331 - RALBFv3_RS22135 0.75 +0.0
1,707,331 - RALBFv3_RS22135 0.75 -0.2
1,707,331 - RALBFv3_RS22135 0.75 +0.3
1,707,383 + RALBFv3_RS22135 0.84 -0.1
1,707,405 - RALBFv3_RS22135 0.88 -0.8
1,707,443 + +0.3
1,707,444 - -0.3
1,707,444 - -0.4
1,707,509 - -0.1
1,707,514 + +0.1
1,707,514 + -0.1
1,707,524 - +0.4
1,707,535 - +0.2
1,707,553 - -0.4
1,707,645 + -0.6
1,707,645 + -0.9
1,707,652 + -0.3

Or see this region's nucleotide sequence