Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS22125 and RALBFv3_RS24700 overlap by 5 nucleotides RALBFv3_RS24700 and RALBFv3_RS22130 are separated by 79 nucleotides RALBFv3_RS22130 and RALBFv3_RS22135 are separated by 143 nucleotides RALBFv3_RS22135 and RALBFv3_RS22140 are separated by 110 nucleotides
RALBFv3_RS22125: RALBFv3_RS22125 - alcohol dehydrogenase, at 1,704,663 to 1,705,689
_RS22125
RALBFv3_RS24700: RALBFv3_RS24700 - hypothetical protein, at 1,705,685 to 1,705,867
_RS24700
RALBFv3_RS22130: RALBFv3_RS22130 - phosphatidylserine decarboxylase, at 1,705,947 to 1,706,786
_RS22130
RALBFv3_RS22135: RALBFv3_RS22135 - N-acetyltransferase, at 1,706,930 to 1,707,466
_RS22135
RALBFv3_RS22140: RALBFv3_RS22140 - TIGR02677 family protein, at 1,707,577 to 1,709,130
_RS22140
Position (kb)
1705
1706
1707 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1705.062 kb on - strand, within RALBFv3_RS22125 at 1705.311 kb on + strand, within RALBFv3_RS22125 at 1705.312 kb on - strand, within RALBFv3_RS22125 at 1705.312 kb on - strand, within RALBFv3_RS22125 at 1705.312 kb on - strand, within RALBFv3_RS22125 at 1705.528 kb on - strand, within RALBFv3_RS22125 at 1705.528 kb on - strand, within RALBFv3_RS22125 at 1705.685 kb on + strand at 1705.685 kb on + strand at 1705.685 kb on + strand at 1705.685 kb on + strand at 1705.685 kb on + strand at 1705.686 kb on - strand at 1705.686 kb on - strand at 1705.686 kb on - strand at 1705.686 kb on - strand at 1705.686 kb on - strand at 1705.686 kb on - strand at 1705.686 kb on - strand at 1705.686 kb on - strand at 1705.698 kb on + strand at 1705.752 kb on - strand, within RALBFv3_RS24700 at 1705.752 kb on - strand, within RALBFv3_RS24700 at 1705.904 kb on + strand at 1705.911 kb on + strand at 1705.911 kb on + strand at 1705.911 kb on + strand at 1705.912 kb on - strand at 1705.912 kb on - strand at 1705.917 kb on - strand at 1705.917 kb on - strand at 1705.933 kb on + strand at 1705.933 kb on + strand at 1705.934 kb on - strand at 1706.059 kb on + strand, within RALBFv3_RS22130 at 1706.080 kb on - strand, within RALBFv3_RS22130 at 1706.080 kb on - strand, within RALBFv3_RS22130 at 1706.125 kb on - strand, within RALBFv3_RS22130 at 1706.125 kb on - strand, within RALBFv3_RS22130 at 1706.349 kb on + strand, within RALBFv3_RS22130 at 1706.350 kb on - strand, within RALBFv3_RS22130 at 1706.350 kb on - strand, within RALBFv3_RS22130 at 1706.350 kb on - strand, within RALBFv3_RS22130 at 1706.415 kb on + strand, within RALBFv3_RS22130 at 1706.415 kb on + strand, within RALBFv3_RS22130 at 1706.415 kb on + strand, within RALBFv3_RS22130 at 1706.416 kb on - strand, within RALBFv3_RS22130 at 1706.416 kb on - strand, within RALBFv3_RS22130 at 1706.416 kb on - strand, within RALBFv3_RS22130 at 1706.441 kb on + strand, within RALBFv3_RS22130 at 1706.642 kb on + strand, within RALBFv3_RS22130 at 1706.642 kb on + strand, within RALBFv3_RS22130 at 1706.642 kb on + strand, within RALBFv3_RS22130 at 1706.642 kb on + strand, within RALBFv3_RS22130 at 1706.643 kb on - strand, within RALBFv3_RS22130 at 1706.643 kb on - strand, within RALBFv3_RS22130 at 1706.643 kb on - strand, within RALBFv3_RS22130 at 1706.643 kb on - strand, within RALBFv3_RS22130 at 1706.643 kb on - strand, within RALBFv3_RS22130 at 1706.814 kb on + strand at 1706.814 kb on + strand at 1706.815 kb on - strand at 1706.916 kb on + strand at 1706.916 kb on + strand at 1706.916 kb on + strand at 1706.917 kb on - strand at 1707.017 kb on + strand, within RALBFv3_RS22135 at 1707.018 kb on - strand, within RALBFv3_RS22135 at 1707.018 kb on - strand, within RALBFv3_RS22135 at 1707.055 kb on + strand, within RALBFv3_RS22135 at 1707.081 kb on + strand, within RALBFv3_RS22135 at 1707.082 kb on - strand, within RALBFv3_RS22135 at 1707.196 kb on + strand, within RALBFv3_RS22135 at 1707.259 kb on + strand, within RALBFv3_RS22135 at 1707.259 kb on + strand, within RALBFv3_RS22135 at 1707.260 kb on - strand, within RALBFv3_RS22135 at 1707.260 kb on - strand, within RALBFv3_RS22135 at 1707.260 kb on - strand, within RALBFv3_RS22135 at 1707.268 kb on - strand, within RALBFv3_RS22135 at 1707.303 kb on - strand, within RALBFv3_RS22135 at 1707.316 kb on + strand, within RALBFv3_RS22135 at 1707.317 kb on - strand, within RALBFv3_RS22135 at 1707.317 kb on - strand, within RALBFv3_RS22135 at 1707.317 kb on - strand, within RALBFv3_RS22135 at 1707.317 kb on - strand, within RALBFv3_RS22135 at 1707.330 kb on + strand, within RALBFv3_RS22135 at 1707.331 kb on - strand, within RALBFv3_RS22135 at 1707.331 kb on - strand, within RALBFv3_RS22135 at 1707.331 kb on - strand, within RALBFv3_RS22135 at 1707.383 kb on + strand, within RALBFv3_RS22135 at 1707.405 kb on - strand, within RALBFv3_RS22135 at 1707.443 kb on + strand at 1707.444 kb on - strand at 1707.444 kb on - strand at 1707.509 kb on - strand at 1707.514 kb on + strand at 1707.514 kb on + strand at 1707.524 kb on - strand at 1707.535 kb on - strand at 1707.553 kb on - strand at 1707.645 kb on + strand at 1707.645 kb on + strand at 1707.652 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 1,705,062 - RALBFv3_RS22125 0.39 +0.2 1,705,311 + RALBFv3_RS22125 0.63 +0.1 1,705,312 - RALBFv3_RS22125 0.63 -0.2 1,705,312 - RALBFv3_RS22125 0.63 -0.2 1,705,312 - RALBFv3_RS22125 0.63 +0.4 1,705,528 - RALBFv3_RS22125 0.84 -0.1 1,705,528 - RALBFv3_RS22125 0.84 -1.2 1,705,685 + -0.3 1,705,685 + +0.8 1,705,685 + -0.4 1,705,685 + +0.8 1,705,685 + +0.8 1,705,686 - +0.2 1,705,686 - -0.2 1,705,686 - -0.5 1,705,686 - +0.6 1,705,686 - +0.2 1,705,686 - -0.1 1,705,686 - -0.2 1,705,686 - -0.1 1,705,698 + +0.1 1,705,752 - RALBFv3_RS24700 0.37 +0.8 1,705,752 - RALBFv3_RS24700 0.37 +0.2 1,705,904 + -0.3 1,705,911 + +0.2 1,705,911 + -0.3 1,705,911 + -0.4 1,705,912 - -0.7 1,705,912 - -1.0 1,705,917 - -1.1 1,705,917 - -1.3 1,705,933 + +0.6 1,705,933 + -0.2 1,705,934 - -0.5 1,706,059 + RALBFv3_RS22130 0.13 -0.8 1,706,080 - RALBFv3_RS22130 0.16 -0.1 1,706,080 - RALBFv3_RS22130 0.16 -1.2 1,706,125 - RALBFv3_RS22130 0.21 -2.1 1,706,125 - RALBFv3_RS22130 0.21 -1.2 1,706,349 + RALBFv3_RS22130 0.48 -0.8 1,706,350 - RALBFv3_RS22130 0.48 -1.0 1,706,350 - RALBFv3_RS22130 0.48 -1.0 1,706,350 - RALBFv3_RS22130 0.48 -1.2 1,706,415 + RALBFv3_RS22130 0.56 -1.0 1,706,415 + RALBFv3_RS22130 0.56 -1.0 1,706,415 + RALBFv3_RS22130 0.56 -0.5 1,706,416 - RALBFv3_RS22130 0.56 -0.5 1,706,416 - RALBFv3_RS22130 0.56 -1.2 1,706,416 - RALBFv3_RS22130 0.56 -1.4 1,706,441 + RALBFv3_RS22130 0.59 -0.8 1,706,642 + RALBFv3_RS22130 0.83 -0.9 1,706,642 + RALBFv3_RS22130 0.83 -1.1 1,706,642 + RALBFv3_RS22130 0.83 -1.3 1,706,642 + RALBFv3_RS22130 0.83 -1.7 1,706,643 - RALBFv3_RS22130 0.83 -0.7 1,706,643 - RALBFv3_RS22130 0.83 -1.3 1,706,643 - RALBFv3_RS22130 0.83 -1.0 1,706,643 - RALBFv3_RS22130 0.83 -0.4 1,706,643 - RALBFv3_RS22130 0.83 +0.8 1,706,814 + -0.2 1,706,814 + +0.8 1,706,815 - -0.2 1,706,916 + +0.3 1,706,916 + +3.1 1,706,916 + -0.8 1,706,917 - +0.3 1,707,017 + RALBFv3_RS22135 0.16 +0.1 1,707,018 - RALBFv3_RS22135 0.16 +0.3 1,707,018 - RALBFv3_RS22135 0.16 -0.4 1,707,055 + RALBFv3_RS22135 0.23 +0.1 1,707,081 + RALBFv3_RS22135 0.28 -0.0 1,707,082 - RALBFv3_RS22135 0.28 -1.4 1,707,196 + RALBFv3_RS22135 0.50 +0.1 1,707,259 + RALBFv3_RS22135 0.61 -0.4 1,707,259 + RALBFv3_RS22135 0.61 -0.4 1,707,260 - RALBFv3_RS22135 0.61 +0.5 1,707,260 - RALBFv3_RS22135 0.61 -0.2 1,707,260 - RALBFv3_RS22135 0.61 -0.4 1,707,268 - RALBFv3_RS22135 0.63 +1.0 1,707,303 - RALBFv3_RS22135 0.69 +0.5 1,707,316 + RALBFv3_RS22135 0.72 -0.1 1,707,317 - RALBFv3_RS22135 0.72 -0.1 1,707,317 - RALBFv3_RS22135 0.72 +0.2 1,707,317 - RALBFv3_RS22135 0.72 -0.4 1,707,317 - RALBFv3_RS22135 0.72 -0.2 1,707,330 + RALBFv3_RS22135 0.74 +0.1 1,707,331 - RALBFv3_RS22135 0.75 +0.0 1,707,331 - RALBFv3_RS22135 0.75 -0.2 1,707,331 - RALBFv3_RS22135 0.75 +0.3 1,707,383 + RALBFv3_RS22135 0.84 -0.1 1,707,405 - RALBFv3_RS22135 0.88 -0.8 1,707,443 + +0.3 1,707,444 - -0.3 1,707,444 - -0.4 1,707,509 - -0.1 1,707,514 + +0.1 1,707,514 + -0.1 1,707,524 - +0.4 1,707,535 - +0.2 1,707,553 - -0.4 1,707,645 + -0.6 1,707,645 + -0.9 1,707,652 + -0.3
Or see this region's nucleotide sequence