Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS21475

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS21470 and RALBFv3_RS21475 are separated by 147 nucleotidesRALBFv3_RS21475 and RALBFv3_RS21480 are separated by 34 nucleotides RALBFv3_RS21470: RALBFv3_RS21470 - TonB-dependent siderophore receptor, at 1,541,172 to 1,543,409 _RS21470 RALBFv3_RS21475: RALBFv3_RS21475 - transcriptional regulator, at 1,543,557 to 1,543,997 _RS21475 RALBFv3_RS21480: RALBFv3_RS21480 - MFS transporter, at 1,544,032 to 1,545,267 _RS21480 Position (kb) 1543 1544Strain fitness (log2 ratio) -2 -1 0 1at 1542.684 kb on + strand, within RALBFv3_RS21470at 1542.685 kb on - strand, within RALBFv3_RS21470at 1542.751 kb on - strand, within RALBFv3_RS21470at 1542.759 kb on + strand, within RALBFv3_RS21470at 1542.760 kb on - strand, within RALBFv3_RS21470at 1542.831 kb on + strand, within RALBFv3_RS21470at 1542.831 kb on + strand, within RALBFv3_RS21470at 1542.846 kb on + strand, within RALBFv3_RS21470at 1543.179 kb on + strand, within RALBFv3_RS21470at 1543.180 kb on - strand, within RALBFv3_RS21470at 1543.194 kb on + strandat 1543.235 kb on + strandat 1543.235 kb on + strandat 1543.236 kb on - strandat 1543.327 kb on - strandat 1543.327 kb on - strandat 1543.576 kb on - strandat 1543.576 kb on - strandat 1543.576 kb on - strandat 1543.791 kb on + strand, within RALBFv3_RS21475at 1543.893 kb on + strand, within RALBFv3_RS21475at 1543.995 kb on + strandat 1544.118 kb on - strandat 1544.330 kb on + strand, within RALBFv3_RS21480at 1544.331 kb on - strand, within RALBFv3_RS21480at 1544.507 kb on + strand, within RALBFv3_RS21480at 1544.507 kb on + strand, within RALBFv3_RS21480at 1544.516 kb on + strand, within RALBFv3_RS21480at 1544.516 kb on + strand, within RALBFv3_RS21480at 1544.516 kb on + strand, within RALBFv3_RS21480at 1544.516 kb on + strand, within RALBFv3_RS21480at 1544.517 kb on - strand, within RALBFv3_RS21480at 1544.517 kb on - strand, within RALBFv3_RS21480at 1544.519 kb on + strand, within RALBFv3_RS21480at 1544.519 kb on + strand, within RALBFv3_RS21480at 1544.520 kb on - strand, within RALBFv3_RS21480at 1544.520 kb on - strand, within RALBFv3_RS21480at 1544.520 kb on - strand, within RALBFv3_RS21480at 1544.540 kb on + strand, within RALBFv3_RS21480at 1544.540 kb on + strand, within RALBFv3_RS21480at 1544.546 kb on + strand, within RALBFv3_RS21480at 1544.546 kb on + strand, within RALBFv3_RS21480at 1544.546 kb on + strand, within RALBFv3_RS21480at 1544.546 kb on + strand, within RALBFv3_RS21480at 1544.547 kb on - strand, within RALBFv3_RS21480at 1544.547 kb on - strand, within RALBFv3_RS21480at 1544.672 kb on + strand, within RALBFv3_RS21480at 1544.672 kb on + strand, within RALBFv3_RS21480at 1544.672 kb on + strand, within RALBFv3_RS21480at 1544.673 kb on - strand, within RALBFv3_RS21480

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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1,542,684 + RALBFv3_RS21470 0.68 -1.5
1,542,685 - RALBFv3_RS21470 0.68 -0.2
1,542,751 - RALBFv3_RS21470 0.71 +0.1
1,542,759 + RALBFv3_RS21470 0.71 +0.0
1,542,760 - RALBFv3_RS21470 0.71 -0.8
1,542,831 + RALBFv3_RS21470 0.74 -1.0
1,542,831 + RALBFv3_RS21470 0.74 +0.2
1,542,846 + RALBFv3_RS21470 0.75 +0.3
1,543,179 + RALBFv3_RS21470 0.90 -0.1
1,543,180 - RALBFv3_RS21470 0.90 -0.3
1,543,194 + +0.8
1,543,235 + -0.2
1,543,235 + +0.5
1,543,236 - -0.1
1,543,327 - -0.4
1,543,327 - -0.2
1,543,576 - -0.0
1,543,576 - -0.0
1,543,576 - +0.0
1,543,791 + RALBFv3_RS21475 0.53 -0.6
1,543,893 + RALBFv3_RS21475 0.76 -1.8
1,543,995 + -0.5
1,544,118 - +0.0
1,544,330 + RALBFv3_RS21480 0.24 +0.0
1,544,331 - RALBFv3_RS21480 0.24 -0.8
1,544,507 + RALBFv3_RS21480 0.38 -0.1
1,544,507 + RALBFv3_RS21480 0.38 -0.3
1,544,516 + RALBFv3_RS21480 0.39 -0.6
1,544,516 + RALBFv3_RS21480 0.39 +0.4
1,544,516 + RALBFv3_RS21480 0.39 -0.6
1,544,516 + RALBFv3_RS21480 0.39 -0.3
1,544,517 - RALBFv3_RS21480 0.39 +0.2
1,544,517 - RALBFv3_RS21480 0.39 -0.1
1,544,519 + RALBFv3_RS21480 0.39 -0.2
1,544,519 + RALBFv3_RS21480 0.39 -0.1
1,544,520 - RALBFv3_RS21480 0.39 -0.2
1,544,520 - RALBFv3_RS21480 0.39 -0.3
1,544,520 - RALBFv3_RS21480 0.39 +1.1
1,544,540 + RALBFv3_RS21480 0.41 +0.1
1,544,540 + RALBFv3_RS21480 0.41 -0.1
1,544,546 + RALBFv3_RS21480 0.42 +0.7
1,544,546 + RALBFv3_RS21480 0.42 +0.1
1,544,546 + RALBFv3_RS21480 0.42 -0.2
1,544,546 + RALBFv3_RS21480 0.42 +0.6
1,544,547 - RALBFv3_RS21480 0.42 -0.4
1,544,547 - RALBFv3_RS21480 0.42 -0.1
1,544,672 + RALBFv3_RS21480 0.52 +0.4
1,544,672 + RALBFv3_RS21480 0.52 +0.8
1,544,672 + RALBFv3_RS21480 0.52 +0.1
1,544,673 - RALBFv3_RS21480 0.52 +0.2

Or see this region's nucleotide sequence