Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS21470 and RALBFv3_RS21475 are separated by 147 nucleotides RALBFv3_RS21475 and RALBFv3_RS21480 are separated by 34 nucleotides
RALBFv3_RS21470: RALBFv3_RS21470 - TonB-dependent siderophore receptor, at 1,541,172 to 1,543,409
_RS21470
RALBFv3_RS21475: RALBFv3_RS21475 - transcriptional regulator, at 1,543,557 to 1,543,997
_RS21475
RALBFv3_RS21480: RALBFv3_RS21480 - MFS transporter, at 1,544,032 to 1,545,267
_RS21480
Position (kb)
1543
1544 Strain fitness (log2 ratio)
-2
-1
0
1 at 1542.684 kb on + strand, within RALBFv3_RS21470 at 1542.685 kb on - strand, within RALBFv3_RS21470 at 1542.751 kb on - strand, within RALBFv3_RS21470 at 1542.759 kb on + strand, within RALBFv3_RS21470 at 1542.760 kb on - strand, within RALBFv3_RS21470 at 1542.831 kb on + strand, within RALBFv3_RS21470 at 1542.831 kb on + strand, within RALBFv3_RS21470 at 1542.846 kb on + strand, within RALBFv3_RS21470 at 1543.179 kb on + strand, within RALBFv3_RS21470 at 1543.180 kb on - strand, within RALBFv3_RS21470 at 1543.194 kb on + strand at 1543.235 kb on + strand at 1543.235 kb on + strand at 1543.236 kb on - strand at 1543.327 kb on - strand at 1543.327 kb on - strand at 1543.576 kb on - strand at 1543.576 kb on - strand at 1543.576 kb on - strand at 1543.791 kb on + strand, within RALBFv3_RS21475 at 1543.893 kb on + strand, within RALBFv3_RS21475 at 1543.995 kb on + strand at 1544.118 kb on - strand at 1544.330 kb on + strand, within RALBFv3_RS21480 at 1544.331 kb on - strand, within RALBFv3_RS21480 at 1544.507 kb on + strand, within RALBFv3_RS21480 at 1544.507 kb on + strand, within RALBFv3_RS21480 at 1544.516 kb on + strand, within RALBFv3_RS21480 at 1544.516 kb on + strand, within RALBFv3_RS21480 at 1544.516 kb on + strand, within RALBFv3_RS21480 at 1544.516 kb on + strand, within RALBFv3_RS21480 at 1544.517 kb on - strand, within RALBFv3_RS21480 at 1544.517 kb on - strand, within RALBFv3_RS21480 at 1544.519 kb on + strand, within RALBFv3_RS21480 at 1544.519 kb on + strand, within RALBFv3_RS21480 at 1544.520 kb on - strand, within RALBFv3_RS21480 at 1544.520 kb on - strand, within RALBFv3_RS21480 at 1544.520 kb on - strand, within RALBFv3_RS21480 at 1544.540 kb on + strand, within RALBFv3_RS21480 at 1544.540 kb on + strand, within RALBFv3_RS21480 at 1544.546 kb on + strand, within RALBFv3_RS21480 at 1544.546 kb on + strand, within RALBFv3_RS21480 at 1544.546 kb on + strand, within RALBFv3_RS21480 at 1544.546 kb on + strand, within RALBFv3_RS21480 at 1544.547 kb on - strand, within RALBFv3_RS21480 at 1544.547 kb on - strand, within RALBFv3_RS21480 at 1544.672 kb on + strand, within RALBFv3_RS21480 at 1544.672 kb on + strand, within RALBFv3_RS21480 at 1544.672 kb on + strand, within RALBFv3_RS21480 at 1544.673 kb on - strand, within RALBFv3_RS21480
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 1,542,684 + RALBFv3_RS21470 0.68 -1.5 1,542,685 - RALBFv3_RS21470 0.68 -0.2 1,542,751 - RALBFv3_RS21470 0.71 +0.1 1,542,759 + RALBFv3_RS21470 0.71 +0.0 1,542,760 - RALBFv3_RS21470 0.71 -0.8 1,542,831 + RALBFv3_RS21470 0.74 -1.0 1,542,831 + RALBFv3_RS21470 0.74 +0.2 1,542,846 + RALBFv3_RS21470 0.75 +0.3 1,543,179 + RALBFv3_RS21470 0.90 -0.1 1,543,180 - RALBFv3_RS21470 0.90 -0.3 1,543,194 + +0.8 1,543,235 + -0.2 1,543,235 + +0.5 1,543,236 - -0.1 1,543,327 - -0.4 1,543,327 - -0.2 1,543,576 - -0.0 1,543,576 - -0.0 1,543,576 - +0.0 1,543,791 + RALBFv3_RS21475 0.53 -0.6 1,543,893 + RALBFv3_RS21475 0.76 -1.8 1,543,995 + -0.5 1,544,118 - +0.0 1,544,330 + RALBFv3_RS21480 0.24 +0.0 1,544,331 - RALBFv3_RS21480 0.24 -0.8 1,544,507 + RALBFv3_RS21480 0.38 -0.1 1,544,507 + RALBFv3_RS21480 0.38 -0.3 1,544,516 + RALBFv3_RS21480 0.39 -0.6 1,544,516 + RALBFv3_RS21480 0.39 +0.4 1,544,516 + RALBFv3_RS21480 0.39 -0.6 1,544,516 + RALBFv3_RS21480 0.39 -0.3 1,544,517 - RALBFv3_RS21480 0.39 +0.2 1,544,517 - RALBFv3_RS21480 0.39 -0.1 1,544,519 + RALBFv3_RS21480 0.39 -0.2 1,544,519 + RALBFv3_RS21480 0.39 -0.1 1,544,520 - RALBFv3_RS21480 0.39 -0.2 1,544,520 - RALBFv3_RS21480 0.39 -0.3 1,544,520 - RALBFv3_RS21480 0.39 +1.1 1,544,540 + RALBFv3_RS21480 0.41 +0.1 1,544,540 + RALBFv3_RS21480 0.41 -0.1 1,544,546 + RALBFv3_RS21480 0.42 +0.7 1,544,546 + RALBFv3_RS21480 0.42 +0.1 1,544,546 + RALBFv3_RS21480 0.42 -0.2 1,544,546 + RALBFv3_RS21480 0.42 +0.6 1,544,547 - RALBFv3_RS21480 0.42 -0.4 1,544,547 - RALBFv3_RS21480 0.42 -0.1 1,544,672 + RALBFv3_RS21480 0.52 +0.4 1,544,672 + RALBFv3_RS21480 0.52 +0.8 1,544,672 + RALBFv3_RS21480 0.52 +0.1 1,544,673 - RALBFv3_RS21480 0.52 +0.2
Or see this region's nucleotide sequence