Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS21125 and RALBFv3_RS21130 are separated by 117 nucleotides RALBFv3_RS21130 and RALBFv3_RS21135 are separated by 147 nucleotides RALBFv3_RS21135 and RALBFv3_RS21140 overlap by 4 nucleotides RALBFv3_RS21140 and RALBFv3_RS21145 are separated by 132 nucleotides
RALBFv3_RS21125: RALBFv3_RS21125 - Rrf2 family transcriptional regulator, at 1,453,531 to 1,453,962
_RS21125
RALBFv3_RS21130: RALBFv3_RS21130 - NAD(P)-dependent oxidoreductase, at 1,454,080 to 1,454,691
_RS21130
RALBFv3_RS21135: RALBFv3_RS21135 - two-component sensor histidine kinase, at 1,454,839 to 1,456,317
_RS21135
RALBFv3_RS21140: RALBFv3_RS21140 - DNA-binding response regulator, at 1,456,314 to 1,456,979
_RS21140
RALBFv3_RS21145: RALBFv3_RS21145 - molecular chaperone DnaJ, at 1,457,112 to 1,459,040
_RS21145
Position (kb)
1454
1455
1456
1457 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1453.961 kb on - strand at 1453.994 kb on + strand at 1454.036 kb on + strand at 1454.037 kb on - strand at 1454.056 kb on + strand at 1454.057 kb on - strand at 1454.434 kb on + strand, within RALBFv3_RS21130 at 1454.620 kb on + strand, within RALBFv3_RS21130 at 1454.621 kb on - strand, within RALBFv3_RS21130 at 1454.686 kb on + strand at 1454.686 kb on + strand at 1454.704 kb on + strand at 1454.853 kb on + strand at 1454.854 kb on - strand at 1454.854 kb on - strand at 1454.854 kb on - strand at 1454.854 kb on - strand at 1454.854 kb on - strand at 1455.515 kb on + strand, within RALBFv3_RS21135 at 1455.515 kb on + strand, within RALBFv3_RS21135 at 1455.516 kb on - strand, within RALBFv3_RS21135 at 1455.516 kb on - strand, within RALBFv3_RS21135 at 1455.516 kb on - strand, within RALBFv3_RS21135 at 1455.836 kb on + strand, within RALBFv3_RS21135 at 1455.894 kb on + strand, within RALBFv3_RS21135 at 1456.000 kb on - strand, within RALBFv3_RS21135 at 1456.140 kb on - strand, within RALBFv3_RS21135 at 1456.140 kb on - strand, within RALBFv3_RS21135 at 1456.262 kb on + strand at 1456.262 kb on + strand at 1456.262 kb on + strand at 1456.262 kb on + strand at 1456.263 kb on - strand at 1456.263 kb on - strand at 1456.263 kb on - strand at 1456.336 kb on + strand at 1456.903 kb on + strand, within RALBFv3_RS21140 at 1456.904 kb on - strand, within RALBFv3_RS21140 at 1456.904 kb on - strand, within RALBFv3_RS21140 at 1457.038 kb on + strand at 1457.099 kb on + strand at 1457.100 kb on - strand at 1457.101 kb on + strand at 1457.102 kb on - strand at 1457.166 kb on - strand at 1457.181 kb on + strand at 1457.272 kb on + strand at 1457.272 kb on + strand at 1457.308 kb on + strand, within RALBFv3_RS21145 at 1457.308 kb on + strand, within RALBFv3_RS21145 at 1457.308 kb on + strand, within RALBFv3_RS21145 at 1457.308 kb on + strand, within RALBFv3_RS21145 at 1457.309 kb on - strand, within RALBFv3_RS21145 at 1457.309 kb on - strand, within RALBFv3_RS21145 at 1457.309 kb on - strand, within RALBFv3_RS21145
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 1,453,961 - -1.0 1,453,994 + -0.2 1,454,036 + -0.1 1,454,037 - -0.4 1,454,056 + -0.0 1,454,057 - -0.2 1,454,434 + RALBFv3_RS21130 0.58 -0.3 1,454,620 + RALBFv3_RS21130 0.88 +1.0 1,454,621 - RALBFv3_RS21130 0.88 +0.4 1,454,686 + -0.5 1,454,686 + +0.2 1,454,704 + -0.3 1,454,853 + +0.0 1,454,854 - +0.4 1,454,854 - -0.5 1,454,854 - +0.3 1,454,854 - +0.7 1,454,854 - -0.3 1,455,515 + RALBFv3_RS21135 0.46 -0.9 1,455,515 + RALBFv3_RS21135 0.46 -2.0 1,455,516 - RALBFv3_RS21135 0.46 -0.9 1,455,516 - RALBFv3_RS21135 0.46 -0.5 1,455,516 - RALBFv3_RS21135 0.46 -2.3 1,455,836 + RALBFv3_RS21135 0.67 -0.7 1,455,894 + RALBFv3_RS21135 0.71 -0.1 1,456,000 - RALBFv3_RS21135 0.78 -1.7 1,456,140 - RALBFv3_RS21135 0.88 -1.0 1,456,140 - RALBFv3_RS21135 0.88 -1.3 1,456,262 + -2.9 1,456,262 + -1.8 1,456,262 + -2.0 1,456,262 + -1.1 1,456,263 - -0.8 1,456,263 - -0.2 1,456,263 - -2.5 1,456,336 + -2.0 1,456,903 + RALBFv3_RS21140 0.88 -1.0 1,456,904 - RALBFv3_RS21140 0.89 -1.5 1,456,904 - RALBFv3_RS21140 0.89 -0.2 1,457,038 + -1.2 1,457,099 + -2.2 1,457,100 - +0.6 1,457,101 + -1.1 1,457,102 - +0.5 1,457,166 - -0.3 1,457,181 + -0.2 1,457,272 + -0.2 1,457,272 + -0.2 1,457,308 + RALBFv3_RS21145 0.10 -0.2 1,457,308 + RALBFv3_RS21145 0.10 +0.5 1,457,308 + RALBFv3_RS21145 0.10 +0.6 1,457,308 + RALBFv3_RS21145 0.10 +0.3 1,457,309 - RALBFv3_RS21145 0.10 +0.8 1,457,309 - RALBFv3_RS21145 0.10 -0.4 1,457,309 - RALBFv3_RS21145 0.10 +0.8
Or see this region's nucleotide sequence