Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS21135

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS21125 and RALBFv3_RS21130 are separated by 117 nucleotidesRALBFv3_RS21130 and RALBFv3_RS21135 are separated by 147 nucleotidesRALBFv3_RS21135 and RALBFv3_RS21140 overlap by 4 nucleotidesRALBFv3_RS21140 and RALBFv3_RS21145 are separated by 132 nucleotides RALBFv3_RS21125: RALBFv3_RS21125 - Rrf2 family transcriptional regulator, at 1,453,531 to 1,453,962 _RS21125 RALBFv3_RS21130: RALBFv3_RS21130 - NAD(P)-dependent oxidoreductase, at 1,454,080 to 1,454,691 _RS21130 RALBFv3_RS21135: RALBFv3_RS21135 - two-component sensor histidine kinase, at 1,454,839 to 1,456,317 _RS21135 RALBFv3_RS21140: RALBFv3_RS21140 - DNA-binding response regulator, at 1,456,314 to 1,456,979 _RS21140 RALBFv3_RS21145: RALBFv3_RS21145 - molecular chaperone DnaJ, at 1,457,112 to 1,459,040 _RS21145 Position (kb) 1454 1455 1456 1457Strain fitness (log2 ratio) -3 -2 -1 0 1at 1453.961 kb on - strandat 1453.994 kb on + strandat 1454.036 kb on + strandat 1454.037 kb on - strandat 1454.056 kb on + strandat 1454.057 kb on - strandat 1454.434 kb on + strand, within RALBFv3_RS21130at 1454.620 kb on + strand, within RALBFv3_RS21130at 1454.621 kb on - strand, within RALBFv3_RS21130at 1454.686 kb on + strandat 1454.686 kb on + strandat 1454.704 kb on + strandat 1454.853 kb on + strandat 1454.854 kb on - strandat 1454.854 kb on - strandat 1454.854 kb on - strandat 1454.854 kb on - strandat 1454.854 kb on - strandat 1455.515 kb on + strand, within RALBFv3_RS21135at 1455.515 kb on + strand, within RALBFv3_RS21135at 1455.516 kb on - strand, within RALBFv3_RS21135at 1455.516 kb on - strand, within RALBFv3_RS21135at 1455.516 kb on - strand, within RALBFv3_RS21135at 1455.836 kb on + strand, within RALBFv3_RS21135at 1455.894 kb on + strand, within RALBFv3_RS21135at 1456.000 kb on - strand, within RALBFv3_RS21135at 1456.140 kb on - strand, within RALBFv3_RS21135at 1456.140 kb on - strand, within RALBFv3_RS21135at 1456.262 kb on + strandat 1456.262 kb on + strandat 1456.262 kb on + strandat 1456.262 kb on + strandat 1456.263 kb on - strandat 1456.263 kb on - strandat 1456.263 kb on - strandat 1456.336 kb on + strandat 1456.903 kb on + strand, within RALBFv3_RS21140at 1456.904 kb on - strand, within RALBFv3_RS21140at 1456.904 kb on - strand, within RALBFv3_RS21140at 1457.038 kb on + strandat 1457.099 kb on + strandat 1457.100 kb on - strandat 1457.101 kb on + strandat 1457.102 kb on - strandat 1457.166 kb on - strandat 1457.181 kb on + strandat 1457.272 kb on + strandat 1457.272 kb on + strandat 1457.308 kb on + strand, within RALBFv3_RS21145at 1457.308 kb on + strand, within RALBFv3_RS21145at 1457.308 kb on + strand, within RALBFv3_RS21145at 1457.308 kb on + strand, within RALBFv3_RS21145at 1457.309 kb on - strand, within RALBFv3_RS21145at 1457.309 kb on - strand, within RALBFv3_RS21145at 1457.309 kb on - strand, within RALBFv3_RS21145

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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1,453,961 - -1.0
1,453,994 + -0.2
1,454,036 + -0.1
1,454,037 - -0.4
1,454,056 + -0.0
1,454,057 - -0.2
1,454,434 + RALBFv3_RS21130 0.58 -0.3
1,454,620 + RALBFv3_RS21130 0.88 +1.0
1,454,621 - RALBFv3_RS21130 0.88 +0.4
1,454,686 + -0.5
1,454,686 + +0.2
1,454,704 + -0.3
1,454,853 + +0.0
1,454,854 - +0.4
1,454,854 - -0.5
1,454,854 - +0.3
1,454,854 - +0.7
1,454,854 - -0.3
1,455,515 + RALBFv3_RS21135 0.46 -0.9
1,455,515 + RALBFv3_RS21135 0.46 -2.0
1,455,516 - RALBFv3_RS21135 0.46 -0.9
1,455,516 - RALBFv3_RS21135 0.46 -0.5
1,455,516 - RALBFv3_RS21135 0.46 -2.3
1,455,836 + RALBFv3_RS21135 0.67 -0.7
1,455,894 + RALBFv3_RS21135 0.71 -0.1
1,456,000 - RALBFv3_RS21135 0.78 -1.7
1,456,140 - RALBFv3_RS21135 0.88 -1.0
1,456,140 - RALBFv3_RS21135 0.88 -1.3
1,456,262 + -2.9
1,456,262 + -1.8
1,456,262 + -2.0
1,456,262 + -1.1
1,456,263 - -0.8
1,456,263 - -0.2
1,456,263 - -2.5
1,456,336 + -2.0
1,456,903 + RALBFv3_RS21140 0.88 -1.0
1,456,904 - RALBFv3_RS21140 0.89 -1.5
1,456,904 - RALBFv3_RS21140 0.89 -0.2
1,457,038 + -1.2
1,457,099 + -2.2
1,457,100 - +0.6
1,457,101 + -1.1
1,457,102 - +0.5
1,457,166 - -0.3
1,457,181 + -0.2
1,457,272 + -0.2
1,457,272 + -0.2
1,457,308 + RALBFv3_RS21145 0.10 -0.2
1,457,308 + RALBFv3_RS21145 0.10 +0.5
1,457,308 + RALBFv3_RS21145 0.10 +0.6
1,457,308 + RALBFv3_RS21145 0.10 +0.3
1,457,309 - RALBFv3_RS21145 0.10 +0.8
1,457,309 - RALBFv3_RS21145 0.10 -0.4
1,457,309 - RALBFv3_RS21145 0.10 +0.8

Or see this region's nucleotide sequence