Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS20925

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS20915 and RALBFv3_RS20920 are separated by 5 nucleotidesRALBFv3_RS20920 and RALBFv3_RS20925 are separated by 333 nucleotidesRALBFv3_RS20925 and RALBFv3_RS20930 are separated by 101 nucleotides RALBFv3_RS20915: RALBFv3_RS20915 - IS3 family transposase, at 1,400,756 to 1,401,214 _RS20915 RALBFv3_RS20920: RALBFv3_RS20920 - hypothetical protein, at 1,401,220 to 1,401,588 _RS20920 RALBFv3_RS20925: RALBFv3_RS20925 - endoribonuclease L-PSP, at 1,401,922 to 1,402,299 _RS20925 RALBFv3_RS20930: RALBFv3_RS20930 - amino acid permease, at 1,402,401 to 1,403,825 _RS20930 Position (kb) 1401 1402 1403Strain fitness (log2 ratio) -2 -1 0 1 2at 1401.002 kb on - strand, within RALBFv3_RS20915at 1401.204 kb on + strandat 1401.204 kb on + strandat 1401.204 kb on + strandat 1401.204 kb on + strandat 1401.204 kb on + strandat 1401.205 kb on - strandat 1401.459 kb on + strand, within RALBFv3_RS20920at 1401.459 kb on + strand, within RALBFv3_RS20920at 1401.504 kb on + strand, within RALBFv3_RS20920at 1401.504 kb on + strand, within RALBFv3_RS20920at 1401.653 kb on + strandat 1401.653 kb on + strandat 1401.653 kb on + strandat 1401.654 kb on - strandat 1401.654 kb on - strandat 1401.654 kb on - strandat 1401.660 kb on + strandat 1401.660 kb on + strandat 1401.660 kb on + strandat 1401.660 kb on + strandat 1401.661 kb on - strandat 1401.671 kb on + strandat 1401.672 kb on - strandat 1401.681 kb on - strandat 1401.860 kb on + strandat 1401.860 kb on + strandat 1401.861 kb on - strandat 1401.999 kb on - strand, within RALBFv3_RS20925at 1402.378 kb on + strandat 1402.379 kb on - strandat 1402.379 kb on - strandat 1402.379 kb on - strandat 1402.570 kb on + strand, within RALBFv3_RS20930at 1402.570 kb on + strand, within RALBFv3_RS20930at 1402.571 kb on - strand, within RALBFv3_RS20930at 1402.574 kb on + strand, within RALBFv3_RS20930at 1402.574 kb on + strand, within RALBFv3_RS20930at 1402.574 kb on + strand, within RALBFv3_RS20930at 1402.574 kb on + strand, within RALBFv3_RS20930at 1402.574 kb on + strand, within RALBFv3_RS20930at 1402.575 kb on - strand, within RALBFv3_RS20930at 1402.575 kb on - strand, within RALBFv3_RS20930at 1402.575 kb on - strand, within RALBFv3_RS20930at 1402.575 kb on - strand, within RALBFv3_RS20930at 1402.575 kb on - strand, within RALBFv3_RS20930at 1402.575 kb on - strand, within RALBFv3_RS20930at 1402.575 kb on - strand, within RALBFv3_RS20930at 1402.575 kb on - strand, within RALBFv3_RS20930at 1402.575 kb on - strand, within RALBFv3_RS20930at 1402.575 kb on - strand, within RALBFv3_RS20930at 1402.578 kb on + strand, within RALBFv3_RS20930at 1402.578 kb on + strand, within RALBFv3_RS20930at 1402.579 kb on - strand, within RALBFv3_RS20930at 1402.579 kb on - strand, within RALBFv3_RS20930at 1402.579 kb on - strand, within RALBFv3_RS20930at 1402.579 kb on - strand, within RALBFv3_RS20930at 1402.579 kb on - strand, within RALBFv3_RS20930at 1402.579 kb on - strand, within RALBFv3_RS20930at 1402.579 kb on - strand, within RALBFv3_RS20930at 1402.638 kb on + strand, within RALBFv3_RS20930at 1402.668 kb on + strand, within RALBFv3_RS20930at 1402.668 kb on + strand, within RALBFv3_RS20930at 1402.668 kb on + strand, within RALBFv3_RS20930at 1402.668 kb on + strand, within RALBFv3_RS20930at 1402.668 kb on + strand, within RALBFv3_RS20930at 1402.668 kb on + strand, within RALBFv3_RS20930at 1402.669 kb on - strand, within RALBFv3_RS20930at 1402.669 kb on - strand, within RALBFv3_RS20930at 1402.669 kb on - strand, within RALBFv3_RS20930at 1402.669 kb on - strand, within RALBFv3_RS20930at 1402.669 kb on - strand, within RALBFv3_RS20930at 1402.670 kb on - strand, within RALBFv3_RS20930at 1402.758 kb on - strand, within RALBFv3_RS20930at 1402.773 kb on + strand, within RALBFv3_RS20930at 1402.773 kb on + strand, within RALBFv3_RS20930at 1402.773 kb on + strand, within RALBFv3_RS20930at 1402.773 kb on + strand, within RALBFv3_RS20930at 1402.774 kb on - strand, within RALBFv3_RS20930at 1402.774 kb on - strand, within RALBFv3_RS20930at 1402.782 kb on + strand, within RALBFv3_RS20930at 1402.783 kb on - strand, within RALBFv3_RS20930at 1402.783 kb on - strand, within RALBFv3_RS20930at 1402.783 kb on - strand, within RALBFv3_RS20930at 1402.996 kb on + strand, within RALBFv3_RS20930at 1402.996 kb on + strand, within RALBFv3_RS20930at 1402.996 kb on + strand, within RALBFv3_RS20930at 1402.996 kb on + strand, within RALBFv3_RS20930at 1402.996 kb on + strand, within RALBFv3_RS20930at 1402.996 kb on + strand, within RALBFv3_RS20930at 1402.997 kb on - strand, within RALBFv3_RS20930at 1402.997 kb on - strand, within RALBFv3_RS20930at 1402.997 kb on - strand, within RALBFv3_RS20930at 1402.997 kb on - strand, within RALBFv3_RS20930at 1402.997 kb on - strand, within RALBFv3_RS20930at 1402.997 kb on - strand, within RALBFv3_RS20930at 1402.998 kb on - strand, within RALBFv3_RS20930at 1403.175 kb on + strand, within RALBFv3_RS20930at 1403.175 kb on + strand, within RALBFv3_RS20930at 1403.175 kb on + strand, within RALBFv3_RS20930at 1403.176 kb on - strand, within RALBFv3_RS20930at 1403.176 kb on - strand, within RALBFv3_RS20930at 1403.176 kb on - strand, within RALBFv3_RS20930at 1403.224 kb on + strand, within RALBFv3_RS20930at 1403.224 kb on + strand, within RALBFv3_RS20930at 1403.225 kb on - strand, within RALBFv3_RS20930at 1403.225 kb on - strand, within RALBFv3_RS20930

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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1,401,002 - RALBFv3_RS20915 0.54 +0.3
1,401,204 + -0.2
1,401,204 + -0.1
1,401,204 + -0.9
1,401,204 + -0.8
1,401,204 + +0.1
1,401,205 - +0.1
1,401,459 + RALBFv3_RS20920 0.65 -0.4
1,401,459 + RALBFv3_RS20920 0.65 +0.8
1,401,504 + RALBFv3_RS20920 0.77 -0.3
1,401,504 + RALBFv3_RS20920 0.77 -0.5
1,401,653 + -0.8
1,401,653 + -0.7
1,401,653 + -0.4
1,401,654 - -0.3
1,401,654 - +0.2
1,401,654 - +0.1
1,401,660 + -0.7
1,401,660 + -0.1
1,401,660 + -0.2
1,401,660 + -0.6
1,401,661 - -0.2
1,401,671 + -0.2
1,401,672 - +0.8
1,401,681 - +0.0
1,401,860 + -1.8
1,401,860 + -0.2
1,401,861 - +0.8
1,401,999 - RALBFv3_RS20925 0.20 -1.1
1,402,378 + -0.2
1,402,379 - +0.2
1,402,379 - +2.4
1,402,379 - +0.0
1,402,570 + RALBFv3_RS20930 0.12 -0.6
1,402,570 + RALBFv3_RS20930 0.12 -1.2
1,402,571 - RALBFv3_RS20930 0.12 +0.5
1,402,574 + RALBFv3_RS20930 0.12 +0.4
1,402,574 + RALBFv3_RS20930 0.12 -1.3
1,402,574 + RALBFv3_RS20930 0.12 +0.2
1,402,574 + RALBFv3_RS20930 0.12 -0.2
1,402,574 + RALBFv3_RS20930 0.12 -0.3
1,402,575 - RALBFv3_RS20930 0.12 -1.0
1,402,575 - RALBFv3_RS20930 0.12 -0.3
1,402,575 - RALBFv3_RS20930 0.12 +0.1
1,402,575 - RALBFv3_RS20930 0.12 +0.8
1,402,575 - RALBFv3_RS20930 0.12 +0.4
1,402,575 - RALBFv3_RS20930 0.12 -0.9
1,402,575 - RALBFv3_RS20930 0.12 -0.6
1,402,575 - RALBFv3_RS20930 0.12 +0.5
1,402,575 - RALBFv3_RS20930 0.12 +0.6
1,402,575 - RALBFv3_RS20930 0.12 -0.3
1,402,578 + RALBFv3_RS20930 0.12 +0.1
1,402,578 + RALBFv3_RS20930 0.12 +0.2
1,402,579 - RALBFv3_RS20930 0.12 -0.2
1,402,579 - RALBFv3_RS20930 0.12 +0.2
1,402,579 - RALBFv3_RS20930 0.12 -0.7
1,402,579 - RALBFv3_RS20930 0.12 -0.3
1,402,579 - RALBFv3_RS20930 0.12 -0.8
1,402,579 - RALBFv3_RS20930 0.12 -0.8
1,402,579 - RALBFv3_RS20930 0.12 -0.5
1,402,638 + RALBFv3_RS20930 0.17 -0.5
1,402,668 + RALBFv3_RS20930 0.19 -0.5
1,402,668 + RALBFv3_RS20930 0.19 +1.0
1,402,668 + RALBFv3_RS20930 0.19 -0.6
1,402,668 + RALBFv3_RS20930 0.19 +0.0
1,402,668 + RALBFv3_RS20930 0.19 +0.8
1,402,668 + RALBFv3_RS20930 0.19 +0.4
1,402,669 - RALBFv3_RS20930 0.19 +0.1
1,402,669 - RALBFv3_RS20930 0.19 -0.2
1,402,669 - RALBFv3_RS20930 0.19 -0.2
1,402,669 - RALBFv3_RS20930 0.19 -0.1
1,402,669 - RALBFv3_RS20930 0.19 -0.4
1,402,670 - RALBFv3_RS20930 0.19 +0.2
1,402,758 - RALBFv3_RS20930 0.25 -0.3
1,402,773 + RALBFv3_RS20930 0.26 +0.8
1,402,773 + RALBFv3_RS20930 0.26 +0.0
1,402,773 + RALBFv3_RS20930 0.26 +1.2
1,402,773 + RALBFv3_RS20930 0.26 -0.1
1,402,774 - RALBFv3_RS20930 0.26 -0.1
1,402,774 - RALBFv3_RS20930 0.26 +0.1
1,402,782 + RALBFv3_RS20930 0.27 -0.1
1,402,783 - RALBFv3_RS20930 0.27 -0.7
1,402,783 - RALBFv3_RS20930 0.27 +1.8
1,402,783 - RALBFv3_RS20930 0.27 -0.1
1,402,996 + RALBFv3_RS20930 0.42 +0.6
1,402,996 + RALBFv3_RS20930 0.42 -0.6
1,402,996 + RALBFv3_RS20930 0.42 -0.4
1,402,996 + RALBFv3_RS20930 0.42 -0.3
1,402,996 + RALBFv3_RS20930 0.42 -0.0
1,402,996 + RALBFv3_RS20930 0.42 -0.5
1,402,997 - RALBFv3_RS20930 0.42 -0.6
1,402,997 - RALBFv3_RS20930 0.42 -0.3
1,402,997 - RALBFv3_RS20930 0.42 -0.5
1,402,997 - RALBFv3_RS20930 0.42 -1.3
1,402,997 - RALBFv3_RS20930 0.42 +0.0
1,402,997 - RALBFv3_RS20930 0.42 -0.8
1,402,998 - RALBFv3_RS20930 0.42 -0.2
1,403,175 + RALBFv3_RS20930 0.54 +0.6
1,403,175 + RALBFv3_RS20930 0.54 -0.1
1,403,175 + RALBFv3_RS20930 0.54 -0.3
1,403,176 - RALBFv3_RS20930 0.54 -0.1
1,403,176 - RALBFv3_RS20930 0.54 +0.3
1,403,176 - RALBFv3_RS20930 0.54 -0.0
1,403,224 + RALBFv3_RS20930 0.58 -0.2
1,403,224 + RALBFv3_RS20930 0.58 -0.4
1,403,225 - RALBFv3_RS20930 0.58 -0.4
1,403,225 - RALBFv3_RS20930 0.58 +0.1

Or see this region's nucleotide sequence