Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS19810

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS19800 and RALBFv3_RS19805 are separated by 24 nucleotidesRALBFv3_RS19805 and RALBFv3_RS19810 are separated by 136 nucleotidesRALBFv3_RS19810 and RALBFv3_RS24455 overlap by 47 nucleotidesRALBFv3_RS24455 and RALBFv3_RS19815 are separated by 84 nucleotides RALBFv3_RS19800: RALBFv3_RS19800 - adeC/adeK/oprM family multidrug efflux complex outer membrane factor, at 1,122,669 to 1,124,165 _RS19800 RALBFv3_RS19805: RALBFv3_RS19805 - TetR family transcriptional regulator, at 1,124,190 to 1,124,876 _RS19805 RALBFv3_RS19810: RALBFv3_RS19810 - TetR/AcrR family transcriptional regulator, at 1,125,013 to 1,125,636 _RS19810 RALBFv3_RS24455: RALBFv3_RS24455 - hypothetical protein, at 1,125,590 to 1,125,814 _RS24455 RALBFv3_RS19815: RALBFv3_RS19815 - malate dehydrogenase (quinone), at 1,125,899 to 1,127,572 _RS19815 Position (kb) 1125 1126Strain fitness (log2 ratio) -1 0 1 2 3at 1124.027 kb on + strandat 1124.088 kb on + strandat 1124.088 kb on + strandat 1124.089 kb on - strandat 1124.334 kb on + strand, within RALBFv3_RS19805at 1124.424 kb on + strand, within RALBFv3_RS19805at 1124.963 kb on + strandat 1124.963 kb on + strandat 1124.963 kb on + strandat 1124.963 kb on + strandat 1124.964 kb on - strandat 1124.964 kb on - strandat 1124.964 kb on - strandat 1124.964 kb on - strandat 1124.964 kb on - strandat 1124.964 kb on - strandat 1124.964 kb on - strandat 1124.964 kb on - strandat 1125.145 kb on + strand, within RALBFv3_RS19810at 1125.146 kb on - strand, within RALBFv3_RS19810at 1125.146 kb on - strand, within RALBFv3_RS19810at 1125.493 kb on + strand, within RALBFv3_RS19810at 1125.638 kb on - strand, within RALBFv3_RS24455at 1125.720 kb on + strand, within RALBFv3_RS24455at 1125.901 kb on - strandat 1126.428 kb on + strand, within RALBFv3_RS19815at 1126.512 kb on + strand, within RALBFv3_RS19815at 1126.513 kb on - strand, within RALBFv3_RS19815at 1126.513 kb on - strand, within RALBFv3_RS19815at 1126.513 kb on - strand, within RALBFv3_RS19815at 1126.513 kb on - strand, within RALBFv3_RS19815

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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1,124,027 + +0.1
1,124,088 + +1.8
1,124,088 + -1.2
1,124,089 - -0.6
1,124,334 + RALBFv3_RS19805 0.21 -1.2
1,124,424 + RALBFv3_RS19805 0.34 -0.1
1,124,963 + -0.5
1,124,963 + +0.7
1,124,963 + +0.0
1,124,963 + +0.3
1,124,964 - +0.8
1,124,964 - -0.4
1,124,964 - -0.7
1,124,964 - -0.2
1,124,964 - +0.2
1,124,964 - -0.0
1,124,964 - -0.2
1,124,964 - +0.2
1,125,145 + RALBFv3_RS19810 0.21 +0.0
1,125,146 - RALBFv3_RS19810 0.21 +3.2
1,125,146 - RALBFv3_RS19810 0.21 -1.5
1,125,493 + RALBFv3_RS19810 0.77 -1.4
1,125,638 - RALBFv3_RS24455 0.21 -0.6
1,125,720 + RALBFv3_RS24455 0.58 +0.3
1,125,901 - -0.4
1,126,428 + RALBFv3_RS19815 0.32 +0.6
1,126,512 + RALBFv3_RS19815 0.37 -0.1
1,126,513 - RALBFv3_RS19815 0.37 +0.0
1,126,513 - RALBFv3_RS19815 0.37 -0.0
1,126,513 - RALBFv3_RS19815 0.37 +0.3
1,126,513 - RALBFv3_RS19815 0.37 +0.8

Or see this region's nucleotide sequence