Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS19800 and RALBFv3_RS19805 are separated by 24 nucleotides RALBFv3_RS19805 and RALBFv3_RS19810 are separated by 136 nucleotides RALBFv3_RS19810 and RALBFv3_RS24455 overlap by 47 nucleotides RALBFv3_RS24455 and RALBFv3_RS19815 are separated by 84 nucleotides
RALBFv3_RS19800: RALBFv3_RS19800 - adeC/adeK/oprM family multidrug efflux complex outer membrane factor, at 1,122,669 to 1,124,165
_RS19800
RALBFv3_RS19805: RALBFv3_RS19805 - TetR family transcriptional regulator, at 1,124,190 to 1,124,876
_RS19805
RALBFv3_RS19810: RALBFv3_RS19810 - TetR/AcrR family transcriptional regulator, at 1,125,013 to 1,125,636
_RS19810
RALBFv3_RS24455: RALBFv3_RS24455 - hypothetical protein, at 1,125,590 to 1,125,814
_RS24455
RALBFv3_RS19815: RALBFv3_RS19815 - malate dehydrogenase (quinone), at 1,125,899 to 1,127,572
_RS19815
Position (kb)
1125
1126 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1124.027 kb on + strand at 1124.088 kb on + strand at 1124.088 kb on + strand at 1124.089 kb on - strand at 1124.334 kb on + strand, within RALBFv3_RS19805 at 1124.424 kb on + strand, within RALBFv3_RS19805 at 1124.963 kb on + strand at 1124.963 kb on + strand at 1124.963 kb on + strand at 1124.963 kb on + strand at 1124.964 kb on - strand at 1124.964 kb on - strand at 1124.964 kb on - strand at 1124.964 kb on - strand at 1124.964 kb on - strand at 1124.964 kb on - strand at 1124.964 kb on - strand at 1124.964 kb on - strand at 1125.145 kb on + strand, within RALBFv3_RS19810 at 1125.146 kb on - strand, within RALBFv3_RS19810 at 1125.146 kb on - strand, within RALBFv3_RS19810 at 1125.493 kb on + strand, within RALBFv3_RS19810 at 1125.638 kb on - strand, within RALBFv3_RS24455 at 1125.720 kb on + strand, within RALBFv3_RS24455 at 1125.901 kb on - strand at 1126.428 kb on + strand, within RALBFv3_RS19815 at 1126.512 kb on + strand, within RALBFv3_RS19815 at 1126.513 kb on - strand, within RALBFv3_RS19815 at 1126.513 kb on - strand, within RALBFv3_RS19815 at 1126.513 kb on - strand, within RALBFv3_RS19815 at 1126.513 kb on - strand, within RALBFv3_RS19815
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 1,124,027 + +0.1 1,124,088 + +1.8 1,124,088 + -1.2 1,124,089 - -0.6 1,124,334 + RALBFv3_RS19805 0.21 -1.2 1,124,424 + RALBFv3_RS19805 0.34 -0.1 1,124,963 + -0.5 1,124,963 + +0.7 1,124,963 + +0.0 1,124,963 + +0.3 1,124,964 - +0.8 1,124,964 - -0.4 1,124,964 - -0.7 1,124,964 - -0.2 1,124,964 - +0.2 1,124,964 - -0.0 1,124,964 - -0.2 1,124,964 - +0.2 1,125,145 + RALBFv3_RS19810 0.21 +0.0 1,125,146 - RALBFv3_RS19810 0.21 +3.2 1,125,146 - RALBFv3_RS19810 0.21 -1.5 1,125,493 + RALBFv3_RS19810 0.77 -1.4 1,125,638 - RALBFv3_RS24455 0.21 -0.6 1,125,720 + RALBFv3_RS24455 0.58 +0.3 1,125,901 - -0.4 1,126,428 + RALBFv3_RS19815 0.32 +0.6 1,126,512 + RALBFv3_RS19815 0.37 -0.1 1,126,513 - RALBFv3_RS19815 0.37 +0.0 1,126,513 - RALBFv3_RS19815 0.37 -0.0 1,126,513 - RALBFv3_RS19815 0.37 +0.3 1,126,513 - RALBFv3_RS19815 0.37 +0.8
Or see this region's nucleotide sequence