Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS24390 and RALBFv3_RS18635 overlap by 77 nucleotides RALBFv3_RS18635 and RALBFv3_RS18640 are separated by 88 nucleotides RALBFv3_RS18640 and RALBFv3_RS24395 are separated by 80 nucleotides RALBFv3_RS24395 and RALBFv3_RS18650 are separated by 213 nucleotides
RALBFv3_RS24390: RALBFv3_RS24390 - NAD(P)-dependent oxidoreductase, at 713,541 to 714,458
_RS24390
RALBFv3_RS18635: RALBFv3_RS18635 - AsnC family transcriptional regulator, at 714,382 to 714,690
_RS18635
RALBFv3_RS18640: RALBFv3_RS18640 - IS5/IS1182 family transposase, at 714,779 to 715,127
_RS18640
RALBFv3_RS24395: RALBFv3_RS24395 - hypothetical protein, at 715,208 to 715,468
_RS24395
RALBFv3_RS18650: RALBFv3_RS18650 - LacI family transcriptional regulator, at 715,682 to 716,704
_RS18650
Position (kb) 714
715
716 Strain fitness (log2 ratio) -1
0
1 at 713.810 kb on - strand, within RALBFv3_RS24390 at 714.003 kb on + strand, within RALBFv3_RS24390 at 714.003 kb on + strand, within RALBFv3_RS24390 at 714.003 kb on + strand, within RALBFv3_RS24390 at 714.003 kb on + strand, within RALBFv3_RS24390 at 714.004 kb on - strand, within RALBFv3_RS24390 at 714.004 kb on - strand, within RALBFv3_RS24390 at 714.004 kb on - strand, within RALBFv3_RS24390 at 714.004 kb on - strand, within RALBFv3_RS24390 at 714.004 kb on - strand, within RALBFv3_RS24390 at 714.004 kb on - strand, within RALBFv3_RS24390 at 714.004 kb on - strand, within RALBFv3_RS24390 at 714.004 kb on - strand, within RALBFv3_RS24390 at 714.005 kb on + strand, within RALBFv3_RS24390 at 714.016 kb on - strand, within RALBFv3_RS24390 at 714.016 kb on - strand, within RALBFv3_RS24390 at 714.016 kb on - strand, within RALBFv3_RS24390 at 714.100 kb on + strand, within RALBFv3_RS24390 at 714.101 kb on - strand, within RALBFv3_RS24390 at 714.188 kb on - strand, within RALBFv3_RS24390 at 714.188 kb on - strand, within RALBFv3_RS24390 at 714.229 kb on - strand, within RALBFv3_RS24390 at 714.356 kb on + strand, within RALBFv3_RS24390 at 714.356 kb on + strand, within RALBFv3_RS24390 at 714.357 kb on - strand, within RALBFv3_RS24390 at 714.371 kb on + strand at 714.380 kb on + strand at 714.391 kb on + strand at 714.392 kb on - strand at 714.431 kb on + strand at 714.432 kb on - strand at 714.676 kb on - strand at 714.732 kb on - strand at 714.807 kb on + strand at 714.808 kb on - strand at 714.916 kb on + strand, within RALBFv3_RS18640 at 715.048 kb on - strand, within RALBFv3_RS18640 at 715.119 kb on - strand at 715.130 kb on + strand at 715.130 kb on + strand at 715.130 kb on + strand at 715.130 kb on + strand at 715.130 kb on + strand at 715.130 kb on + strand at 715.131 kb on - strand at 715.131 kb on - strand at 715.131 kb on - strand at 715.836 kb on - strand, within RALBFv3_RS18650 at 715.836 kb on - strand, within RALBFv3_RS18650 at 715.970 kb on + strand, within RALBFv3_RS18650 at 715.991 kb on - strand, within RALBFv3_RS18650 at 715.991 kb on - strand, within RALBFv3_RS18650 at 716.025 kb on - strand, within RALBFv3_RS18650 at 716.025 kb on - strand, within RALBFv3_RS18650
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 713,810 - RALBFv3_RS24390 0.29 +0.1 714,003 + RALBFv3_RS24390 0.50 -0.3 714,003 + RALBFv3_RS24390 0.50 -0.1 714,003 + RALBFv3_RS24390 0.50 -0.1 714,003 + RALBFv3_RS24390 0.50 -1.1 714,004 - RALBFv3_RS24390 0.50 +0.0 714,004 - RALBFv3_RS24390 0.50 +0.6 714,004 - RALBFv3_RS24390 0.50 +1.0 714,004 - RALBFv3_RS24390 0.50 +0.0 714,004 - RALBFv3_RS24390 0.50 +0.0 714,004 - RALBFv3_RS24390 0.50 -0.1 714,004 - RALBFv3_RS24390 0.50 +0.0 714,004 - RALBFv3_RS24390 0.50 +0.7 714,005 + RALBFv3_RS24390 0.51 +0.4 714,016 - RALBFv3_RS24390 0.52 -1.6 714,016 - RALBFv3_RS24390 0.52 -0.1 714,016 - RALBFv3_RS24390 0.52 -0.5 714,100 + RALBFv3_RS24390 0.61 -0.2 714,101 - RALBFv3_RS24390 0.61 +0.0 714,188 - RALBFv3_RS24390 0.70 +0.8 714,188 - RALBFv3_RS24390 0.70 -0.2 714,229 - RALBFv3_RS24390 0.75 -0.0 714,356 + RALBFv3_RS24390 0.89 +0.5 714,356 + RALBFv3_RS24390 0.89 +0.3 714,357 - RALBFv3_RS24390 0.89 -0.1 714,371 + -0.1 714,380 + -0.3 714,391 + +0.3 714,392 - -0.2 714,431 + -0.4 714,432 - -0.6 714,676 - -0.5 714,732 - +0.8 714,807 + -0.9 714,808 - +0.1 714,916 + RALBFv3_RS18640 0.39 -0.9 715,048 - RALBFv3_RS18640 0.77 -1.0 715,119 - -0.7 715,130 + +0.3 715,130 + +0.3 715,130 + -0.4 715,130 + +0.1 715,130 + +0.5 715,130 + +1.0 715,131 - +0.2 715,131 - +0.7 715,131 - +0.1 715,836 - RALBFv3_RS18650 0.15 +0.6 715,836 - RALBFv3_RS18650 0.15 -1.6 715,970 + RALBFv3_RS18650 0.28 -0.6 715,991 - RALBFv3_RS18650 0.30 -1.5 715,991 - RALBFv3_RS18650 0.30 -0.2 716,025 - RALBFv3_RS18650 0.34 -0.0 716,025 - RALBFv3_RS18650 0.34 +0.5
Or see this region's nucleotide sequence