Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS18390

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS18385 and RALBFv3_RS18390 are separated by 281 nucleotidesRALBFv3_RS18390 and RALBFv3_RS18395 are separated by 49 nucleotides RALBFv3_RS18385: RALBFv3_RS18385 - alpha/beta hydrolase, at 650,411 to 651,493 _RS18385 RALBFv3_RS18390: RALBFv3_RS18390 - LysR family transcriptional regulator, at 651,775 to 652,719 _RS18390 RALBFv3_RS18395: RALBFv3_RS18395 - lytic murein transglycosylase, at 652,769 to 654,052 _RS18395 Position (kb) 651 652 653Strain fitness (log2 ratio) -2 -1 0 1 2at 651.166 kb on - strand, within RALBFv3_RS18385at 651.166 kb on - strand, within RALBFv3_RS18385at 651.166 kb on - strand, within RALBFv3_RS18385at 651.166 kb on - strand, within RALBFv3_RS18385at 651.253 kb on - strand, within RALBFv3_RS18385at 651.510 kb on + strandat 651.510 kb on + strandat 651.556 kb on + strandat 651.580 kb on + strandat 651.594 kb on + strandat 651.594 kb on + strandat 651.594 kb on + strandat 651.693 kb on + strandat 651.694 kb on - strandat 651.694 kb on - strandat 651.858 kb on - strandat 651.967 kb on + strand, within RALBFv3_RS18390at 651.970 kb on + strand, within RALBFv3_RS18390at 651.970 kb on + strand, within RALBFv3_RS18390at 651.970 kb on + strand, within RALBFv3_RS18390at 652.127 kb on - strand, within RALBFv3_RS18390at 652.127 kb on - strand, within RALBFv3_RS18390at 652.285 kb on + strand, within RALBFv3_RS18390at 652.348 kb on + strand, within RALBFv3_RS18390at 652.348 kb on + strand, within RALBFv3_RS18390at 652.348 kb on + strand, within RALBFv3_RS18390at 652.513 kb on + strand, within RALBFv3_RS18390at 652.513 kb on + strand, within RALBFv3_RS18390at 652.558 kb on + strand, within RALBFv3_RS18390at 652.592 kb on - strand, within RALBFv3_RS18390at 652.806 kb on + strandat 652.806 kb on + strandat 652.900 kb on - strand, within RALBFv3_RS18395at 652.900 kb on - strand, within RALBFv3_RS18395at 653.004 kb on + strand, within RALBFv3_RS18395at 653.037 kb on + strand, within RALBFv3_RS18395at 653.055 kb on + strand, within RALBFv3_RS18395at 653.056 kb on - strand, within RALBFv3_RS18395at 653.308 kb on - strand, within RALBFv3_RS18395at 653.308 kb on - strand, within RALBFv3_RS18395at 653.308 kb on - strand, within RALBFv3_RS18395at 653.385 kb on + strand, within RALBFv3_RS18395at 653.385 kb on + strand, within RALBFv3_RS18395at 653.386 kb on - strand, within RALBFv3_RS18395at 653.395 kb on - strand, within RALBFv3_RS18395at 653.565 kb on + strand, within RALBFv3_RS18395at 653.565 kb on + strand, within RALBFv3_RS18395at 653.566 kb on - strand, within RALBFv3_RS18395at 653.566 kb on - strand, within RALBFv3_RS18395at 653.621 kb on + strand, within RALBFv3_RS18395at 653.621 kb on + strand, within RALBFv3_RS18395at 653.622 kb on - strand, within RALBFv3_RS18395at 653.622 kb on - strand, within RALBFv3_RS18395at 653.641 kb on - strand, within RALBFv3_RS18395

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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651,166 - RALBFv3_RS18385 0.70 +1.4
651,166 - RALBFv3_RS18385 0.70 +0.8
651,166 - RALBFv3_RS18385 0.70 +0.8
651,166 - RALBFv3_RS18385 0.70 +0.8
651,253 - RALBFv3_RS18385 0.78 +1.8
651,510 + +0.8
651,510 + -0.2
651,556 + -0.7
651,580 + -0.4
651,594 + +0.8
651,594 + -1.0
651,594 + +0.4
651,693 + +0.8
651,694 - -0.4
651,694 - +0.1
651,858 - -0.5
651,967 + RALBFv3_RS18390 0.20 -0.8
651,970 + RALBFv3_RS18390 0.21 -1.8
651,970 + RALBFv3_RS18390 0.21 -0.9
651,970 + RALBFv3_RS18390 0.21 -1.0
652,127 - RALBFv3_RS18390 0.37 +0.8
652,127 - RALBFv3_RS18390 0.37 -1.2
652,285 + RALBFv3_RS18390 0.54 -0.9
652,348 + RALBFv3_RS18390 0.61 -0.6
652,348 + RALBFv3_RS18390 0.61 -0.6
652,348 + RALBFv3_RS18390 0.61 -0.7
652,513 + RALBFv3_RS18390 0.78 -0.6
652,513 + RALBFv3_RS18390 0.78 -0.9
652,558 + RALBFv3_RS18390 0.83 -0.3
652,592 - RALBFv3_RS18390 0.86 -0.3
652,806 + -0.0
652,806 + -2.2
652,900 - RALBFv3_RS18395 0.10 -0.1
652,900 - RALBFv3_RS18395 0.10 -1.0
653,004 + RALBFv3_RS18395 0.18 +0.1
653,037 + RALBFv3_RS18395 0.21 +0.3
653,055 + RALBFv3_RS18395 0.22 +0.3
653,056 - RALBFv3_RS18395 0.22 -0.2
653,308 - RALBFv3_RS18395 0.42 +0.2
653,308 - RALBFv3_RS18395 0.42 -0.8
653,308 - RALBFv3_RS18395 0.42 +0.3
653,385 + RALBFv3_RS18395 0.48 -0.2
653,385 + RALBFv3_RS18395 0.48 -0.2
653,386 - RALBFv3_RS18395 0.48 +1.3
653,395 - RALBFv3_RS18395 0.49 -0.2
653,565 + RALBFv3_RS18395 0.62 +0.5
653,565 + RALBFv3_RS18395 0.62 -0.5
653,566 - RALBFv3_RS18395 0.62 +0.5
653,566 - RALBFv3_RS18395 0.62 -0.2
653,621 + RALBFv3_RS18395 0.66 +0.0
653,621 + RALBFv3_RS18395 0.66 -0.4
653,622 - RALBFv3_RS18395 0.66 -0.1
653,622 - RALBFv3_RS18395 0.66 +0.2
653,641 - RALBFv3_RS18395 0.68 +0.0

Or see this region's nucleotide sequence