Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS18340 and RALBFv3_RS18345 are separated by 12 nucleotides RALBFv3_RS18345 and RALBFv3_RS18350 are separated by 10 nucleotides RALBFv3_RS18350 and RALBFv3_RS18355 are separated by 5 nucleotides RALBFv3_RS18355 and RALBFv3_RS18360 are separated by 13 nucleotides
RALBFv3_RS18340: RALBFv3_RS18340 - ArsR family transcriptional regulator, at 640,082 to 640,405
_RS18340
RALBFv3_RS18345: RALBFv3_RS18345 - glyoxalase/bleomycin resistance/dioxygenase family protein, at 640,418 to 640,891
_RS18345
RALBFv3_RS18350: RALBFv3_RS18350 - arsenate reductase ArsC, at 640,902 to 641,396
_RS18350
RALBFv3_RS18355: RALBFv3_RS18355 - aquaporin family protein, at 641,402 to 642,100
_RS18355
RALBFv3_RS18360: RALBFv3_RS18360 - transcriptional regulator ArgP, at 642,114 to 643,010
_RS18360
Position (kb) 640
641
642 Strain fitness (log2 ratio) -2
-1
0
1 at 640.298 kb on + strand, within RALBFv3_RS18340 at 640.299 kb on - strand, within RALBFv3_RS18340 at 640.435 kb on - strand at 640.475 kb on + strand, within RALBFv3_RS18345 at 640.475 kb on + strand, within RALBFv3_RS18345 at 640.476 kb on - strand, within RALBFv3_RS18345 at 640.520 kb on + strand, within RALBFv3_RS18345 at 640.520 kb on + strand, within RALBFv3_RS18345 at 640.663 kb on + strand, within RALBFv3_RS18345 at 640.700 kb on + strand, within RALBFv3_RS18345 at 640.934 kb on + strand at 640.935 kb on - strand at 641.010 kb on + strand, within RALBFv3_RS18350 at 641.010 kb on + strand, within RALBFv3_RS18350 at 641.011 kb on - strand, within RALBFv3_RS18350 at 641.558 kb on + strand, within RALBFv3_RS18355 at 641.558 kb on + strand, within RALBFv3_RS18355 at 641.559 kb on - strand, within RALBFv3_RS18355 at 641.559 kb on - strand, within RALBFv3_RS18355 at 641.559 kb on - strand, within RALBFv3_RS18355 at 641.559 kb on - strand, within RALBFv3_RS18355 at 641.864 kb on + strand, within RALBFv3_RS18355 at 641.864 kb on + strand, within RALBFv3_RS18355 at 641.865 kb on - strand, within RALBFv3_RS18355 at 641.866 kb on + strand, within RALBFv3_RS18355 at 641.880 kb on - strand, within RALBFv3_RS18355 at 641.944 kb on + strand, within RALBFv3_RS18355 at 641.944 kb on + strand, within RALBFv3_RS18355 at 641.945 kb on - strand, within RALBFv3_RS18355 at 642.071 kb on - strand at 642.098 kb on + strand at 642.098 kb on + strand at 642.098 kb on + strand at 642.099 kb on - strand at 642.196 kb on + strand at 642.213 kb on + strand, within RALBFv3_RS18360 at 642.214 kb on - strand, within RALBFv3_RS18360
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 640,298 + RALBFv3_RS18340 0.67 -1.6 640,299 - RALBFv3_RS18340 0.67 +0.1 640,435 - +0.9 640,475 + RALBFv3_RS18345 0.12 +0.1 640,475 + RALBFv3_RS18345 0.12 -0.2 640,476 - RALBFv3_RS18345 0.12 -0.3 640,520 + RALBFv3_RS18345 0.22 +0.7 640,520 + RALBFv3_RS18345 0.22 -0.2 640,663 + RALBFv3_RS18345 0.52 -0.4 640,700 + RALBFv3_RS18345 0.59 -0.2 640,934 + -0.3 640,935 - -0.0 641,010 + RALBFv3_RS18350 0.22 -0.5 641,010 + RALBFv3_RS18350 0.22 -1.2 641,011 - RALBFv3_RS18350 0.22 -0.0 641,558 + RALBFv3_RS18355 0.22 +1.1 641,558 + RALBFv3_RS18355 0.22 +0.1 641,559 - RALBFv3_RS18355 0.22 -0.2 641,559 - RALBFv3_RS18355 0.22 -1.3 641,559 - RALBFv3_RS18355 0.22 +0.4 641,559 - RALBFv3_RS18355 0.22 -0.2 641,864 + RALBFv3_RS18355 0.66 +0.2 641,864 + RALBFv3_RS18355 0.66 -0.2 641,865 - RALBFv3_RS18355 0.66 +1.2 641,866 + RALBFv3_RS18355 0.66 +0.5 641,880 - RALBFv3_RS18355 0.68 -0.4 641,944 + RALBFv3_RS18355 0.78 +0.8 641,944 + RALBFv3_RS18355 0.78 -0.2 641,945 - RALBFv3_RS18355 0.78 -0.2 642,071 - -0.4 642,098 + -0.7 642,098 + -2.5 642,098 + -0.1 642,099 - +0.4 642,196 + +0.4 642,213 + RALBFv3_RS18360 0.11 -0.6 642,214 - RALBFv3_RS18360 0.11 -0.4
Or see this region's nucleotide sequence