Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS18135 and RALBFv3_RS18140 are separated by 120 nucleotides RALBFv3_RS18140 and RALBFv3_RS18145 are separated by 54 nucleotides RALBFv3_RS18145 and RALBFv3_RS18150 are separated by 186 nucleotides
RALBFv3_RS18135: RALBFv3_RS18135 - two-component sensor histidine kinase, at 595,544 to 596,833
_RS18135
RALBFv3_RS18140: RALBFv3_RS18140 - DUF333 domain-containing protein, at 596,954 to 597,409
_RS18140
RALBFv3_RS18145: RALBFv3_RS18145 - phospholipid carrier-dependent glycosyltransferase, at 597,464 to 599,119
_RS18145
RALBFv3_RS18150: RALBFv3_RS18150 - DNA-binding response regulator, at 599,306 to 599,575
_RS18150
Position (kb) 597
598
599
600 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 596.851 kb on + strand at 596.857 kb on - strand at 596.863 kb on - strand at 597.400 kb on + strand at 597.400 kb on + strand at 597.400 kb on + strand at 597.401 kb on - strand at 597.401 kb on - strand at 597.696 kb on + strand, within RALBFv3_RS18145 at 597.696 kb on + strand, within RALBFv3_RS18145 at 597.696 kb on + strand, within RALBFv3_RS18145 at 597.697 kb on - strand, within RALBFv3_RS18145 at 597.699 kb on + strand, within RALBFv3_RS18145 at 597.699 kb on + strand, within RALBFv3_RS18145 at 597.699 kb on + strand, within RALBFv3_RS18145 at 597.700 kb on - strand, within RALBFv3_RS18145 at 597.700 kb on - strand, within RALBFv3_RS18145 at 597.732 kb on + strand, within RALBFv3_RS18145 at 598.150 kb on + strand, within RALBFv3_RS18145 at 598.152 kb on + strand, within RALBFv3_RS18145 at 598.152 kb on + strand, within RALBFv3_RS18145 at 598.153 kb on - strand, within RALBFv3_RS18145 at 598.344 kb on + strand, within RALBFv3_RS18145 at 598.344 kb on + strand, within RALBFv3_RS18145 at 598.345 kb on - strand, within RALBFv3_RS18145 at 598.345 kb on - strand, within RALBFv3_RS18145 at 598.542 kb on + strand, within RALBFv3_RS18145 at 598.542 kb on + strand, within RALBFv3_RS18145 at 598.753 kb on - strand, within RALBFv3_RS18145 at 598.809 kb on + strand, within RALBFv3_RS18145 at 598.951 kb on - strand, within RALBFv3_RS18145 at 599.197 kb on + strand at 599.206 kb on - strand at 599.307 kb on + strand at 599.308 kb on - strand at 599.308 kb on - strand at 599.343 kb on + strand, within RALBFv3_RS18150 at 599.343 kb on - strand, within RALBFv3_RS18150 at 599.344 kb on - strand, within RALBFv3_RS18150 at 599.344 kb on - strand, within RALBFv3_RS18150 at 599.591 kb on + strand at 599.592 kb on - strand at 599.592 kb on - strand at 599.592 kb on - strand at 599.635 kb on + strand at 599.635 kb on + strand at 599.635 kb on + strand at 599.649 kb on + strand at 599.649 kb on + strand at 599.650 kb on - strand at 599.657 kb on + strand at 599.918 kb on - strand at 599.953 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 596,851 + -0.1 596,857 - +0.5 596,863 - -0.7 597,400 + +0.8 597,400 + -0.0 597,400 + -0.3 597,401 - +0.0 597,401 - -0.0 597,696 + RALBFv3_RS18145 0.14 -4.2 597,696 + RALBFv3_RS18145 0.14 -4.0 597,696 + RALBFv3_RS18145 0.14 -3.7 597,697 - RALBFv3_RS18145 0.14 -4.9 597,699 + RALBFv3_RS18145 0.14 -3.8 597,699 + RALBFv3_RS18145 0.14 -3.7 597,699 + RALBFv3_RS18145 0.14 -1.7 597,700 - RALBFv3_RS18145 0.14 +0.8 597,700 - RALBFv3_RS18145 0.14 -3.8 597,732 + RALBFv3_RS18145 0.16 -3.2 598,150 + RALBFv3_RS18145 0.41 -3.2 598,152 + RALBFv3_RS18145 0.42 -3.4 598,152 + RALBFv3_RS18145 0.42 -2.7 598,153 - RALBFv3_RS18145 0.42 -4.2 598,344 + RALBFv3_RS18145 0.53 -5.0 598,344 + RALBFv3_RS18145 0.53 -4.7 598,345 - RALBFv3_RS18145 0.53 -3.6 598,345 - RALBFv3_RS18145 0.53 -3.3 598,542 + RALBFv3_RS18145 0.65 -1.2 598,542 + RALBFv3_RS18145 0.65 -4.8 598,753 - RALBFv3_RS18145 0.78 -3.6 598,809 + RALBFv3_RS18145 0.81 -4.2 598,951 - RALBFv3_RS18145 0.90 -6.2 599,197 + -4.1 599,206 - +0.1 599,307 + +0.5 599,308 - -0.2 599,308 - -0.0 599,343 + RALBFv3_RS18150 0.14 -0.0 599,343 - RALBFv3_RS18150 0.14 -0.2 599,344 - RALBFv3_RS18150 0.14 -0.6 599,344 - RALBFv3_RS18150 0.14 -0.3 599,591 + -0.0 599,592 - +0.3 599,592 - +0.2 599,592 - -0.8 599,635 + +0.0 599,635 + +0.8 599,635 + -0.4 599,649 + +0.1 599,649 + +0.5 599,650 - +0.7 599,657 + -1.5 599,918 - -0.5 599,953 - +0.2
Or see this region's nucleotide sequence