Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS17850 and RALBFv3_RS17855 overlap by 4 nucleotides RALBFv3_RS17855 and RALBFv3_RS17860 are separated by 83 nucleotides RALBFv3_RS17860 and RALBFv3_RS17865 are separated by 2 nucleotides
RALBFv3_RS17850: RALBFv3_RS17850 - hypothetical protein, at 507,207 to 509,630
_RS17850
RALBFv3_RS17855: RALBFv3_RS17855 - ornithine aminotransferase, at 509,627 to 512,167
_RS17855
RALBFv3_RS17860: RALBFv3_RS17860 - NAD(P)-dependent oxidoreductase, at 512,251 to 512,961
_RS17860
RALBFv3_RS17865: RALBFv3_RS17865 - DeoR/GlpR transcriptional regulator, at 512,964 to 513,728
_RS17865
Position (kb) 509
510
511
512
513 Strain fitness (log2 ratio) -1
0
1 at 508.656 kb on + strand, within RALBFv3_RS17850 at 508.691 kb on + strand, within RALBFv3_RS17850 at 508.864 kb on + strand, within RALBFv3_RS17850 at 509.017 kb on - strand, within RALBFv3_RS17850 at 509.076 kb on + strand, within RALBFv3_RS17850 at 509.077 kb on - strand, within RALBFv3_RS17850 at 509.089 kb on - strand, within RALBFv3_RS17850 at 509.089 kb on - strand, within RALBFv3_RS17850 at 509.089 kb on - strand, within RALBFv3_RS17850 at 509.160 kb on + strand, within RALBFv3_RS17850 at 509.202 kb on + strand, within RALBFv3_RS17850 at 509.202 kb on + strand, within RALBFv3_RS17850 at 509.202 kb on + strand, within RALBFv3_RS17850 at 509.202 kb on + strand, within RALBFv3_RS17850 at 509.203 kb on - strand, within RALBFv3_RS17850 at 509.203 kb on - strand, within RALBFv3_RS17850 at 509.203 kb on - strand, within RALBFv3_RS17850 at 509.343 kb on + strand, within RALBFv3_RS17850 at 509.380 kb on - strand, within RALBFv3_RS17850 at 509.394 kb on + strand at 509.561 kb on - strand at 509.561 kb on - strand at 509.563 kb on - strand at 509.574 kb on - strand at 509.855 kb on + strand at 509.856 kb on - strand at 509.856 kb on - strand at 510.066 kb on - strand, within RALBFv3_RS17855 at 510.066 kb on - strand, within RALBFv3_RS17855 at 510.302 kb on + strand, within RALBFv3_RS17855 at 510.303 kb on - strand, within RALBFv3_RS17855 at 510.320 kb on + strand, within RALBFv3_RS17855 at 510.321 kb on - strand, within RALBFv3_RS17855 at 510.321 kb on - strand, within RALBFv3_RS17855 at 510.830 kb on + strand, within RALBFv3_RS17855 at 510.830 kb on + strand, within RALBFv3_RS17855 at 510.830 kb on + strand, within RALBFv3_RS17855 at 510.831 kb on - strand, within RALBFv3_RS17855 at 510.878 kb on - strand, within RALBFv3_RS17855 at 510.878 kb on - strand, within RALBFv3_RS17855 at 510.884 kb on - strand, within RALBFv3_RS17855 at 510.983 kb on + strand, within RALBFv3_RS17855 at 510.983 kb on + strand, within RALBFv3_RS17855 at 510.983 kb on + strand, within RALBFv3_RS17855 at 510.983 kb on + strand, within RALBFv3_RS17855 at 510.984 kb on - strand, within RALBFv3_RS17855 at 510.998 kb on + strand, within RALBFv3_RS17855 at 511.017 kb on - strand, within RALBFv3_RS17855 at 511.175 kb on + strand, within RALBFv3_RS17855 at 511.175 kb on + strand, within RALBFv3_RS17855 at 511.176 kb on - strand, within RALBFv3_RS17855 at 511.176 kb on - strand, within RALBFv3_RS17855 at 511.190 kb on + strand, within RALBFv3_RS17855 at 511.191 kb on - strand, within RALBFv3_RS17855 at 511.427 kb on + strand, within RALBFv3_RS17855 at 511.776 kb on - strand, within RALBFv3_RS17855 at 511.907 kb on + strand, within RALBFv3_RS17855 at 512.165 kb on + strand at 512.165 kb on + strand at 512.165 kb on + strand at 512.253 kb on - strand at 513.166 kb on + strand, within RALBFv3_RS17865
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 508,656 + RALBFv3_RS17850 0.60 +0.4 508,691 + RALBFv3_RS17850 0.61 +0.3 508,864 + RALBFv3_RS17850 0.68 -1.6 509,017 - RALBFv3_RS17850 0.75 +0.4 509,076 + RALBFv3_RS17850 0.77 +0.2 509,077 - RALBFv3_RS17850 0.77 -0.0 509,089 - RALBFv3_RS17850 0.78 -1.0 509,089 - RALBFv3_RS17850 0.78 -0.5 509,089 - RALBFv3_RS17850 0.78 +0.9 509,160 + RALBFv3_RS17850 0.81 +0.1 509,202 + RALBFv3_RS17850 0.82 +0.9 509,202 + RALBFv3_RS17850 0.82 +0.2 509,202 + RALBFv3_RS17850 0.82 -0.6 509,202 + RALBFv3_RS17850 0.82 -0.2 509,203 - RALBFv3_RS17850 0.82 +1.3 509,203 - RALBFv3_RS17850 0.82 -0.2 509,203 - RALBFv3_RS17850 0.82 +0.5 509,343 + RALBFv3_RS17850 0.88 +0.2 509,380 - RALBFv3_RS17850 0.90 -0.1 509,394 + +0.6 509,561 - -1.1 509,561 - -1.2 509,563 - -0.6 509,574 - +0.1 509,855 + -0.7 509,856 - -0.9 509,856 - +1.3 510,066 - RALBFv3_RS17855 0.17 -0.6 510,066 - RALBFv3_RS17855 0.17 -0.1 510,302 + RALBFv3_RS17855 0.27 -0.1 510,303 - RALBFv3_RS17855 0.27 +0.3 510,320 + RALBFv3_RS17855 0.27 +0.3 510,321 - RALBFv3_RS17855 0.27 -0.1 510,321 - RALBFv3_RS17855 0.27 -0.3 510,830 + RALBFv3_RS17855 0.47 -0.4 510,830 + RALBFv3_RS17855 0.47 -0.2 510,830 + RALBFv3_RS17855 0.47 -0.1 510,831 - RALBFv3_RS17855 0.47 +0.3 510,878 - RALBFv3_RS17855 0.49 -0.1 510,878 - RALBFv3_RS17855 0.49 -1.3 510,884 - RALBFv3_RS17855 0.49 -0.7 510,983 + RALBFv3_RS17855 0.53 -0.6 510,983 + RALBFv3_RS17855 0.53 -0.0 510,983 + RALBFv3_RS17855 0.53 +0.9 510,983 + RALBFv3_RS17855 0.53 -0.1 510,984 - RALBFv3_RS17855 0.53 -0.7 510,998 + RALBFv3_RS17855 0.54 +0.7 511,017 - RALBFv3_RS17855 0.55 +0.6 511,175 + RALBFv3_RS17855 0.61 +0.1 511,175 + RALBFv3_RS17855 0.61 +0.3 511,176 - RALBFv3_RS17855 0.61 -1.0 511,176 - RALBFv3_RS17855 0.61 -0.2 511,190 + RALBFv3_RS17855 0.62 +0.2 511,191 - RALBFv3_RS17855 0.62 -0.3 511,427 + RALBFv3_RS17855 0.71 +0.5 511,776 - RALBFv3_RS17855 0.85 +0.9 511,907 + RALBFv3_RS17855 0.90 +0.1 512,165 + +0.5 512,165 + -0.6 512,165 + +0.6 512,253 - -0.2 513,166 + RALBFv3_RS17865 0.26 -0.6
Or see this region's nucleotide sequence