Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS17835 and RALBFv3_RS17840 overlap by 4 nucleotides RALBFv3_RS17840 and RALBFv3_RS17845 are separated by 23 nucleotides RALBFv3_RS17845 and RALBFv3_RS24295 are separated by 235 nucleotides
RALBFv3_RS17835: RALBFv3_RS17835 - cytochrome bd ubiquinol oxidase subunit II, at 502,993 to 504,024
_RS17835
RALBFv3_RS17840: RALBFv3_RS17840 - cytochrome ubiquinol oxidase subunit I, at 504,021 to 505,409
_RS17840
RALBFv3_RS17845: RALBFv3_RS17845 - GbsR/MarR family transcriptional regulator, at 505,433 to 505,993
_RS17845
RALBFv3_RS24295: RALBFv3_RS24295 - hypothetical protein, at 506,229 to 506,414
_RS24295
Position (kb) 504
505
506 Strain fitness (log2 ratio) -2
-1
0
1 at 503.036 kb on + strand at 503.036 kb on + strand at 503.037 kb on - strand at 503.037 kb on - strand at 503.040 kb on + strand at 503.042 kb on + strand at 503.042 kb on + strand at 503.043 kb on - strand at 503.043 kb on - strand at 503.043 kb on - strand at 503.051 kb on + strand at 503.051 kb on + strand at 503.051 kb on + strand at 503.052 kb on - strand at 503.052 kb on - strand at 503.052 kb on - strand at 503.171 kb on + strand, within RALBFv3_RS17835 at 503.172 kb on - strand, within RALBFv3_RS17835 at 503.517 kb on - strand, within RALBFv3_RS17835 at 503.573 kb on + strand, within RALBFv3_RS17835 at 503.591 kb on + strand, within RALBFv3_RS17835 at 503.592 kb on - strand, within RALBFv3_RS17835 at 503.642 kb on + strand, within RALBFv3_RS17835 at 503.642 kb on + strand, within RALBFv3_RS17835 at 503.939 kb on + strand at 503.940 kb on - strand at 504.136 kb on + strand at 504.154 kb on + strand at 504.155 kb on - strand at 504.155 kb on - strand at 504.176 kb on - strand, within RALBFv3_RS17840 at 504.187 kb on + strand, within RALBFv3_RS17840 at 504.188 kb on - strand, within RALBFv3_RS17840 at 504.188 kb on - strand, within RALBFv3_RS17840 at 504.296 kb on - strand, within RALBFv3_RS17840 at 504.296 kb on - strand, within RALBFv3_RS17840 at 504.296 kb on - strand, within RALBFv3_RS17840 at 504.532 kb on + strand, within RALBFv3_RS17840 at 504.533 kb on - strand, within RALBFv3_RS17840 at 504.854 kb on + strand, within RALBFv3_RS17840 at 504.854 kb on + strand, within RALBFv3_RS17840 at 504.854 kb on + strand, within RALBFv3_RS17840 at 504.854 kb on + strand, within RALBFv3_RS17840 at 504.855 kb on - strand, within RALBFv3_RS17840 at 504.855 kb on - strand, within RALBFv3_RS17840 at 504.855 kb on - strand, within RALBFv3_RS17840 at 504.855 kb on - strand, within RALBFv3_RS17840 at 504.855 kb on - strand, within RALBFv3_RS17840 at 504.855 kb on - strand, within RALBFv3_RS17840 at 504.869 kb on - strand, within RALBFv3_RS17840 at 505.115 kb on - strand, within RALBFv3_RS17840 at 505.156 kb on + strand, within RALBFv3_RS17840 at 505.417 kb on + strand at 505.418 kb on - strand at 505.435 kb on - strand at 505.435 kb on - strand at 505.587 kb on + strand, within RALBFv3_RS17845 at 505.587 kb on + strand, within RALBFv3_RS17845 at 505.587 kb on + strand, within RALBFv3_RS17845 at 505.587 kb on + strand, within RALBFv3_RS17845 at 505.587 kb on + strand, within RALBFv3_RS17845 at 505.587 kb on + strand, within RALBFv3_RS17845 at 505.587 kb on + strand, within RALBFv3_RS17845 at 505.587 kb on + strand, within RALBFv3_RS17845 at 505.749 kb on + strand, within RALBFv3_RS17845 at 505.750 kb on - strand, within RALBFv3_RS17845 at 505.750 kb on - strand, within RALBFv3_RS17845 at 505.890 kb on + strand, within RALBFv3_RS17845 at 505.891 kb on - strand, within RALBFv3_RS17845 at 505.891 kb on - strand, within RALBFv3_RS17845 at 505.902 kb on + strand, within RALBFv3_RS17845 at 505.902 kb on + strand, within RALBFv3_RS17845 at 505.903 kb on - strand, within RALBFv3_RS17845 at 506.166 kb on - strand at 506.166 kb on - strand at 506.166 kb on - strand at 506.166 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 503,036 + +0.5 503,036 + -0.4 503,037 - -1.3 503,037 - -0.8 503,040 + -1.1 503,042 + -0.1 503,042 + -2.5 503,043 - -1.3 503,043 - -0.8 503,043 - -1.6 503,051 + -1.3 503,051 + -2.6 503,051 + -0.9 503,052 - -1.5 503,052 - -0.8 503,052 - -0.8 503,171 + RALBFv3_RS17835 0.17 -0.1 503,172 - RALBFv3_RS17835 0.17 -1.4 503,517 - RALBFv3_RS17835 0.51 -0.1 503,573 + RALBFv3_RS17835 0.56 -0.5 503,591 + RALBFv3_RS17835 0.58 +0.9 503,592 - RALBFv3_RS17835 0.58 -1.0 503,642 + RALBFv3_RS17835 0.63 -0.1 503,642 + RALBFv3_RS17835 0.63 +0.9 503,939 + -1.0 503,940 - -1.2 504,136 + -1.3 504,154 + -1.3 504,155 - -1.1 504,155 - -1.4 504,176 - RALBFv3_RS17840 0.11 -0.7 504,187 + RALBFv3_RS17840 0.12 -1.1 504,188 - RALBFv3_RS17840 0.12 -0.9 504,188 - RALBFv3_RS17840 0.12 -0.6 504,296 - RALBFv3_RS17840 0.20 -1.2 504,296 - RALBFv3_RS17840 0.20 -0.7 504,296 - RALBFv3_RS17840 0.20 -0.7 504,532 + RALBFv3_RS17840 0.37 -2.1 504,533 - RALBFv3_RS17840 0.37 -0.5 504,854 + RALBFv3_RS17840 0.60 -1.5 504,854 + RALBFv3_RS17840 0.60 -0.6 504,854 + RALBFv3_RS17840 0.60 -1.5 504,854 + RALBFv3_RS17840 0.60 +0.9 504,855 - RALBFv3_RS17840 0.60 -1.1 504,855 - RALBFv3_RS17840 0.60 +0.9 504,855 - RALBFv3_RS17840 0.60 -1.3 504,855 - RALBFv3_RS17840 0.60 -0.1 504,855 - RALBFv3_RS17840 0.60 -1.1 504,855 - RALBFv3_RS17840 0.60 -1.7 504,869 - RALBFv3_RS17840 0.61 -1.0 505,115 - RALBFv3_RS17840 0.79 -2.1 505,156 + RALBFv3_RS17840 0.82 -0.7 505,417 + -0.9 505,418 - +0.4 505,435 - +0.3 505,435 - -0.1 505,587 + RALBFv3_RS17845 0.27 -1.0 505,587 + RALBFv3_RS17845 0.27 -1.4 505,587 + RALBFv3_RS17845 0.27 -0.6 505,587 + RALBFv3_RS17845 0.27 -1.6 505,587 + RALBFv3_RS17845 0.27 -0.2 505,587 + RALBFv3_RS17845 0.27 +0.7 505,587 + RALBFv3_RS17845 0.27 -0.7 505,587 + RALBFv3_RS17845 0.27 -2.3 505,749 + RALBFv3_RS17845 0.56 +0.9 505,750 - RALBFv3_RS17845 0.57 -0.6 505,750 - RALBFv3_RS17845 0.57 -0.2 505,890 + RALBFv3_RS17845 0.81 +0.9 505,891 - RALBFv3_RS17845 0.82 -0.5 505,891 - RALBFv3_RS17845 0.82 -0.1 505,902 + RALBFv3_RS17845 0.84 -2.3 505,902 + RALBFv3_RS17845 0.84 -0.6 505,903 - RALBFv3_RS17845 0.84 -0.7 506,166 - +0.4 506,166 - -0.3 506,166 - -0.4 506,166 - -0.1
Or see this region's nucleotide sequence