Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS17475 and RALBFv3_RS17480 are separated by 15 nucleotides RALBFv3_RS17480 and RALBFv3_RS17485 are separated by 9 nucleotides RALBFv3_RS17485 and RALBFv3_RS17490 are separated by 30 nucleotides
RALBFv3_RS17475: RALBFv3_RS17475 - flagellar biosynthesis protein FlhF, at 414,484 to 416,298
_RS17475
RALBFv3_RS17480: RALBFv3_RS17480 - flagellar synthesis regulator flen protein, at 416,314 to 417,081
_RS17480
RALBFv3_RS17485: RALBFv3_RS17485 - RNA polymerase sigma factor FliA, at 417,091 to 417,852
_RS17485
RALBFv3_RS17490: RALBFv3_RS17490 - MBL fold metallo-hydrolase, at 417,883 to 418,965
_RS17490
Position (kb) 416
417
418 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 415.525 kb on + strand, within RALBFv3_RS17475 at 415.525 kb on + strand, within RALBFv3_RS17475 at 415.525 kb on + strand, within RALBFv3_RS17475 at 415.525 kb on + strand, within RALBFv3_RS17475 at 415.526 kb on - strand, within RALBFv3_RS17475 at 415.526 kb on - strand, within RALBFv3_RS17475 at 415.526 kb on - strand, within RALBFv3_RS17475 at 415.765 kb on + strand, within RALBFv3_RS17475 at 415.766 kb on - strand at 415.766 kb on - strand, within RALBFv3_RS17475 at 415.767 kb on - strand, within RALBFv3_RS17475 at 415.767 kb on - strand, within RALBFv3_RS17475 at 416.111 kb on - strand, within RALBFv3_RS17475 at 416.119 kb on + strand at 416.119 kb on + strand at 416.650 kb on + strand, within RALBFv3_RS17480 at 416.786 kb on - strand, within RALBFv3_RS17480 at 416.786 kb on - strand, within RALBFv3_RS17480 at 416.786 kb on - strand, within RALBFv3_RS17480 at 416.816 kb on - strand, within RALBFv3_RS17480 at 416.816 kb on - strand, within RALBFv3_RS17480 at 416.816 kb on - strand, within RALBFv3_RS17480 at 416.816 kb on - strand, within RALBFv3_RS17480 at 416.816 kb on - strand, within RALBFv3_RS17480 at 417.076 kb on + strand at 417.124 kb on + strand at 417.124 kb on + strand at 417.124 kb on + strand at 417.124 kb on + strand at 417.124 kb on + strand at 417.124 kb on + strand at 417.125 kb on - strand at 417.125 kb on - strand at 417.125 kb on - strand at 417.125 kb on - strand at 417.125 kb on - strand at 417.125 kb on - strand at 417.125 kb on - strand at 417.125 kb on - strand at 417.126 kb on - strand at 417.170 kb on - strand, within RALBFv3_RS17485 at 417.170 kb on - strand, within RALBFv3_RS17485 at 417.319 kb on + strand, within RALBFv3_RS17485 at 417.319 kb on + strand, within RALBFv3_RS17485 at 417.544 kb on + strand, within RALBFv3_RS17485 at 417.545 kb on - strand, within RALBFv3_RS17485 at 417.547 kb on + strand, within RALBFv3_RS17485 at 417.547 kb on + strand, within RALBFv3_RS17485 at 417.548 kb on - strand, within RALBFv3_RS17485 at 417.710 kb on - strand, within RALBFv3_RS17485 at 417.713 kb on - strand, within RALBFv3_RS17485 at 417.713 kb on - strand, within RALBFv3_RS17485 at 417.885 kb on - strand at 417.885 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 415,525 + RALBFv3_RS17475 0.57 +0.8 415,525 + RALBFv3_RS17475 0.57 -0.2 415,525 + RALBFv3_RS17475 0.57 +0.1 415,525 + RALBFv3_RS17475 0.57 +0.1 415,526 - RALBFv3_RS17475 0.57 +0.4 415,526 - RALBFv3_RS17475 0.57 -1.2 415,526 - RALBFv3_RS17475 0.57 -0.8 415,765 + RALBFv3_RS17475 0.71 +0.7 415,766 - +0.4 415,766 - RALBFv3_RS17475 0.71 +0.3 415,767 - RALBFv3_RS17475 0.71 +1.0 415,767 - RALBFv3_RS17475 0.71 +0.6 416,111 - RALBFv3_RS17475 0.90 -0.3 416,119 + -0.6 416,119 + +1.2 416,650 + RALBFv3_RS17480 0.44 -1.5 416,786 - RALBFv3_RS17480 0.61 -1.5 416,786 - RALBFv3_RS17480 0.61 -1.4 416,786 - RALBFv3_RS17480 0.61 -0.6 416,816 - RALBFv3_RS17480 0.65 -3.4 416,816 - RALBFv3_RS17480 0.65 -1.2 416,816 - RALBFv3_RS17480 0.65 +0.8 416,816 - RALBFv3_RS17480 0.65 -1.2 416,816 - RALBFv3_RS17480 0.65 +0.8 417,076 + -0.5 417,124 + -0.3 417,124 + -0.2 417,124 + +0.4 417,124 + -0.2 417,124 + -0.7 417,124 + -0.9 417,125 - +0.0 417,125 - -0.4 417,125 - -0.9 417,125 - -0.4 417,125 - -0.3 417,125 - +0.1 417,125 - -0.4 417,125 - -0.7 417,126 - +0.0 417,170 - RALBFv3_RS17485 0.10 -0.8 417,170 - RALBFv3_RS17485 0.10 +0.8 417,319 + RALBFv3_RS17485 0.30 -0.4 417,319 + RALBFv3_RS17485 0.30 -1.4 417,544 + RALBFv3_RS17485 0.59 -0.4 417,545 - RALBFv3_RS17485 0.60 -0.1 417,547 + RALBFv3_RS17485 0.60 -0.3 417,547 + RALBFv3_RS17485 0.60 +0.8 417,548 - RALBFv3_RS17485 0.60 +0.5 417,710 - RALBFv3_RS17485 0.81 -0.5 417,713 - RALBFv3_RS17485 0.82 -0.6 417,713 - RALBFv3_RS17485 0.82 -0.1 417,885 - -1.5 417,885 - -0.7
Or see this region's nucleotide sequence