Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS15850 and RALBFv3_RS15855 are separated by 28 nucleotides RALBFv3_RS15855 and RALBFv3_RS15860 are separated by 110 nucleotides RALBFv3_RS15860 and RALBFv3_RS15865 are separated by 4 nucleotides RALBFv3_RS15865 and RALBFv3_RS15870 are separated by 337 nucleotides
RALBFv3_RS15850: RALBFv3_RS15850 - DegT/DnrJ/EryC1/StrS aminotransferase family protein, at 3,451,180 to 3,452,343
_RS15850
RALBFv3_RS15855: RALBFv3_RS15855 - 4-amino-4-deoxy-L-arabinose transferase, at 3,452,372 to 3,452,743
_RS15855
RALBFv3_RS15860: RALBFv3_RS15860 - UDP phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase, at 3,452,854 to 3,454,524
_RS15860
RALBFv3_RS15865: RALBFv3_RS15865 - hypothetical protein, at 3,454,529 to 3,454,909
_RS15865
RALBFv3_RS15870: RALBFv3_RS15870 - pyridoxal phosphate-dependent aminotransferase, at 3,455,247 to 3,456,488
_RS15870
Position (kb)
3452
3453
3454
3455 Strain fitness (log2 ratio)
-2
-1
0
1 at 3452.753 kb on - strand at 3452.753 kb on - strand at 3452.806 kb on - strand at 3452.806 kb on - strand at 3453.099 kb on + strand, within RALBFv3_RS15860 at 3453.395 kb on + strand, within RALBFv3_RS15860 at 3453.527 kb on + strand, within RALBFv3_RS15860 at 3454.591 kb on - strand, within RALBFv3_RS15865 at 3454.782 kb on - strand, within RALBFv3_RS15865 at 3455.078 kb on + strand at 3455.213 kb on + strand at 3455.213 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 3,452,753 - -0.1 3,452,753 - -0.5 3,452,806 - -0.7 3,452,806 - -0.3 3,453,099 + RALBFv3_RS15860 0.15 +1.0 3,453,395 + RALBFv3_RS15860 0.32 -0.0 3,453,527 + RALBFv3_RS15860 0.40 -0.0 3,454,591 - RALBFv3_RS15865 0.16 +1.0 3,454,782 - RALBFv3_RS15865 0.66 -0.6 3,455,078 + -1.8 3,455,213 + -0.4 3,455,213 + +0.2
Or see this region's nucleotide sequence