Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS15860

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS15850 and RALBFv3_RS15855 are separated by 28 nucleotidesRALBFv3_RS15855 and RALBFv3_RS15860 are separated by 110 nucleotidesRALBFv3_RS15860 and RALBFv3_RS15865 are separated by 4 nucleotidesRALBFv3_RS15865 and RALBFv3_RS15870 are separated by 337 nucleotides RALBFv3_RS15850: RALBFv3_RS15850 - DegT/DnrJ/EryC1/StrS aminotransferase family protein, at 3,451,180 to 3,452,343 _RS15850 RALBFv3_RS15855: RALBFv3_RS15855 - 4-amino-4-deoxy-L-arabinose transferase, at 3,452,372 to 3,452,743 _RS15855 RALBFv3_RS15860: RALBFv3_RS15860 - UDP phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase, at 3,452,854 to 3,454,524 _RS15860 RALBFv3_RS15865: RALBFv3_RS15865 - hypothetical protein, at 3,454,529 to 3,454,909 _RS15865 RALBFv3_RS15870: RALBFv3_RS15870 - pyridoxal phosphate-dependent aminotransferase, at 3,455,247 to 3,456,488 _RS15870 Position (kb) 3452 3453 3454 3455Strain fitness (log2 ratio) -2 -1 0 1at 3452.753 kb on - strandat 3452.753 kb on - strandat 3452.806 kb on - strandat 3452.806 kb on - strandat 3453.099 kb on + strand, within RALBFv3_RS15860at 3453.395 kb on + strand, within RALBFv3_RS15860at 3453.527 kb on + strand, within RALBFv3_RS15860at 3454.591 kb on - strand, within RALBFv3_RS15865at 3454.782 kb on - strand, within RALBFv3_RS15865at 3455.078 kb on + strandat 3455.213 kb on + strandat 3455.213 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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3,452,753 - -0.1
3,452,753 - -0.5
3,452,806 - -0.7
3,452,806 - -0.3
3,453,099 + RALBFv3_RS15860 0.15 +1.0
3,453,395 + RALBFv3_RS15860 0.32 -0.0
3,453,527 + RALBFv3_RS15860 0.40 -0.0
3,454,591 - RALBFv3_RS15865 0.16 +1.0
3,454,782 - RALBFv3_RS15865 0.66 -0.6
3,455,078 + -1.8
3,455,213 + -0.4
3,455,213 + +0.2

Or see this region's nucleotide sequence