Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS15600 and RALBFv3_RS15605 are separated by 180 nucleotides RALBFv3_RS15605 and RALBFv3_RS15610 are separated by 9 nucleotides RALBFv3_RS15610 and RALBFv3_RS15615 are separated by 115 nucleotides
RALBFv3_RS15600: RALBFv3_RS15600 - hypothetical protein, at 3,400,779 to 3,401,624
_RS15600
RALBFv3_RS15605: RALBFv3_RS15605 - copper-translocating P-type ATPase, at 3,401,805 to 3,404,345
_RS15605
RALBFv3_RS15610: RALBFv3_RS15610 - cbb3-type cytochrome oxidase assembly protein CcoS, at 3,404,355 to 3,404,528
_RS15610
RALBFv3_RS15615: RALBFv3_RS15615 - cytochrome-c oxidase, cbb3-type subunit I, at 3,404,644 to 3,406,101
_RS15615
Position (kb)
3401
3402
3403
3404
3405 Strain fitness (log2 ratio)
-1
0
1 at 3401.048 kb on - strand, within RALBFv3_RS15600 at 3401.378 kb on + strand, within RALBFv3_RS15600 at 3401.380 kb on + strand, within RALBFv3_RS15600 at 3401.381 kb on - strand, within RALBFv3_RS15600 at 3401.470 kb on + strand, within RALBFv3_RS15600 at 3401.471 kb on - strand, within RALBFv3_RS15600 at 3401.471 kb on - strand, within RALBFv3_RS15600 at 3401.603 kb on - strand at 3401.603 kb on - strand at 3401.657 kb on - strand at 3401.664 kb on + strand at 3401.664 kb on + strand at 3401.665 kb on - strand at 3401.790 kb on + strand at 3401.791 kb on - strand at 3401.804 kb on - strand at 3401.905 kb on - strand at 3402.036 kb on + strand at 3402.111 kb on + strand, within RALBFv3_RS15605 at 3402.112 kb on - strand, within RALBFv3_RS15605 at 3402.112 kb on - strand, within RALBFv3_RS15605 at 3402.271 kb on - strand, within RALBFv3_RS15605 at 3402.469 kb on - strand, within RALBFv3_RS15605 at 3402.483 kb on + strand, within RALBFv3_RS15605 at 3402.708 kb on + strand, within RALBFv3_RS15605 at 3403.038 kb on + strand, within RALBFv3_RS15605 at 3403.038 kb on + strand, within RALBFv3_RS15605 at 3403.625 kb on - strand, within RALBFv3_RS15605 at 3403.625 kb on - strand, within RALBFv3_RS15605 at 3403.765 kb on + strand, within RALBFv3_RS15605 at 3403.980 kb on + strand, within RALBFv3_RS15605 at 3404.367 kb on + strand at 3404.577 kb on - strand at 3404.615 kb on + strand at 3404.622 kb on - strand at 3404.813 kb on - strand, within RALBFv3_RS15615 at 3404.887 kb on + strand, within RALBFv3_RS15615 at 3404.890 kb on + strand, within RALBFv3_RS15615 at 3405.005 kb on - strand, within RALBFv3_RS15615 at 3405.022 kb on + strand, within RALBFv3_RS15615 at 3405.154 kb on + strand, within RALBFv3_RS15615 at 3405.223 kb on + strand, within RALBFv3_RS15615 at 3405.233 kb on - strand, within RALBFv3_RS15615 at 3405.233 kb on - strand, within RALBFv3_RS15615 at 3405.233 kb on - strand, within RALBFv3_RS15615 at 3405.233 kb on - strand, within RALBFv3_RS15615 at 3405.276 kb on + strand, within RALBFv3_RS15615 at 3405.322 kb on + strand, within RALBFv3_RS15615 at 3405.322 kb on + strand, within RALBFv3_RS15615 at 3405.322 kb on + strand, within RALBFv3_RS15615 at 3405.325 kb on + strand, within RALBFv3_RS15615
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 3,401,048 - RALBFv3_RS15600 0.32 -0.3 3,401,378 + RALBFv3_RS15600 0.71 -0.3 3,401,380 + RALBFv3_RS15600 0.71 -1.4 3,401,381 - RALBFv3_RS15600 0.71 -0.7 3,401,470 + RALBFv3_RS15600 0.82 -0.1 3,401,471 - RALBFv3_RS15600 0.82 -0.3 3,401,471 - RALBFv3_RS15600 0.82 -0.8 3,401,603 - -1.2 3,401,603 - +0.2 3,401,657 - -0.3 3,401,664 + -0.0 3,401,664 + -0.4 3,401,665 - -0.4 3,401,790 + -0.4 3,401,791 - -0.2 3,401,804 - -0.0 3,401,905 - -0.1 3,402,036 + +0.1 3,402,111 + RALBFv3_RS15605 0.12 +0.2 3,402,112 - RALBFv3_RS15605 0.12 +0.5 3,402,112 - RALBFv3_RS15605 0.12 -1.3 3,402,271 - RALBFv3_RS15605 0.18 -1.2 3,402,469 - RALBFv3_RS15605 0.26 -0.4 3,402,483 + RALBFv3_RS15605 0.27 -0.6 3,402,708 + RALBFv3_RS15605 0.36 -0.3 3,403,038 + RALBFv3_RS15605 0.49 -0.3 3,403,038 + RALBFv3_RS15605 0.49 +1.0 3,403,625 - RALBFv3_RS15605 0.72 +0.3 3,403,625 - RALBFv3_RS15605 0.72 -0.0 3,403,765 + RALBFv3_RS15605 0.77 -0.6 3,403,980 + RALBFv3_RS15605 0.86 +1.0 3,404,367 + -0.6 3,404,577 - +0.4 3,404,615 + +0.5 3,404,622 - -0.2 3,404,813 - RALBFv3_RS15615 0.12 -0.6 3,404,887 + RALBFv3_RS15615 0.17 -0.5 3,404,890 + RALBFv3_RS15615 0.17 -0.5 3,405,005 - RALBFv3_RS15615 0.25 -0.4 3,405,022 + RALBFv3_RS15615 0.26 +0.8 3,405,154 + RALBFv3_RS15615 0.35 -0.8 3,405,223 + RALBFv3_RS15615 0.40 +0.2 3,405,233 - RALBFv3_RS15615 0.40 -0.1 3,405,233 - RALBFv3_RS15615 0.40 -0.2 3,405,233 - RALBFv3_RS15615 0.40 +1.0 3,405,233 - RALBFv3_RS15615 0.40 -0.0 3,405,276 + RALBFv3_RS15615 0.43 +0.0 3,405,322 + RALBFv3_RS15615 0.47 -0.4 3,405,322 + RALBFv3_RS15615 0.47 +0.0 3,405,322 + RALBFv3_RS15615 0.47 +1.0 3,405,325 + RALBFv3_RS15615 0.47 -0.2
Or see this region's nucleotide sequence