Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS15505

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS15495 and RALBFv3_RS15500 are separated by 10 nucleotidesRALBFv3_RS15500 and RALBFv3_RS15505 are separated by 1 nucleotidesRALBFv3_RS15505 and RALBFv3_RS15510 are separated by 70 nucleotidesRALBFv3_RS15510 and RALBFv3_RS15515 are separated by 16 nucleotides RALBFv3_RS15495: RALBFv3_RS15495 - hypothetical protein, at 3,376,339 to 3,377,331 _RS15495 RALBFv3_RS15500: RALBFv3_RS15500 - polysaccharide deacetylase family protein, at 3,377,342 to 3,378,163 _RS15500 RALBFv3_RS15505: RALBFv3_RS15505 - sterol desaturase family protein, at 3,378,165 to 3,379,139 _RS15505 RALBFv3_RS15510: RALBFv3_RS15510 - membrane protein, at 3,379,210 to 3,380,070 _RS15510 RALBFv3_RS15515: RALBFv3_RS15515 - competence/damage-inducible protein A, at 3,380,087 to 3,380,905 _RS15515 Position (kb) 3378 3379 3380Strain fitness (log2 ratio) -2 -1 0 1 2at 3377.303 kb on - strandat 3377.303 kb on - strandat 3377.578 kb on + strand, within RALBFv3_RS15500at 3377.578 kb on + strand, within RALBFv3_RS15500at 3377.647 kb on + strand, within RALBFv3_RS15500at 3377.666 kb on + strand, within RALBFv3_RS15500at 3378.240 kb on + strandat 3378.781 kb on - strand, within RALBFv3_RS15505at 3378.781 kb on - strand, within RALBFv3_RS15505at 3379.176 kb on + strandat 3379.176 kb on + strandat 3379.555 kb on + strand, within RALBFv3_RS15510at 3379.721 kb on - strand, within RALBFv3_RS15510at 3379.921 kb on + strand, within RALBFv3_RS15510at 3379.922 kb on - strand, within RALBFv3_RS15510at 3380.068 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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3,377,303 - -0.8
3,377,303 - -0.3
3,377,578 + RALBFv3_RS15500 0.29 -0.0
3,377,578 + RALBFv3_RS15500 0.29 -0.4
3,377,647 + RALBFv3_RS15500 0.37 +2.6
3,377,666 + RALBFv3_RS15500 0.39 -0.7
3,378,240 + -1.8
3,378,781 - RALBFv3_RS15505 0.63 -0.7
3,378,781 - RALBFv3_RS15505 0.63 -2.0
3,379,176 + +0.0
3,379,176 + -0.8
3,379,555 + RALBFv3_RS15510 0.40 +1.0
3,379,721 - RALBFv3_RS15510 0.59 -0.7
3,379,921 + RALBFv3_RS15510 0.83 +0.2
3,379,922 - RALBFv3_RS15510 0.83 -0.5
3,380,068 + -1.0

Or see this region's nucleotide sequence