Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS15495 and RALBFv3_RS15500 are separated by 10 nucleotides RALBFv3_RS15500 and RALBFv3_RS15505 are separated by 1 nucleotides RALBFv3_RS15505 and RALBFv3_RS15510 are separated by 70 nucleotides RALBFv3_RS15510 and RALBFv3_RS15515 are separated by 16 nucleotides
RALBFv3_RS15495: RALBFv3_RS15495 - hypothetical protein, at 3,376,339 to 3,377,331
_RS15495
RALBFv3_RS15500: RALBFv3_RS15500 - polysaccharide deacetylase family protein, at 3,377,342 to 3,378,163
_RS15500
RALBFv3_RS15505: RALBFv3_RS15505 - sterol desaturase family protein, at 3,378,165 to 3,379,139
_RS15505
RALBFv3_RS15510: RALBFv3_RS15510 - membrane protein, at 3,379,210 to 3,380,070
_RS15510
RALBFv3_RS15515: RALBFv3_RS15515 - competence/damage-inducible protein A, at 3,380,087 to 3,380,905
_RS15515
Position (kb)
3378
3379
3380 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3377.303 kb on - strand at 3377.303 kb on - strand at 3377.578 kb on + strand, within RALBFv3_RS15500 at 3377.578 kb on + strand, within RALBFv3_RS15500 at 3377.647 kb on + strand, within RALBFv3_RS15500 at 3377.666 kb on + strand, within RALBFv3_RS15500 at 3378.240 kb on + strand at 3378.781 kb on - strand, within RALBFv3_RS15505 at 3378.781 kb on - strand, within RALBFv3_RS15505 at 3379.176 kb on + strand at 3379.176 kb on + strand at 3379.555 kb on + strand, within RALBFv3_RS15510 at 3379.721 kb on - strand, within RALBFv3_RS15510 at 3379.921 kb on + strand, within RALBFv3_RS15510 at 3379.922 kb on - strand, within RALBFv3_RS15510 at 3380.068 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 3,377,303 - -0.8 3,377,303 - -0.3 3,377,578 + RALBFv3_RS15500 0.29 -0.0 3,377,578 + RALBFv3_RS15500 0.29 -0.4 3,377,647 + RALBFv3_RS15500 0.37 +2.6 3,377,666 + RALBFv3_RS15500 0.39 -0.7 3,378,240 + -1.8 3,378,781 - RALBFv3_RS15505 0.63 -0.7 3,378,781 - RALBFv3_RS15505 0.63 -2.0 3,379,176 + +0.0 3,379,176 + -0.8 3,379,555 + RALBFv3_RS15510 0.40 +1.0 3,379,721 - RALBFv3_RS15510 0.59 -0.7 3,379,921 + RALBFv3_RS15510 0.83 +0.2 3,379,922 - RALBFv3_RS15510 0.83 -0.5 3,380,068 + -1.0
Or see this region's nucleotide sequence