Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS15490

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS15485 and RALBFv3_RS15490 are separated by 81 nucleotidesRALBFv3_RS15490 and RALBFv3_RS15495 are separated by 135 nucleotides RALBFv3_RS15485: RALBFv3_RS15485 - amino-acid N-acetyltransferase, at 3,370,729 to 3,372,126 _RS15485 RALBFv3_RS15490: RALBFv3_RS15490 - ATP-dependent RNA helicase HrpA, at 3,372,208 to 3,376,203 _RS15490 RALBFv3_RS15495: RALBFv3_RS15495 - hypothetical protein, at 3,376,339 to 3,377,331 _RS15495 Position (kb) 3372 3373 3374 3375 3376 3377Strain fitness (log2 ratio) -2 -1 0 1at 3371.309 kb on + strand, within RALBFv3_RS15485at 3371.310 kb on - strand, within RALBFv3_RS15485at 3371.313 kb on - strand, within RALBFv3_RS15485at 3371.901 kb on + strand, within RALBFv3_RS15485at 3371.990 kb on + strandat 3371.990 kb on + strandat 3371.991 kb on - strandat 3371.991 kb on - strandat 3371.991 kb on - strandat 3373.306 kb on + strand, within RALBFv3_RS15490at 3373.342 kb on + strand, within RALBFv3_RS15490at 3374.066 kb on - strand, within RALBFv3_RS15490at 3374.152 kb on + strand, within RALBFv3_RS15490at 3374.395 kb on + strand, within RALBFv3_RS15490at 3374.672 kb on - strand, within RALBFv3_RS15490at 3375.554 kb on - strand, within RALBFv3_RS15490at 3376.338 kb on + strandat 3376.339 kb on - strandat 3376.943 kb on - strand, within RALBFv3_RS15495

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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3,371,309 + RALBFv3_RS15485 0.41 -1.5
3,371,310 - RALBFv3_RS15485 0.42 -2.3
3,371,313 - RALBFv3_RS15485 0.42 +1.3
3,371,901 + RALBFv3_RS15485 0.84 +0.4
3,371,990 + -1.7
3,371,990 + +0.4
3,371,991 - -0.0
3,371,991 - +0.5
3,371,991 - -0.1
3,373,306 + RALBFv3_RS15490 0.27 -1.5
3,373,342 + RALBFv3_RS15490 0.28 -1.7
3,374,066 - RALBFv3_RS15490 0.46 -1.5
3,374,152 + RALBFv3_RS15490 0.49 -1.1
3,374,395 + RALBFv3_RS15490 0.55 -0.8
3,374,672 - RALBFv3_RS15490 0.62 -2.3
3,375,554 - RALBFv3_RS15490 0.84 -1.4
3,376,338 + -0.6
3,376,339 - -0.8
3,376,943 - RALBFv3_RS15495 0.61 -1.9

Or see this region's nucleotide sequence