Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS15390

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS15380 and RALBFv3_RS15385 are separated by 85 nucleotidesRALBFv3_RS15385 and RALBFv3_RS15390 are separated by 111 nucleotidesRALBFv3_RS15390 and RALBFv3_RS15395 are separated by 35 nucleotides RALBFv3_RS15380: RALBFv3_RS15380 - methyl-accepting chemotaxis protein, at 3,348,652 to 3,350,190 _RS15380 RALBFv3_RS15385: RALBFv3_RS15385 - oxidative damage protection protein, at 3,350,276 to 3,350,551 _RS15385 RALBFv3_RS15390: RALBFv3_RS15390 - oxidoreductase, at 3,350,663 to 3,351,736 _RS15390 RALBFv3_RS15395: RALBFv3_RS15395 - MurR/RpiR family transcriptional regulator, at 3,351,772 to 3,352,689 _RS15395 Position (kb) 3350 3351 3352Strain fitness (log2 ratio) -1 0 1at 3350.299 kb on - strandat 3351.021 kb on + strand, within RALBFv3_RS15390at 3351.561 kb on + strand, within RALBFv3_RS15390at 3351.562 kb on - strand, within RALBFv3_RS15390at 3352.014 kb on - strand, within RALBFv3_RS15395at 3352.014 kb on - strand, within RALBFv3_RS15395at 3352.039 kb on + strand, within RALBFv3_RS15395at 3352.039 kb on + strand, within RALBFv3_RS15395at 3352.039 kb on + strand, within RALBFv3_RS15395at 3352.133 kb on + strand, within RALBFv3_RS15395at 3352.533 kb on + strand, within RALBFv3_RS15395at 3352.536 kb on - strand, within RALBFv3_RS15395at 3352.692 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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3,350,299 - -0.2
3,351,021 + RALBFv3_RS15390 0.33 -1.3
3,351,561 + RALBFv3_RS15390 0.84 +1.0
3,351,562 - RALBFv3_RS15390 0.84 +1.0
3,352,014 - RALBFv3_RS15395 0.26 -0.5
3,352,014 - RALBFv3_RS15395 0.26 +1.0
3,352,039 + RALBFv3_RS15395 0.29 +0.2
3,352,039 + RALBFv3_RS15395 0.29 -0.1
3,352,039 + RALBFv3_RS15395 0.29 -0.3
3,352,133 + RALBFv3_RS15395 0.39 -0.6
3,352,533 + RALBFv3_RS15395 0.83 -0.0
3,352,536 - RALBFv3_RS15395 0.83 -0.1
3,352,692 + +1.0

Or see this region's nucleotide sequence