Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS15230

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS15220 and RALBFv3_RS15225 are separated by 15 nucleotidesRALBFv3_RS15225 and RALBFv3_RS15230 are separated by 1 nucleotidesRALBFv3_RS15230 and RALBFv3_RS15235 are separated by 8 nucleotides RALBFv3_RS15220: RALBFv3_RS15220 - 5'/3'-nucleotidase SurE, at 3,314,802 to 3,315,557 _RS15220 RALBFv3_RS15225: RALBFv3_RS15225 - protein-L-isoaspartate(D-aspartate) O-methyltransferase, at 3,315,573 to 3,316,499 _RS15225 RALBFv3_RS15230: RALBFv3_RS15230 - hypothetical protein, at 3,316,501 to 3,317,328 _RS15230 RALBFv3_RS15235: RALBFv3_RS15235 - RNA polymerase sigma factor RpoS, at 3,317,337 to 3,318,467 _RS15235 Position (kb) 3316 3317 3318Strain fitness (log2 ratio) -1 0 1at 3316.158 kb on + strand, within RALBFv3_RS15225at 3316.158 kb on + strand, within RALBFv3_RS15225at 3316.159 kb on - strand, within RALBFv3_RS15225at 3316.159 kb on - strand, within RALBFv3_RS15225at 3316.498 kb on - strandat 3316.717 kb on + strand, within RALBFv3_RS15230at 3316.860 kb on + strand, within RALBFv3_RS15230at 3317.148 kb on + strandat 3317.908 kb on - strand, within RALBFv3_RS15235at 3317.908 kb on - strand, within RALBFv3_RS15235

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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3,316,158 + RALBFv3_RS15225 0.63 -1.2
3,316,158 + RALBFv3_RS15225 0.63 +0.0
3,316,159 - RALBFv3_RS15225 0.63 -0.3
3,316,159 - RALBFv3_RS15225 0.63 +0.4
3,316,498 - -1.0
3,316,717 + RALBFv3_RS15230 0.26 -0.0
3,316,860 + RALBFv3_RS15230 0.43 -1.3
3,317,148 + +1.0
3,317,908 - RALBFv3_RS15235 0.50 -0.8
3,317,908 - RALBFv3_RS15235 0.50 -1.3

Or see this region's nucleotide sequence