Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS15155 and RALBFv3_RS15160 are separated by 90 nucleotides RALBFv3_RS15160 and RALBFv3_RS15165 are separated by 23 nucleotides RALBFv3_RS15165 and RALBFv3_RS15170 overlap by 1 nucleotides RALBFv3_RS15170 and RALBFv3_RS15175 are separated by 36 nucleotides
RALBFv3_RS15155: RALBFv3_RS15155 - DNA polymerase III subunit gamma/tau, at 3,301,415 to 3,303,625
_RS15155
RALBFv3_RS15160: RALBFv3_RS15160 - nucleoid-associated protein, YbaB/EbfC family, at 3,303,716 to 3,304,063
_RS15160
RALBFv3_RS15165: RALBFv3_RS15165 - antibiotic biosynthesis monooxygenase, at 3,304,087 to 3,304,386
_RS15165
RALBFv3_RS15170: RALBFv3_RS15170 - recombination protein RecR, at 3,304,386 to 3,305,006
_RS15170
RALBFv3_RS15175: RALBFv3_RS15175 - carbohydrate porin, at 3,305,043 to 3,306,437
_RS15175
Position (kb) 3304
3305 Strain fitness (log2 ratio) -1
0
1
2 at 3304.301 kb on - strand, within RALBFv3_RS15165 at 3304.486 kb on - strand, within RALBFv3_RS15170 at 3304.486 kb on - strand, within RALBFv3_RS15170 at 3304.486 kb on - strand, within RALBFv3_RS15170 at 3304.696 kb on - strand, within RALBFv3_RS15170 at 3304.953 kb on + strand at 3305.119 kb on + strand at 3305.167 kb on + strand at 3305.167 kb on + strand at 3305.168 kb on - strand at 3305.168 kb on - strand at 3305.170 kb on + strand at 3305.171 kb on - strand at 3305.197 kb on + strand, within RALBFv3_RS15175 at 3305.198 kb on - strand, within RALBFv3_RS15175 at 3305.198 kb on - strand, within RALBFv3_RS15175 at 3305.377 kb on + strand, within RALBFv3_RS15175
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 3,304,301 - RALBFv3_RS15165 0.71 -0.9 3,304,486 - RALBFv3_RS15170 0.16 -0.2 3,304,486 - RALBFv3_RS15170 0.16 -0.9 3,304,486 - RALBFv3_RS15170 0.16 +1.0 3,304,696 - RALBFv3_RS15170 0.50 -0.6 3,304,953 + +0.1 3,305,119 + +0.2 3,305,167 + -0.7 3,305,167 + -0.4 3,305,168 - -0.3 3,305,168 - -0.7 3,305,170 + +0.2 3,305,171 - -0.8 3,305,197 + RALBFv3_RS15175 0.11 +0.4 3,305,198 - RALBFv3_RS15175 0.11 +0.4 3,305,198 - RALBFv3_RS15175 0.11 +1.0 3,305,377 + RALBFv3_RS15175 0.24 +2.0
Or see this region's nucleotide sequence