Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS14455

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS14445 and RALBFv3_RS14450 are separated by 110 nucleotidesRALBFv3_RS14450 and RALBFv3_RS14455 are separated by 11 nucleotidesRALBFv3_RS14455 and RALBFv3_RS14460 are separated by 97 nucleotides RALBFv3_RS14445: RALBFv3_RS14445 - RNA polymerase sigma factor RpoE, at 3,156,598 to 3,157,197 _RS14445 RALBFv3_RS14450: RALBFv3_RS14450 - anti-sigma factor, at 3,157,308 to 3,157,940 _RS14450 RALBFv3_RS14455: RALBFv3_RS14455 - sugar dehydratase, at 3,157,952 to 3,159,049 _RS14455 RALBFv3_RS14460: RALBFv3_RS14460 - peptidase, at 3,159,147 to 3,160,664 _RS14460 Position (kb) 3157 3158 3159 3160Strain fitness (log2 ratio) -2 -1 0 1at 3157.042 kb on - strand, within RALBFv3_RS14445at 3157.069 kb on + strand, within RALBFv3_RS14445at 3157.207 kb on - strandat 3157.250 kb on - strandat 3158.228 kb on + strand, within RALBFv3_RS14455at 3158.521 kb on - strand, within RALBFv3_RS14455at 3159.152 kb on + strandat 3159.270 kb on + strandat 3159.271 kb on - strandat 3159.271 kb on - strandat 3159.573 kb on + strand, within RALBFv3_RS14460at 3159.574 kb on - strand, within RALBFv3_RS14460at 3159.924 kb on + strand, within RALBFv3_RS14460at 3159.925 kb on - strand, within RALBFv3_RS14460

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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3,157,042 - RALBFv3_RS14445 0.74 -1.4
3,157,069 + RALBFv3_RS14445 0.79 -2.6
3,157,207 - -1.3
3,157,250 - -0.9
3,158,228 + RALBFv3_RS14455 0.25 -1.6
3,158,521 - RALBFv3_RS14455 0.52 -1.6
3,159,152 + +1.0
3,159,270 + -0.9
3,159,271 - +1.0
3,159,271 - -1.6
3,159,573 + RALBFv3_RS14460 0.28 -0.8
3,159,574 - RALBFv3_RS14460 0.28 -1.3
3,159,924 + RALBFv3_RS14460 0.51 -1.7
3,159,925 - RALBFv3_RS14460 0.51 +1.0

Or see this region's nucleotide sequence