Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS14445

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS14440 and RALBFv3_RS24060 overlap by 1 nucleotidesRALBFv3_RS24060 and RALBFv3_RS14445 are separated by 79 nucleotidesRALBFv3_RS14445 and RALBFv3_RS14450 are separated by 110 nucleotidesRALBFv3_RS14450 and RALBFv3_RS14455 are separated by 11 nucleotides RALBFv3_RS14440: RALBFv3_RS14440 - beta-ketoacyl-[acyl-carrier-protein] synthase II, at 3,154,756 to 3,155,988 _RS14440 RALBFv3_RS24060: RALBFv3_RS24060 - hypothetical protein, at 3,155,988 to 3,156,518 _RS24060 RALBFv3_RS14445: RALBFv3_RS14445 - RNA polymerase sigma factor RpoE, at 3,156,598 to 3,157,197 _RS14445 RALBFv3_RS14450: RALBFv3_RS14450 - anti-sigma factor, at 3,157,308 to 3,157,940 _RS14450 RALBFv3_RS14455: RALBFv3_RS14455 - sugar dehydratase, at 3,157,952 to 3,159,049 _RS14455 Position (kb) 3156 3157 3158Strain fitness (log2 ratio) -2 -1 0 1at 3156.359 kb on - strand, within RALBFv3_RS24060at 3156.577 kb on + strandat 3156.578 kb on - strandat 3156.683 kb on - strand, within RALBFv3_RS14445at 3157.042 kb on - strand, within RALBFv3_RS14445at 3157.069 kb on + strand, within RALBFv3_RS14445at 3157.207 kb on - strandat 3157.250 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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3,156,359 - RALBFv3_RS24060 0.70 +1.0
3,156,577 + -1.2
3,156,578 - -1.3
3,156,683 - RALBFv3_RS14445 0.14 -1.8
3,157,042 - RALBFv3_RS14445 0.74 -1.4
3,157,069 + RALBFv3_RS14445 0.79 -2.6
3,157,207 - -1.3
3,157,250 - -0.9

Or see this region's nucleotide sequence