Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS14440 and RALBFv3_RS24060 overlap by 1 nucleotides RALBFv3_RS24060 and RALBFv3_RS14445 are separated by 79 nucleotides RALBFv3_RS14445 and RALBFv3_RS14450 are separated by 110 nucleotides RALBFv3_RS14450 and RALBFv3_RS14455 are separated by 11 nucleotides
RALBFv3_RS14440: RALBFv3_RS14440 - beta-ketoacyl-[acyl-carrier-protein] synthase II, at 3,154,756 to 3,155,988
_RS14440
RALBFv3_RS24060: RALBFv3_RS24060 - hypothetical protein, at 3,155,988 to 3,156,518
_RS24060
RALBFv3_RS14445: RALBFv3_RS14445 - RNA polymerase sigma factor RpoE, at 3,156,598 to 3,157,197
_RS14445
RALBFv3_RS14450: RALBFv3_RS14450 - anti-sigma factor, at 3,157,308 to 3,157,940
_RS14450
RALBFv3_RS14455: RALBFv3_RS14455 - sugar dehydratase, at 3,157,952 to 3,159,049
_RS14455
Position (kb)
3156
3157
3158 Strain fitness (log2 ratio)
-2
-1
0
1 at 3156.359 kb on - strand, within RALBFv3_RS24060 at 3156.577 kb on + strand at 3156.578 kb on - strand at 3156.683 kb on - strand, within RALBFv3_RS14445 at 3157.042 kb on - strand, within RALBFv3_RS14445 at 3157.069 kb on + strand, within RALBFv3_RS14445 at 3157.207 kb on - strand at 3157.250 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 3,156,359 - RALBFv3_RS24060 0.70 +1.0 3,156,577 + -1.2 3,156,578 - -1.3 3,156,683 - RALBFv3_RS14445 0.14 -1.8 3,157,042 - RALBFv3_RS14445 0.74 -1.4 3,157,069 + RALBFv3_RS14445 0.79 -2.6 3,157,207 - -1.3 3,157,250 - -0.9
Or see this region's nucleotide sequence