Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS14390 and RALBFv3_RS14395 are separated by 17 nucleotides RALBFv3_RS14395 and RALBFv3_RS14400 are separated by 3 nucleotides RALBFv3_RS14400 and RALBFv3_RS14405 are separated by 201 nucleotides RALBFv3_RS14405 and RALBFv3_RS14410 are separated by 115 nucleotides
RALBFv3_RS14390: RALBFv3_RS14390 - S49 family peptidase, at 3,146,683 to 3,147,819
_RS14390
RALBFv3_RS14395: RALBFv3_RS14395 - S-adenosylmethionine-dependent methyltransferase, at 3,147,837 to 3,148,568
_RS14395
RALBFv3_RS14400: RALBFv3_RS14400 - septum formation protein Maf, at 3,148,572 to 3,149,168
_RS14400
RALBFv3_RS14405: RALBFv3_RS14405 - metal-binding protein, at 3,149,370 to 3,149,909
_RS14405
RALBFv3_RS14410: RALBFv3_RS14410 - 50S ribosomal protein L32, at 3,150,025 to 3,150,204
_RS14410
Position (kb)
3148
3149
3150 Strain fitness (log2 ratio)
-1
0
1
2 at 3147.818 kb on - strand at 3147.818 kb on - strand at 3148.553 kb on - strand at 3148.589 kb on - strand at 3148.589 kb on - strand at 3148.599 kb on - strand at 3148.737 kb on + strand, within RALBFv3_RS14400 at 3148.828 kb on + strand, within RALBFv3_RS14400 at 3148.859 kb on + strand, within RALBFv3_RS14400 at 3148.859 kb on + strand, within RALBFv3_RS14400 at 3149.169 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 3,147,818 - +0.1 3,147,818 - +0.4 3,148,553 - -0.5 3,148,589 - -1.3 3,148,589 - +1.0 3,148,599 - -0.2 3,148,737 + RALBFv3_RS14400 0.28 -0.6 3,148,828 + RALBFv3_RS14400 0.43 -0.9 3,148,859 + RALBFv3_RS14400 0.48 -1.3 3,148,859 + RALBFv3_RS14400 0.48 +2.0 3,149,169 - +0.8
Or see this region's nucleotide sequence