Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS14400

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS14390 and RALBFv3_RS14395 are separated by 17 nucleotidesRALBFv3_RS14395 and RALBFv3_RS14400 are separated by 3 nucleotidesRALBFv3_RS14400 and RALBFv3_RS14405 are separated by 201 nucleotidesRALBFv3_RS14405 and RALBFv3_RS14410 are separated by 115 nucleotides RALBFv3_RS14390: RALBFv3_RS14390 - S49 family peptidase, at 3,146,683 to 3,147,819 _RS14390 RALBFv3_RS14395: RALBFv3_RS14395 - S-adenosylmethionine-dependent methyltransferase, at 3,147,837 to 3,148,568 _RS14395 RALBFv3_RS14400: RALBFv3_RS14400 - septum formation protein Maf, at 3,148,572 to 3,149,168 _RS14400 RALBFv3_RS14405: RALBFv3_RS14405 - metal-binding protein, at 3,149,370 to 3,149,909 _RS14405 RALBFv3_RS14410: RALBFv3_RS14410 - 50S ribosomal protein L32, at 3,150,025 to 3,150,204 _RS14410 Position (kb) 3148 3149 3150Strain fitness (log2 ratio) -1 0 1 2at 3147.818 kb on - strandat 3147.818 kb on - strandat 3148.553 kb on - strandat 3148.589 kb on - strandat 3148.589 kb on - strandat 3148.599 kb on - strandat 3148.737 kb on + strand, within RALBFv3_RS14400at 3148.828 kb on + strand, within RALBFv3_RS14400at 3148.859 kb on + strand, within RALBFv3_RS14400at 3148.859 kb on + strand, within RALBFv3_RS14400at 3149.169 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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3,147,818 - +0.1
3,147,818 - +0.4
3,148,553 - -0.5
3,148,589 - -1.3
3,148,589 - +1.0
3,148,599 - -0.2
3,148,737 + RALBFv3_RS14400 0.28 -0.6
3,148,828 + RALBFv3_RS14400 0.43 -0.9
3,148,859 + RALBFv3_RS14400 0.48 -1.3
3,148,859 + RALBFv3_RS14400 0.48 +2.0
3,149,169 - +0.8

Or see this region's nucleotide sequence