Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS14375

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS14370 and RALBFv3_RS14375 are separated by 715 nucleotidesRALBFv3_RS14375 and RALBFv3_RS14380 overlap by 20 nucleotidesRALBFv3_RS14380 and RALBFv3_RS14385 overlap by 8 nucleotides RALBFv3_RS14370: RALBFv3_RS14370 - ribonuclease E/G, at 3,140,788 to 3,143,829 _RS14370 RALBFv3_RS14375: RALBFv3_RS14375 - RluA family pseudouridine synthase, at 3,144,545 to 3,145,597 _RS14375 RALBFv3_RS14380: RALBFv3_RS14380 - haloacid dehalogenase, at 3,145,578 to 3,146,237 _RS14380 RALBFv3_RS14385: RALBFv3_RS14385 - Rieske (2Fe-2S) protein, at 3,146,230 to 3,146,646 _RS14385 Position (kb) 3144 3145 3146Strain fitness (log2 ratio) -2 -1 0 1 2at 3144.469 kb on - strandat 3144.473 kb on + strandat 3144.474 kb on - strandat 3144.555 kb on + strandat 3144.555 kb on + strandat 3144.635 kb on + strandat 3144.635 kb on + strandat 3144.722 kb on + strand, within RALBFv3_RS14375at 3144.723 kb on - strand, within RALBFv3_RS14375at 3144.902 kb on - strand, within RALBFv3_RS14375at 3144.902 kb on - strand, within RALBFv3_RS14375at 3145.289 kb on + strand, within RALBFv3_RS14375at 3145.290 kb on - strand, within RALBFv3_RS14375at 3145.290 kb on - strand, within RALBFv3_RS14375at 3145.291 kb on + strand, within RALBFv3_RS14375at 3145.291 kb on + strand, within RALBFv3_RS14375at 3145.291 kb on + strand, within RALBFv3_RS14375at 3145.292 kb on - strand, within RALBFv3_RS14375at 3145.292 kb on - strand, within RALBFv3_RS14375at 3145.385 kb on + strand, within RALBFv3_RS14375at 3145.782 kb on + strand, within RALBFv3_RS14380at 3145.815 kb on + strand, within RALBFv3_RS14380at 3145.815 kb on + strand, within RALBFv3_RS14380at 3145.890 kb on + strand, within RALBFv3_RS14380at 3145.890 kb on + strand, within RALBFv3_RS14380at 3146.455 kb on + strand, within RALBFv3_RS14385at 3146.456 kb on - strand, within RALBFv3_RS14385at 3146.485 kb on + strand, within RALBFv3_RS14385

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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3,144,469 - +1.0
3,144,473 + +1.0
3,144,474 - +0.0
3,144,555 + +1.0
3,144,555 + -0.1
3,144,635 + +2.0
3,144,635 + +0.5
3,144,722 + RALBFv3_RS14375 0.17 -2.2
3,144,723 - RALBFv3_RS14375 0.17 -1.1
3,144,902 - RALBFv3_RS14375 0.34 -0.2
3,144,902 - RALBFv3_RS14375 0.34 -1.0
3,145,289 + RALBFv3_RS14375 0.71 -0.2
3,145,290 - RALBFv3_RS14375 0.71 -0.7
3,145,290 - RALBFv3_RS14375 0.71 -0.9
3,145,291 + RALBFv3_RS14375 0.71 -0.4
3,145,291 + RALBFv3_RS14375 0.71 -0.2
3,145,291 + RALBFv3_RS14375 0.71 -0.6
3,145,292 - RALBFv3_RS14375 0.71 -0.7
3,145,292 - RALBFv3_RS14375 0.71 -0.6
3,145,385 + RALBFv3_RS14375 0.80 -0.0
3,145,782 + RALBFv3_RS14380 0.31 -0.2
3,145,815 + RALBFv3_RS14380 0.36 -0.3
3,145,815 + RALBFv3_RS14380 0.36 -1.5
3,145,890 + RALBFv3_RS14380 0.47 -0.1
3,145,890 + RALBFv3_RS14380 0.47 +0.0
3,146,455 + RALBFv3_RS14385 0.54 -0.2
3,146,456 - RALBFv3_RS14385 0.54 -0.2
3,146,485 + RALBFv3_RS14385 0.61 +0.0

Or see this region's nucleotide sequence