Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS14370 and RALBFv3_RS14375 are separated by 715 nucleotides RALBFv3_RS14375 and RALBFv3_RS14380 overlap by 20 nucleotides RALBFv3_RS14380 and RALBFv3_RS14385 overlap by 8 nucleotides
RALBFv3_RS14370: RALBFv3_RS14370 - ribonuclease E/G, at 3,140,788 to 3,143,829
_RS14370
RALBFv3_RS14375: RALBFv3_RS14375 - RluA family pseudouridine synthase, at 3,144,545 to 3,145,597
_RS14375
RALBFv3_RS14380: RALBFv3_RS14380 - haloacid dehalogenase, at 3,145,578 to 3,146,237
_RS14380
RALBFv3_RS14385: RALBFv3_RS14385 - Rieske (2Fe-2S) protein, at 3,146,230 to 3,146,646
_RS14385
Position (kb)
3144
3145
3146 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3144.469 kb on - strand at 3144.473 kb on + strand at 3144.474 kb on - strand at 3144.555 kb on + strand at 3144.555 kb on + strand at 3144.635 kb on + strand at 3144.635 kb on + strand at 3144.722 kb on + strand, within RALBFv3_RS14375 at 3144.723 kb on - strand, within RALBFv3_RS14375 at 3144.902 kb on - strand, within RALBFv3_RS14375 at 3144.902 kb on - strand, within RALBFv3_RS14375 at 3145.289 kb on + strand, within RALBFv3_RS14375 at 3145.290 kb on - strand, within RALBFv3_RS14375 at 3145.290 kb on - strand, within RALBFv3_RS14375 at 3145.291 kb on + strand, within RALBFv3_RS14375 at 3145.291 kb on + strand, within RALBFv3_RS14375 at 3145.291 kb on + strand, within RALBFv3_RS14375 at 3145.292 kb on - strand, within RALBFv3_RS14375 at 3145.292 kb on - strand, within RALBFv3_RS14375 at 3145.385 kb on + strand, within RALBFv3_RS14375 at 3145.782 kb on + strand, within RALBFv3_RS14380 at 3145.815 kb on + strand, within RALBFv3_RS14380 at 3145.815 kb on + strand, within RALBFv3_RS14380 at 3145.890 kb on + strand, within RALBFv3_RS14380 at 3145.890 kb on + strand, within RALBFv3_RS14380 at 3146.455 kb on + strand, within RALBFv3_RS14385 at 3146.456 kb on - strand, within RALBFv3_RS14385 at 3146.485 kb on + strand, within RALBFv3_RS14385
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 3,144,469 - +1.0 3,144,473 + +1.0 3,144,474 - +0.0 3,144,555 + +1.0 3,144,555 + -0.1 3,144,635 + +2.0 3,144,635 + +0.5 3,144,722 + RALBFv3_RS14375 0.17 -2.2 3,144,723 - RALBFv3_RS14375 0.17 -1.1 3,144,902 - RALBFv3_RS14375 0.34 -0.2 3,144,902 - RALBFv3_RS14375 0.34 -1.0 3,145,289 + RALBFv3_RS14375 0.71 -0.2 3,145,290 - RALBFv3_RS14375 0.71 -0.7 3,145,290 - RALBFv3_RS14375 0.71 -0.9 3,145,291 + RALBFv3_RS14375 0.71 -0.4 3,145,291 + RALBFv3_RS14375 0.71 -0.2 3,145,291 + RALBFv3_RS14375 0.71 -0.6 3,145,292 - RALBFv3_RS14375 0.71 -0.7 3,145,292 - RALBFv3_RS14375 0.71 -0.6 3,145,385 + RALBFv3_RS14375 0.80 -0.0 3,145,782 + RALBFv3_RS14380 0.31 -0.2 3,145,815 + RALBFv3_RS14380 0.36 -0.3 3,145,815 + RALBFv3_RS14380 0.36 -1.5 3,145,890 + RALBFv3_RS14380 0.47 -0.1 3,145,890 + RALBFv3_RS14380 0.47 +0.0 3,146,455 + RALBFv3_RS14385 0.54 -0.2 3,146,456 - RALBFv3_RS14385 0.54 -0.2 3,146,485 + RALBFv3_RS14385 0.61 +0.0
Or see this region's nucleotide sequence