Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS14015 and RALBFv3_RS14020 are separated by 140 nucleotides RALBFv3_RS14020 and RALBFv3_RS14025 are separated by 147 nucleotides RALBFv3_RS14025 and RALBFv3_RS14030 are separated by 17 nucleotides
RALBFv3_RS14015: RALBFv3_RS14015 - voltage-gated chloride channel, at 3,063,556 to 3,065,046
_RS14015
RALBFv3_RS14020: RALBFv3_RS14020 - hypothetical protein, at 3,065,187 to 3,065,414
_RS14020
RALBFv3_RS14025: RALBFv3_RS14025 - hypothetical protein, at 3,065,562 to 3,066,530
_RS14025
RALBFv3_RS14030: RALBFv3_RS14030 - amino acid ABC transporter substrate-binding protein, at 3,066,548 to 3,067,459
_RS14030
Position (kb)
3065
3066
3067 Strain fitness (log2 ratio)
-2
-1
0
1 at 3065.060 kb on - strand at 3065.121 kb on - strand at 3065.121 kb on - strand at 3065.121 kb on - strand at 3065.124 kb on + strand at 3065.125 kb on + strand at 3065.157 kb on + strand at 3065.157 kb on + strand at 3065.158 kb on - strand at 3065.158 kb on - strand at 3065.165 kb on + strand at 3065.166 kb on - strand at 3065.166 kb on - strand at 3065.186 kb on + strand at 3065.187 kb on - strand at 3065.187 kb on - strand at 3065.198 kb on + strand at 3065.199 kb on - strand at 3065.199 kb on - strand at 3065.199 kb on - strand at 3065.264 kb on + strand, within RALBFv3_RS14020 at 3065.298 kb on + strand, within RALBFv3_RS14020 at 3065.298 kb on + strand, within RALBFv3_RS14020 at 3065.298 kb on + strand, within RALBFv3_RS14020 at 3065.298 kb on + strand, within RALBFv3_RS14020 at 3065.299 kb on - strand, within RALBFv3_RS14020 at 3065.299 kb on - strand, within RALBFv3_RS14020 at 3065.412 kb on + strand at 3065.412 kb on + strand at 3065.413 kb on - strand at 3065.413 kb on - strand at 3065.413 kb on - strand at 3065.522 kb on - strand at 3065.545 kb on - strand at 3065.717 kb on + strand, within RALBFv3_RS14025 at 3065.961 kb on + strand, within RALBFv3_RS14025 at 3065.961 kb on + strand, within RALBFv3_RS14025 at 3065.962 kb on - strand, within RALBFv3_RS14025 at 3065.962 kb on - strand, within RALBFv3_RS14025 at 3065.962 kb on - strand, within RALBFv3_RS14025 at 3065.962 kb on - strand, within RALBFv3_RS14025 at 3065.962 kb on - strand, within RALBFv3_RS14025 at 3065.962 kb on - strand, within RALBFv3_RS14025 at 3066.240 kb on - strand, within RALBFv3_RS14025 at 3066.436 kb on - strand at 3066.660 kb on + strand, within RALBFv3_RS14030 at 3066.776 kb on - strand, within RALBFv3_RS14030 at 3066.793 kb on - strand, within RALBFv3_RS14030 at 3066.793 kb on - strand, within RALBFv3_RS14030 at 3066.793 kb on - strand, within RALBFv3_RS14030 at 3067.065 kb on + strand, within RALBFv3_RS14030 at 3067.065 kb on + strand, within RALBFv3_RS14030 at 3067.065 kb on + strand, within RALBFv3_RS14030 at 3067.188 kb on + strand, within RALBFv3_RS14030 at 3067.188 kb on + strand, within RALBFv3_RS14030 at 3067.188 kb on + strand, within RALBFv3_RS14030 at 3067.188 kb on + strand, within RALBFv3_RS14030 at 3067.189 kb on - strand, within RALBFv3_RS14030 at 3067.189 kb on - strand, within RALBFv3_RS14030 at 3067.189 kb on - strand, within RALBFv3_RS14030 at 3067.189 kb on - strand, within RALBFv3_RS14030 at 3067.189 kb on - strand, within RALBFv3_RS14030 at 3067.293 kb on + strand, within RALBFv3_RS14030 at 3067.294 kb on - strand, within RALBFv3_RS14030 at 3067.477 kb on + strand at 3067.498 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 3,065,060 - -0.1 3,065,121 - -0.2 3,065,121 - -0.4 3,065,121 - -0.6 3,065,124 + -0.2 3,065,125 + +0.6 3,065,157 + -0.7 3,065,157 + -0.3 3,065,158 - -0.3 3,065,158 - +0.3 3,065,165 + +1.0 3,065,166 - +1.0 3,065,166 - -0.3 3,065,186 + -0.4 3,065,187 - +1.0 3,065,187 - +0.5 3,065,198 + +0.1 3,065,199 - -0.5 3,065,199 - +0.4 3,065,199 - -0.1 3,065,264 + RALBFv3_RS14020 0.34 +0.4 3,065,298 + RALBFv3_RS14020 0.49 -0.9 3,065,298 + RALBFv3_RS14020 0.49 -0.3 3,065,298 + RALBFv3_RS14020 0.49 +0.2 3,065,298 + RALBFv3_RS14020 0.49 +0.1 3,065,299 - RALBFv3_RS14020 0.49 -0.4 3,065,299 - RALBFv3_RS14020 0.49 +1.0 3,065,412 + -0.6 3,065,412 + -0.0 3,065,413 - -0.3 3,065,413 - +0.1 3,065,413 - +0.5 3,065,522 - -1.3 3,065,545 - +0.8 3,065,717 + RALBFv3_RS14025 0.16 -0.7 3,065,961 + RALBFv3_RS14025 0.41 -0.7 3,065,961 + RALBFv3_RS14025 0.41 -2.2 3,065,962 - RALBFv3_RS14025 0.41 +0.4 3,065,962 - RALBFv3_RS14025 0.41 -2.2 3,065,962 - RALBFv3_RS14025 0.41 -0.6 3,065,962 - RALBFv3_RS14025 0.41 -1.8 3,065,962 - RALBFv3_RS14025 0.41 +1.0 3,065,962 - RALBFv3_RS14025 0.41 -1.1 3,066,240 - RALBFv3_RS14025 0.70 -0.3 3,066,436 - +0.5 3,066,660 + RALBFv3_RS14030 0.12 -0.0 3,066,776 - RALBFv3_RS14030 0.25 -0.1 3,066,793 - RALBFv3_RS14030 0.27 -0.1 3,066,793 - RALBFv3_RS14030 0.27 -0.1 3,066,793 - RALBFv3_RS14030 0.27 -0.0 3,067,065 + RALBFv3_RS14030 0.57 +0.4 3,067,065 + RALBFv3_RS14030 0.57 +0.4 3,067,065 + RALBFv3_RS14030 0.57 +0.1 3,067,188 + RALBFv3_RS14030 0.70 -0.1 3,067,188 + RALBFv3_RS14030 0.70 +0.4 3,067,188 + RALBFv3_RS14030 0.70 -0.3 3,067,188 + RALBFv3_RS14030 0.70 -0.6 3,067,189 - RALBFv3_RS14030 0.70 +0.2 3,067,189 - RALBFv3_RS14030 0.70 +0.1 3,067,189 - RALBFv3_RS14030 0.70 +0.0 3,067,189 - RALBFv3_RS14030 0.70 +0.2 3,067,189 - RALBFv3_RS14030 0.70 +1.0 3,067,293 + RALBFv3_RS14030 0.82 -0.3 3,067,294 - RALBFv3_RS14030 0.82 -0.8 3,067,477 + +1.0 3,067,498 + +1.0
Or see this region's nucleotide sequence