Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS14025

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS14015 and RALBFv3_RS14020 are separated by 140 nucleotidesRALBFv3_RS14020 and RALBFv3_RS14025 are separated by 147 nucleotidesRALBFv3_RS14025 and RALBFv3_RS14030 are separated by 17 nucleotides RALBFv3_RS14015: RALBFv3_RS14015 - voltage-gated chloride channel, at 3,063,556 to 3,065,046 _RS14015 RALBFv3_RS14020: RALBFv3_RS14020 - hypothetical protein, at 3,065,187 to 3,065,414 _RS14020 RALBFv3_RS14025: RALBFv3_RS14025 - hypothetical protein, at 3,065,562 to 3,066,530 _RS14025 RALBFv3_RS14030: RALBFv3_RS14030 - amino acid ABC transporter substrate-binding protein, at 3,066,548 to 3,067,459 _RS14030 Position (kb) 3065 3066 3067Strain fitness (log2 ratio) -2 -1 0 1at 3065.060 kb on - strandat 3065.121 kb on - strandat 3065.121 kb on - strandat 3065.121 kb on - strandat 3065.124 kb on + strandat 3065.125 kb on + strandat 3065.157 kb on + strandat 3065.157 kb on + strandat 3065.158 kb on - strandat 3065.158 kb on - strandat 3065.165 kb on + strandat 3065.166 kb on - strandat 3065.166 kb on - strandat 3065.186 kb on + strandat 3065.187 kb on - strandat 3065.187 kb on - strandat 3065.198 kb on + strandat 3065.199 kb on - strandat 3065.199 kb on - strandat 3065.199 kb on - strandat 3065.264 kb on + strand, within RALBFv3_RS14020at 3065.298 kb on + strand, within RALBFv3_RS14020at 3065.298 kb on + strand, within RALBFv3_RS14020at 3065.298 kb on + strand, within RALBFv3_RS14020at 3065.298 kb on + strand, within RALBFv3_RS14020at 3065.299 kb on - strand, within RALBFv3_RS14020at 3065.299 kb on - strand, within RALBFv3_RS14020at 3065.412 kb on + strandat 3065.412 kb on + strandat 3065.413 kb on - strandat 3065.413 kb on - strandat 3065.413 kb on - strandat 3065.522 kb on - strandat 3065.545 kb on - strandat 3065.717 kb on + strand, within RALBFv3_RS14025at 3065.961 kb on + strand, within RALBFv3_RS14025at 3065.961 kb on + strand, within RALBFv3_RS14025at 3065.962 kb on - strand, within RALBFv3_RS14025at 3065.962 kb on - strand, within RALBFv3_RS14025at 3065.962 kb on - strand, within RALBFv3_RS14025at 3065.962 kb on - strand, within RALBFv3_RS14025at 3065.962 kb on - strand, within RALBFv3_RS14025at 3065.962 kb on - strand, within RALBFv3_RS14025at 3066.240 kb on - strand, within RALBFv3_RS14025at 3066.436 kb on - strandat 3066.660 kb on + strand, within RALBFv3_RS14030at 3066.776 kb on - strand, within RALBFv3_RS14030at 3066.793 kb on - strand, within RALBFv3_RS14030at 3066.793 kb on - strand, within RALBFv3_RS14030at 3066.793 kb on - strand, within RALBFv3_RS14030at 3067.065 kb on + strand, within RALBFv3_RS14030at 3067.065 kb on + strand, within RALBFv3_RS14030at 3067.065 kb on + strand, within RALBFv3_RS14030at 3067.188 kb on + strand, within RALBFv3_RS14030at 3067.188 kb on + strand, within RALBFv3_RS14030at 3067.188 kb on + strand, within RALBFv3_RS14030at 3067.188 kb on + strand, within RALBFv3_RS14030at 3067.189 kb on - strand, within RALBFv3_RS14030at 3067.189 kb on - strand, within RALBFv3_RS14030at 3067.189 kb on - strand, within RALBFv3_RS14030at 3067.189 kb on - strand, within RALBFv3_RS14030at 3067.189 kb on - strand, within RALBFv3_RS14030at 3067.293 kb on + strand, within RALBFv3_RS14030at 3067.294 kb on - strand, within RALBFv3_RS14030at 3067.477 kb on + strandat 3067.498 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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3,065,060 - -0.1
3,065,121 - -0.2
3,065,121 - -0.4
3,065,121 - -0.6
3,065,124 + -0.2
3,065,125 + +0.6
3,065,157 + -0.7
3,065,157 + -0.3
3,065,158 - -0.3
3,065,158 - +0.3
3,065,165 + +1.0
3,065,166 - +1.0
3,065,166 - -0.3
3,065,186 + -0.4
3,065,187 - +1.0
3,065,187 - +0.5
3,065,198 + +0.1
3,065,199 - -0.5
3,065,199 - +0.4
3,065,199 - -0.1
3,065,264 + RALBFv3_RS14020 0.34 +0.4
3,065,298 + RALBFv3_RS14020 0.49 -0.9
3,065,298 + RALBFv3_RS14020 0.49 -0.3
3,065,298 + RALBFv3_RS14020 0.49 +0.2
3,065,298 + RALBFv3_RS14020 0.49 +0.1
3,065,299 - RALBFv3_RS14020 0.49 -0.4
3,065,299 - RALBFv3_RS14020 0.49 +1.0
3,065,412 + -0.6
3,065,412 + -0.0
3,065,413 - -0.3
3,065,413 - +0.1
3,065,413 - +0.5
3,065,522 - -1.3
3,065,545 - +0.8
3,065,717 + RALBFv3_RS14025 0.16 -0.7
3,065,961 + RALBFv3_RS14025 0.41 -0.7
3,065,961 + RALBFv3_RS14025 0.41 -2.2
3,065,962 - RALBFv3_RS14025 0.41 +0.4
3,065,962 - RALBFv3_RS14025 0.41 -2.2
3,065,962 - RALBFv3_RS14025 0.41 -0.6
3,065,962 - RALBFv3_RS14025 0.41 -1.8
3,065,962 - RALBFv3_RS14025 0.41 +1.0
3,065,962 - RALBFv3_RS14025 0.41 -1.1
3,066,240 - RALBFv3_RS14025 0.70 -0.3
3,066,436 - +0.5
3,066,660 + RALBFv3_RS14030 0.12 -0.0
3,066,776 - RALBFv3_RS14030 0.25 -0.1
3,066,793 - RALBFv3_RS14030 0.27 -0.1
3,066,793 - RALBFv3_RS14030 0.27 -0.1
3,066,793 - RALBFv3_RS14030 0.27 -0.0
3,067,065 + RALBFv3_RS14030 0.57 +0.4
3,067,065 + RALBFv3_RS14030 0.57 +0.4
3,067,065 + RALBFv3_RS14030 0.57 +0.1
3,067,188 + RALBFv3_RS14030 0.70 -0.1
3,067,188 + RALBFv3_RS14030 0.70 +0.4
3,067,188 + RALBFv3_RS14030 0.70 -0.3
3,067,188 + RALBFv3_RS14030 0.70 -0.6
3,067,189 - RALBFv3_RS14030 0.70 +0.2
3,067,189 - RALBFv3_RS14030 0.70 +0.1
3,067,189 - RALBFv3_RS14030 0.70 +0.0
3,067,189 - RALBFv3_RS14030 0.70 +0.2
3,067,189 - RALBFv3_RS14030 0.70 +1.0
3,067,293 + RALBFv3_RS14030 0.82 -0.3
3,067,294 - RALBFv3_RS14030 0.82 -0.8
3,067,477 + +1.0
3,067,498 + +1.0

Or see this region's nucleotide sequence