Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS12925 and RALBFv3_RS12930 are separated by 24 nucleotides RALBFv3_RS12930 and RALBFv3_RS12935 are separated by 69 nucleotides
RALBFv3_RS12925: RALBFv3_RS12925 - translocation protein TolB, at 2,840,601 to 2,841,905
_RS12925
RALBFv3_RS12930: RALBFv3_RS12930 - peptidoglycan-associated lipoprotein, at 2,841,930 to 2,842,448
_RS12930
RALBFv3_RS12935: RALBFv3_RS12935 - tol-pal system protein YbgF, at 2,842,518 to 2,843,291
_RS12935
Position (kb)
2841
2842
2843 Strain fitness (log2 ratio)
-2
-1
0
1 at 2841.114 kb on + strand, within RALBFv3_RS12925 at 2841.115 kb on - strand, within RALBFv3_RS12925 at 2841.246 kb on + strand, within RALBFv3_RS12925 at 2841.247 kb on - strand, within RALBFv3_RS12925 at 2841.280 kb on - strand, within RALBFv3_RS12925 at 2841.401 kb on + strand, within RALBFv3_RS12925 at 2841.402 kb on - strand, within RALBFv3_RS12925 at 2841.544 kb on - strand, within RALBFv3_RS12925 at 2841.684 kb on + strand, within RALBFv3_RS12925 at 2841.685 kb on - strand, within RALBFv3_RS12925 at 2841.728 kb on + strand, within RALBFv3_RS12925 at 2841.728 kb on + strand, within RALBFv3_RS12925 at 2841.735 kb on + strand, within RALBFv3_RS12925 at 2841.735 kb on + strand, within RALBFv3_RS12925 at 2841.736 kb on - strand, within RALBFv3_RS12925 at 2842.158 kb on + strand, within RALBFv3_RS12930 at 2842.158 kb on + strand, within RALBFv3_RS12930 at 2842.159 kb on - strand, within RALBFv3_RS12930 at 2842.206 kb on + strand, within RALBFv3_RS12930 at 2842.655 kb on + strand, within RALBFv3_RS12935 at 2842.837 kb on - strand, within RALBFv3_RS12935 at 2842.839 kb on + strand, within RALBFv3_RS12935 at 2842.839 kb on + strand, within RALBFv3_RS12935 at 2842.840 kb on - strand, within RALBFv3_RS12935 at 2842.935 kb on + strand, within RALBFv3_RS12935 at 2842.936 kb on - strand, within RALBFv3_RS12935 at 2843.029 kb on - strand, within RALBFv3_RS12935 at 2843.068 kb on - strand, within RALBFv3_RS12935 at 2843.082 kb on + strand, within RALBFv3_RS12935 at 2843.097 kb on + strand, within RALBFv3_RS12935 at 2843.236 kb on - strand at 2843.309 kb on + strand at 2843.309 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 2,841,114 + RALBFv3_RS12925 0.39 -1.0 2,841,115 - RALBFv3_RS12925 0.39 +1.0 2,841,246 + RALBFv3_RS12925 0.49 +1.0 2,841,247 - RALBFv3_RS12925 0.50 -0.6 2,841,280 - RALBFv3_RS12925 0.52 +1.0 2,841,401 + RALBFv3_RS12925 0.61 -1.0 2,841,402 - RALBFv3_RS12925 0.61 -1.6 2,841,544 - RALBFv3_RS12925 0.72 -0.9 2,841,684 + RALBFv3_RS12925 0.83 -1.8 2,841,685 - RALBFv3_RS12925 0.83 -1.8 2,841,728 + RALBFv3_RS12925 0.86 -1.8 2,841,728 + RALBFv3_RS12925 0.86 -0.6 2,841,735 + RALBFv3_RS12925 0.87 -0.6 2,841,735 + RALBFv3_RS12925 0.87 -2.0 2,841,736 - RALBFv3_RS12925 0.87 -1.3 2,842,158 + RALBFv3_RS12930 0.44 +1.0 2,842,158 + RALBFv3_RS12930 0.44 -1.0 2,842,159 - RALBFv3_RS12930 0.44 -0.6 2,842,206 + RALBFv3_RS12930 0.53 +1.0 2,842,655 + RALBFv3_RS12935 0.18 -1.5 2,842,837 - RALBFv3_RS12935 0.41 -1.6 2,842,839 + RALBFv3_RS12935 0.41 -1.0 2,842,839 + RALBFv3_RS12935 0.41 -0.9 2,842,840 - RALBFv3_RS12935 0.42 -1.5 2,842,935 + RALBFv3_RS12935 0.54 -0.7 2,842,936 - RALBFv3_RS12935 0.54 -0.6 2,843,029 - RALBFv3_RS12935 0.66 -0.3 2,843,068 - RALBFv3_RS12935 0.71 -0.8 2,843,082 + RALBFv3_RS12935 0.73 -0.3 2,843,097 + RALBFv3_RS12935 0.75 -0.9 2,843,236 - -0.7 2,843,309 + -0.5 2,843,309 + -0.4
Or see this region's nucleotide sequence