Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS12690

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS12685 and RALBFv3_RS12690 overlap by 11 nucleotidesRALBFv3_RS12690 and RALBFv3_RS12695 are separated by 80 nucleotides RALBFv3_RS12685: RALBFv3_RS12685 - glycosyl transferase, at 2,794,675 to 2,795,781 _RS12685 RALBFv3_RS12690: RALBFv3_RS12690 - chain-length determining protein, at 2,795,771 to 2,796,673 _RS12690 RALBFv3_RS12695: RALBFv3_RS12695 - phosphomannomutase/phosphoglucomutase, at 2,796,754 to 2,798,139 _RS12695 Position (kb) 2795 2796 2797Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 2794.894 kb on + strand, within RALBFv3_RS12685at 2794.895 kb on - strand, within RALBFv3_RS12685at 2795.023 kb on + strand, within RALBFv3_RS12685at 2795.023 kb on + strand, within RALBFv3_RS12685at 2795.023 kb on + strand, within RALBFv3_RS12685at 2795.023 kb on + strand, within RALBFv3_RS12685at 2795.024 kb on - strand, within RALBFv3_RS12685at 2795.045 kb on + strand, within RALBFv3_RS12685at 2795.045 kb on + strand, within RALBFv3_RS12685at 2795.046 kb on - strand, within RALBFv3_RS12685at 2795.145 kb on + strand, within RALBFv3_RS12685at 2795.146 kb on - strand, within RALBFv3_RS12685at 2795.209 kb on + strand, within RALBFv3_RS12685at 2795.210 kb on - strand, within RALBFv3_RS12685at 2795.335 kb on + strand, within RALBFv3_RS12685at 2795.335 kb on + strand, within RALBFv3_RS12685at 2795.336 kb on - strand, within RALBFv3_RS12685at 2795.336 kb on - strand, within RALBFv3_RS12685at 2795.336 kb on - strand, within RALBFv3_RS12685at 2795.336 kb on - strand, within RALBFv3_RS12685at 2795.336 kb on - strand, within RALBFv3_RS12685at 2795.337 kb on + strand, within RALBFv3_RS12685at 2795.337 kb on + strand, within RALBFv3_RS12685at 2795.337 kb on + strand, within RALBFv3_RS12685at 2795.337 kb on + strand, within RALBFv3_RS12685at 2795.338 kb on - strand, within RALBFv3_RS12685at 2795.338 kb on - strand, within RALBFv3_RS12685at 2795.338 kb on - strand, within RALBFv3_RS12685at 2795.338 kb on - strand, within RALBFv3_RS12685at 2795.527 kb on + strand, within RALBFv3_RS12685at 2795.605 kb on + strand, within RALBFv3_RS12685at 2795.704 kb on + strandat 2795.704 kb on + strandat 2795.704 kb on + strandat 2795.705 kb on - strandat 2796.223 kb on + strand, within RALBFv3_RS12690at 2796.223 kb on + strand, within RALBFv3_RS12690at 2796.223 kb on + strand, within RALBFv3_RS12690at 2796.223 kb on + strand, within RALBFv3_RS12690at 2796.223 kb on + strand, within RALBFv3_RS12690at 2796.264 kb on + strand, within RALBFv3_RS12690at 2796.681 kb on + strandat 2796.681 kb on + strandat 2797.195 kb on + strand, within RALBFv3_RS12695

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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2,794,894 + RALBFv3_RS12685 0.20 -4.0
2,794,895 - RALBFv3_RS12685 0.20 -3.1
2,795,023 + RALBFv3_RS12685 0.31 -4.7
2,795,023 + RALBFv3_RS12685 0.31 -4.8
2,795,023 + RALBFv3_RS12685 0.31 -2.2
2,795,023 + RALBFv3_RS12685 0.31 -1.0
2,795,024 - RALBFv3_RS12685 0.32 -2.3
2,795,045 + RALBFv3_RS12685 0.33 -3.2
2,795,045 + RALBFv3_RS12685 0.33 -2.0
2,795,046 - RALBFv3_RS12685 0.34 -2.4
2,795,145 + RALBFv3_RS12685 0.42 -4.1
2,795,146 - RALBFv3_RS12685 0.43 -4.3
2,795,209 + RALBFv3_RS12685 0.48 +1.0
2,795,210 - RALBFv3_RS12685 0.48 -2.7
2,795,335 + RALBFv3_RS12685 0.60 -1.8
2,795,335 + RALBFv3_RS12685 0.60 -1.8
2,795,336 - RALBFv3_RS12685 0.60 -1.7
2,795,336 - RALBFv3_RS12685 0.60 -3.8
2,795,336 - RALBFv3_RS12685 0.60 -4.1
2,795,336 - RALBFv3_RS12685 0.60 -2.8
2,795,336 - RALBFv3_RS12685 0.60 -3.5
2,795,337 + RALBFv3_RS12685 0.60 -3.7
2,795,337 + RALBFv3_RS12685 0.60 -4.4
2,795,337 + RALBFv3_RS12685 0.60 -3.8
2,795,337 + RALBFv3_RS12685 0.60 -3.1
2,795,338 - RALBFv3_RS12685 0.60 -1.3
2,795,338 - RALBFv3_RS12685 0.60 -2.1
2,795,338 - RALBFv3_RS12685 0.60 -2.8
2,795,338 - RALBFv3_RS12685 0.60 -3.1
2,795,527 + RALBFv3_RS12685 0.77 -5.4
2,795,605 + RALBFv3_RS12685 0.84 -2.0
2,795,704 + -1.8
2,795,704 + -3.0
2,795,704 + -3.2
2,795,705 - -2.0
2,796,223 + RALBFv3_RS12690 0.50 -1.2
2,796,223 + RALBFv3_RS12690 0.50 -0.8
2,796,223 + RALBFv3_RS12690 0.50 -1.2
2,796,223 + RALBFv3_RS12690 0.50 -1.0
2,796,223 + RALBFv3_RS12690 0.50 -0.9
2,796,264 + RALBFv3_RS12690 0.55 -0.7
2,796,681 + +1.0
2,796,681 + -0.3
2,797,195 + RALBFv3_RS12695 0.32 -0.6

Or see this region's nucleotide sequence