Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS12675 and RALBFv3_RS12680 are separated by 79 nucleotides RALBFv3_RS12680 and RALBFv3_RS12685 are separated by 22 nucleotides RALBFv3_RS12685 and RALBFv3_RS12690 overlap by 11 nucleotides RALBFv3_RS12690 and RALBFv3_RS12695 are separated by 80 nucleotides
RALBFv3_RS12675: RALBFv3_RS12675 - EpsG family protein, at 2,792,783 to 2,793,745
_RS12675
RALBFv3_RS12680: RALBFv3_RS12680 - glycosyltransferase family 2 protein, at 2,793,825 to 2,794,652
_RS12680
RALBFv3_RS12685: RALBFv3_RS12685 - glycosyl transferase, at 2,794,675 to 2,795,781
_RS12685
RALBFv3_RS12690: RALBFv3_RS12690 - chain-length determining protein, at 2,795,771 to 2,796,673
_RS12690
RALBFv3_RS12695: RALBFv3_RS12695 - phosphomannomutase/phosphoglucomutase, at 2,796,754 to 2,798,139
_RS12695
Position (kb)
2794
2795
2796 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2793.687 kb on - strand at 2793.687 kb on - strand at 2793.830 kb on + strand at 2793.831 kb on - strand at 2793.833 kb on + strand at 2793.834 kb on - strand at 2793.837 kb on - strand at 2793.856 kb on - strand at 2793.958 kb on - strand, within RALBFv3_RS12680 at 2793.958 kb on - strand, within RALBFv3_RS12680 at 2793.958 kb on - strand, within RALBFv3_RS12680 at 2794.029 kb on - strand, within RALBFv3_RS12680 at 2794.198 kb on - strand, within RALBFv3_RS12680 at 2794.209 kb on - strand, within RALBFv3_RS12680 at 2794.209 kb on - strand, within RALBFv3_RS12680 at 2794.209 kb on - strand, within RALBFv3_RS12680 at 2794.309 kb on - strand, within RALBFv3_RS12680 at 2794.309 kb on - strand, within RALBFv3_RS12680 at 2794.309 kb on - strand, within RALBFv3_RS12680 at 2794.411 kb on - strand, within RALBFv3_RS12680 at 2794.411 kb on - strand, within RALBFv3_RS12680 at 2794.411 kb on - strand, within RALBFv3_RS12680 at 2794.427 kb on + strand, within RALBFv3_RS12680 at 2794.428 kb on - strand, within RALBFv3_RS12680 at 2794.428 kb on - strand, within RALBFv3_RS12680 at 2794.428 kb on - strand, within RALBFv3_RS12680 at 2794.428 kb on - strand, within RALBFv3_RS12680 at 2794.607 kb on + strand at 2794.628 kb on - strand at 2794.628 kb on - strand at 2794.675 kb on - strand at 2794.680 kb on + strand at 2794.680 kb on + strand at 2794.680 kb on + strand at 2794.681 kb on - strand at 2794.759 kb on - strand at 2794.894 kb on + strand, within RALBFv3_RS12685 at 2794.895 kb on - strand, within RALBFv3_RS12685 at 2795.023 kb on + strand, within RALBFv3_RS12685 at 2795.023 kb on + strand, within RALBFv3_RS12685 at 2795.023 kb on + strand, within RALBFv3_RS12685 at 2795.023 kb on + strand, within RALBFv3_RS12685 at 2795.024 kb on - strand, within RALBFv3_RS12685 at 2795.045 kb on + strand, within RALBFv3_RS12685 at 2795.045 kb on + strand, within RALBFv3_RS12685 at 2795.046 kb on - strand, within RALBFv3_RS12685 at 2795.145 kb on + strand, within RALBFv3_RS12685 at 2795.146 kb on - strand, within RALBFv3_RS12685 at 2795.209 kb on + strand, within RALBFv3_RS12685 at 2795.210 kb on - strand, within RALBFv3_RS12685 at 2795.335 kb on + strand, within RALBFv3_RS12685 at 2795.335 kb on + strand, within RALBFv3_RS12685 at 2795.336 kb on - strand, within RALBFv3_RS12685 at 2795.336 kb on - strand, within RALBFv3_RS12685 at 2795.336 kb on - strand, within RALBFv3_RS12685 at 2795.336 kb on - strand, within RALBFv3_RS12685 at 2795.336 kb on - strand, within RALBFv3_RS12685 at 2795.337 kb on + strand, within RALBFv3_RS12685 at 2795.337 kb on + strand, within RALBFv3_RS12685 at 2795.337 kb on + strand, within RALBFv3_RS12685 at 2795.337 kb on + strand, within RALBFv3_RS12685 at 2795.338 kb on - strand, within RALBFv3_RS12685 at 2795.338 kb on - strand, within RALBFv3_RS12685 at 2795.338 kb on - strand, within RALBFv3_RS12685 at 2795.338 kb on - strand, within RALBFv3_RS12685 at 2795.527 kb on + strand, within RALBFv3_RS12685 at 2795.605 kb on + strand, within RALBFv3_RS12685 at 2795.704 kb on + strand at 2795.704 kb on + strand at 2795.704 kb on + strand at 2795.705 kb on - strand at 2796.223 kb on + strand, within RALBFv3_RS12690 at 2796.223 kb on + strand, within RALBFv3_RS12690 at 2796.223 kb on + strand, within RALBFv3_RS12690 at 2796.223 kb on + strand, within RALBFv3_RS12690 at 2796.223 kb on + strand, within RALBFv3_RS12690 at 2796.264 kb on + strand, within RALBFv3_RS12690 at 2796.681 kb on + strand at 2796.681 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 2,793,687 - -2.7 2,793,687 - -1.0 2,793,830 + -1.8 2,793,831 - -3.0 2,793,833 + -2.3 2,793,834 - -3.3 2,793,837 - -3.4 2,793,856 - -4.1 2,793,958 - RALBFv3_RS12680 0.16 -2.2 2,793,958 - RALBFv3_RS12680 0.16 -3.1 2,793,958 - RALBFv3_RS12680 0.16 -2.3 2,794,029 - RALBFv3_RS12680 0.25 -3.0 2,794,198 - RALBFv3_RS12680 0.45 -3.8 2,794,209 - RALBFv3_RS12680 0.46 -0.6 2,794,209 - RALBFv3_RS12680 0.46 -3.6 2,794,209 - RALBFv3_RS12680 0.46 -2.6 2,794,309 - RALBFv3_RS12680 0.58 -4.0 2,794,309 - RALBFv3_RS12680 0.58 -0.6 2,794,309 - RALBFv3_RS12680 0.58 +0.0 2,794,411 - RALBFv3_RS12680 0.71 -4.3 2,794,411 - RALBFv3_RS12680 0.71 -1.8 2,794,411 - RALBFv3_RS12680 0.71 -0.6 2,794,427 + RALBFv3_RS12680 0.73 -1.0 2,794,428 - RALBFv3_RS12680 0.73 -2.7 2,794,428 - RALBFv3_RS12680 0.73 -0.6 2,794,428 - RALBFv3_RS12680 0.73 -0.3 2,794,428 - RALBFv3_RS12680 0.73 -1.0 2,794,607 + -3.1 2,794,628 - -1.0 2,794,628 - -2.7 2,794,675 - -1.6 2,794,680 + -3.9 2,794,680 + -3.4 2,794,680 + -2.8 2,794,681 - -2.2 2,794,759 - -2.2 2,794,894 + RALBFv3_RS12685 0.20 -4.0 2,794,895 - RALBFv3_RS12685 0.20 -3.1 2,795,023 + RALBFv3_RS12685 0.31 -4.7 2,795,023 + RALBFv3_RS12685 0.31 -4.8 2,795,023 + RALBFv3_RS12685 0.31 -2.2 2,795,023 + RALBFv3_RS12685 0.31 -1.0 2,795,024 - RALBFv3_RS12685 0.32 -2.3 2,795,045 + RALBFv3_RS12685 0.33 -3.2 2,795,045 + RALBFv3_RS12685 0.33 -2.0 2,795,046 - RALBFv3_RS12685 0.34 -2.4 2,795,145 + RALBFv3_RS12685 0.42 -4.1 2,795,146 - RALBFv3_RS12685 0.43 -4.3 2,795,209 + RALBFv3_RS12685 0.48 +1.0 2,795,210 - RALBFv3_RS12685 0.48 -2.7 2,795,335 + RALBFv3_RS12685 0.60 -1.8 2,795,335 + RALBFv3_RS12685 0.60 -1.8 2,795,336 - RALBFv3_RS12685 0.60 -1.7 2,795,336 - RALBFv3_RS12685 0.60 -3.8 2,795,336 - RALBFv3_RS12685 0.60 -4.1 2,795,336 - RALBFv3_RS12685 0.60 -2.8 2,795,336 - RALBFv3_RS12685 0.60 -3.5 2,795,337 + RALBFv3_RS12685 0.60 -3.7 2,795,337 + RALBFv3_RS12685 0.60 -4.4 2,795,337 + RALBFv3_RS12685 0.60 -3.8 2,795,337 + RALBFv3_RS12685 0.60 -3.1 2,795,338 - RALBFv3_RS12685 0.60 -1.3 2,795,338 - RALBFv3_RS12685 0.60 -2.1 2,795,338 - RALBFv3_RS12685 0.60 -2.8 2,795,338 - RALBFv3_RS12685 0.60 -3.1 2,795,527 + RALBFv3_RS12685 0.77 -5.4 2,795,605 + RALBFv3_RS12685 0.84 -2.0 2,795,704 + -1.8 2,795,704 + -3.0 2,795,704 + -3.2 2,795,705 - -2.0 2,796,223 + RALBFv3_RS12690 0.50 -1.2 2,796,223 + RALBFv3_RS12690 0.50 -0.8 2,796,223 + RALBFv3_RS12690 0.50 -1.2 2,796,223 + RALBFv3_RS12690 0.50 -1.0 2,796,223 + RALBFv3_RS12690 0.50 -0.9 2,796,264 + RALBFv3_RS12690 0.55 -0.7 2,796,681 + +1.0 2,796,681 + -0.3
Or see this region's nucleotide sequence