Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS12680

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS12675 and RALBFv3_RS12680 are separated by 79 nucleotidesRALBFv3_RS12680 and RALBFv3_RS12685 are separated by 22 nucleotides RALBFv3_RS12675: RALBFv3_RS12675 - EpsG family protein, at 2,792,783 to 2,793,745 _RS12675 RALBFv3_RS12680: RALBFv3_RS12680 - glycosyltransferase family 2 protein, at 2,793,825 to 2,794,652 _RS12680 RALBFv3_RS12685: RALBFv3_RS12685 - glycosyl transferase, at 2,794,675 to 2,795,781 _RS12685 Position (kb) 2793 2794 2795Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 2792.829 kb on + strandat 2792.912 kb on + strand, within RALBFv3_RS12675at 2792.912 kb on + strand, within RALBFv3_RS12675at 2792.913 kb on - strand, within RALBFv3_RS12675at 2792.930 kb on + strand, within RALBFv3_RS12675at 2792.989 kb on + strand, within RALBFv3_RS12675at 2792.989 kb on + strand, within RALBFv3_RS12675at 2793.019 kb on + strand, within RALBFv3_RS12675at 2793.019 kb on + strand, within RALBFv3_RS12675at 2793.019 kb on + strand, within RALBFv3_RS12675at 2793.037 kb on - strand, within RALBFv3_RS12675at 2793.128 kb on + strand, within RALBFv3_RS12675at 2793.267 kb on - strand, within RALBFv3_RS12675at 2793.359 kb on + strand, within RALBFv3_RS12675at 2793.359 kb on + strand, within RALBFv3_RS12675at 2793.359 kb on + strand, within RALBFv3_RS12675at 2793.359 kb on + strand, within RALBFv3_RS12675at 2793.360 kb on - strand, within RALBFv3_RS12675at 2793.384 kb on - strand, within RALBFv3_RS12675at 2793.395 kb on + strand, within RALBFv3_RS12675at 2793.395 kb on + strand, within RALBFv3_RS12675at 2793.395 kb on + strand, within RALBFv3_RS12675at 2793.542 kb on + strand, within RALBFv3_RS12675at 2793.542 kb on + strand, within RALBFv3_RS12675at 2793.599 kb on + strand, within RALBFv3_RS12675at 2793.599 kb on + strand, within RALBFv3_RS12675at 2793.600 kb on - strand, within RALBFv3_RS12675at 2793.687 kb on - strandat 2793.687 kb on - strandat 2793.830 kb on + strandat 2793.831 kb on - strandat 2793.833 kb on + strandat 2793.834 kb on - strandat 2793.837 kb on - strandat 2793.856 kb on - strandat 2793.958 kb on - strand, within RALBFv3_RS12680at 2793.958 kb on - strand, within RALBFv3_RS12680at 2793.958 kb on - strand, within RALBFv3_RS12680at 2794.029 kb on - strand, within RALBFv3_RS12680at 2794.198 kb on - strand, within RALBFv3_RS12680at 2794.209 kb on - strand, within RALBFv3_RS12680at 2794.209 kb on - strand, within RALBFv3_RS12680at 2794.209 kb on - strand, within RALBFv3_RS12680at 2794.309 kb on - strand, within RALBFv3_RS12680at 2794.309 kb on - strand, within RALBFv3_RS12680at 2794.309 kb on - strand, within RALBFv3_RS12680at 2794.411 kb on - strand, within RALBFv3_RS12680at 2794.411 kb on - strand, within RALBFv3_RS12680at 2794.411 kb on - strand, within RALBFv3_RS12680at 2794.427 kb on + strand, within RALBFv3_RS12680at 2794.428 kb on - strand, within RALBFv3_RS12680at 2794.428 kb on - strand, within RALBFv3_RS12680at 2794.428 kb on - strand, within RALBFv3_RS12680at 2794.428 kb on - strand, within RALBFv3_RS12680at 2794.607 kb on + strandat 2794.628 kb on - strandat 2794.628 kb on - strandat 2794.675 kb on - strandat 2794.680 kb on + strandat 2794.680 kb on + strandat 2794.680 kb on + strandat 2794.681 kb on - strandat 2794.759 kb on - strandat 2794.894 kb on + strand, within RALBFv3_RS12685at 2794.895 kb on - strand, within RALBFv3_RS12685at 2795.023 kb on + strand, within RALBFv3_RS12685at 2795.023 kb on + strand, within RALBFv3_RS12685at 2795.023 kb on + strand, within RALBFv3_RS12685at 2795.023 kb on + strand, within RALBFv3_RS12685at 2795.024 kb on - strand, within RALBFv3_RS12685at 2795.045 kb on + strand, within RALBFv3_RS12685at 2795.045 kb on + strand, within RALBFv3_RS12685at 2795.046 kb on - strand, within RALBFv3_RS12685at 2795.145 kb on + strand, within RALBFv3_RS12685at 2795.146 kb on - strand, within RALBFv3_RS12685at 2795.209 kb on + strand, within RALBFv3_RS12685at 2795.210 kb on - strand, within RALBFv3_RS12685at 2795.335 kb on + strand, within RALBFv3_RS12685at 2795.335 kb on + strand, within RALBFv3_RS12685at 2795.336 kb on - strand, within RALBFv3_RS12685at 2795.336 kb on - strand, within RALBFv3_RS12685at 2795.336 kb on - strand, within RALBFv3_RS12685at 2795.336 kb on - strand, within RALBFv3_RS12685at 2795.336 kb on - strand, within RALBFv3_RS12685at 2795.337 kb on + strand, within RALBFv3_RS12685at 2795.337 kb on + strand, within RALBFv3_RS12685at 2795.337 kb on + strand, within RALBFv3_RS12685at 2795.337 kb on + strand, within RALBFv3_RS12685at 2795.338 kb on - strand, within RALBFv3_RS12685at 2795.338 kb on - strand, within RALBFv3_RS12685at 2795.338 kb on - strand, within RALBFv3_RS12685at 2795.338 kb on - strand, within RALBFv3_RS12685at 2795.527 kb on + strand, within RALBFv3_RS12685at 2795.605 kb on + strand, within RALBFv3_RS12685

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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2,792,829 + -0.1
2,792,912 + RALBFv3_RS12675 0.13 -0.6
2,792,912 + RALBFv3_RS12675 0.13 -1.6
2,792,913 - RALBFv3_RS12675 0.13 -1.3
2,792,930 + RALBFv3_RS12675 0.15 -1.6
2,792,989 + RALBFv3_RS12675 0.21 -1.3
2,792,989 + RALBFv3_RS12675 0.21 +0.4
2,793,019 + RALBFv3_RS12675 0.25 -1.8
2,793,019 + RALBFv3_RS12675 0.25 +0.3
2,793,019 + RALBFv3_RS12675 0.25 -1.7
2,793,037 - RALBFv3_RS12675 0.26 -1.8
2,793,128 + RALBFv3_RS12675 0.36 -1.4
2,793,267 - RALBFv3_RS12675 0.50 -3.2
2,793,359 + RALBFv3_RS12675 0.60 -1.6
2,793,359 + RALBFv3_RS12675 0.60 -3.6
2,793,359 + RALBFv3_RS12675 0.60 -2.0
2,793,359 + RALBFv3_RS12675 0.60 -1.2
2,793,360 - RALBFv3_RS12675 0.60 -1.3
2,793,384 - RALBFv3_RS12675 0.62 -2.2
2,793,395 + RALBFv3_RS12675 0.64 -2.0
2,793,395 + RALBFv3_RS12675 0.64 -1.6
2,793,395 + RALBFv3_RS12675 0.64 -1.3
2,793,542 + RALBFv3_RS12675 0.79 -2.5
2,793,542 + RALBFv3_RS12675 0.79 -2.3
2,793,599 + RALBFv3_RS12675 0.85 -2.3
2,793,599 + RALBFv3_RS12675 0.85 -2.6
2,793,600 - RALBFv3_RS12675 0.85 -1.0
2,793,687 - -2.7
2,793,687 - -1.0
2,793,830 + -1.8
2,793,831 - -3.0
2,793,833 + -2.3
2,793,834 - -3.3
2,793,837 - -3.4
2,793,856 - -4.1
2,793,958 - RALBFv3_RS12680 0.16 -2.2
2,793,958 - RALBFv3_RS12680 0.16 -3.1
2,793,958 - RALBFv3_RS12680 0.16 -2.3
2,794,029 - RALBFv3_RS12680 0.25 -3.0
2,794,198 - RALBFv3_RS12680 0.45 -3.8
2,794,209 - RALBFv3_RS12680 0.46 -0.6
2,794,209 - RALBFv3_RS12680 0.46 -3.6
2,794,209 - RALBFv3_RS12680 0.46 -2.6
2,794,309 - RALBFv3_RS12680 0.58 -4.0
2,794,309 - RALBFv3_RS12680 0.58 -0.6
2,794,309 - RALBFv3_RS12680 0.58 +0.0
2,794,411 - RALBFv3_RS12680 0.71 -4.3
2,794,411 - RALBFv3_RS12680 0.71 -1.8
2,794,411 - RALBFv3_RS12680 0.71 -0.6
2,794,427 + RALBFv3_RS12680 0.73 -1.0
2,794,428 - RALBFv3_RS12680 0.73 -2.7
2,794,428 - RALBFv3_RS12680 0.73 -0.6
2,794,428 - RALBFv3_RS12680 0.73 -0.3
2,794,428 - RALBFv3_RS12680 0.73 -1.0
2,794,607 + -3.1
2,794,628 - -1.0
2,794,628 - -2.7
2,794,675 - -1.6
2,794,680 + -3.9
2,794,680 + -3.4
2,794,680 + -2.8
2,794,681 - -2.2
2,794,759 - -2.2
2,794,894 + RALBFv3_RS12685 0.20 -4.0
2,794,895 - RALBFv3_RS12685 0.20 -3.1
2,795,023 + RALBFv3_RS12685 0.31 -4.7
2,795,023 + RALBFv3_RS12685 0.31 -4.8
2,795,023 + RALBFv3_RS12685 0.31 -2.2
2,795,023 + RALBFv3_RS12685 0.31 -1.0
2,795,024 - RALBFv3_RS12685 0.32 -2.3
2,795,045 + RALBFv3_RS12685 0.33 -3.2
2,795,045 + RALBFv3_RS12685 0.33 -2.0
2,795,046 - RALBFv3_RS12685 0.34 -2.4
2,795,145 + RALBFv3_RS12685 0.42 -4.1
2,795,146 - RALBFv3_RS12685 0.43 -4.3
2,795,209 + RALBFv3_RS12685 0.48 +1.0
2,795,210 - RALBFv3_RS12685 0.48 -2.7
2,795,335 + RALBFv3_RS12685 0.60 -1.8
2,795,335 + RALBFv3_RS12685 0.60 -1.8
2,795,336 - RALBFv3_RS12685 0.60 -1.7
2,795,336 - RALBFv3_RS12685 0.60 -3.8
2,795,336 - RALBFv3_RS12685 0.60 -4.1
2,795,336 - RALBFv3_RS12685 0.60 -2.8
2,795,336 - RALBFv3_RS12685 0.60 -3.5
2,795,337 + RALBFv3_RS12685 0.60 -3.7
2,795,337 + RALBFv3_RS12685 0.60 -4.4
2,795,337 + RALBFv3_RS12685 0.60 -3.8
2,795,337 + RALBFv3_RS12685 0.60 -3.1
2,795,338 - RALBFv3_RS12685 0.60 -1.3
2,795,338 - RALBFv3_RS12685 0.60 -2.1
2,795,338 - RALBFv3_RS12685 0.60 -2.8
2,795,338 - RALBFv3_RS12685 0.60 -3.1
2,795,527 + RALBFv3_RS12685 0.77 -5.4
2,795,605 + RALBFv3_RS12685 0.84 -2.0

Or see this region's nucleotide sequence