Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS12665 and RALBFv3_RS12670 are separated by 38 nucleotides RALBFv3_RS12670 and RALBFv3_RS12675 are separated by 84 nucleotides
RALBFv3_RS12665: RALBFv3_RS12665 - Oligosaccharide translocase (flippase), at 2,790,462 to 2,791,730
_RS12665
RALBFv3_RS12670: RALBFv3_RS12670 - glycosyltransferase family 2 protein, at 2,791,769 to 2,792,698
_RS12670
RALBFv3_RS12675: RALBFv3_RS12675 - EpsG family protein, at 2,792,783 to 2,793,745
_RS12675
Position (kb)
2791
2792
2793 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2791.688 kb on + strand at 2792.019 kb on - strand, within RALBFv3_RS12670 at 2792.121 kb on - strand, within RALBFv3_RS12670 at 2792.121 kb on - strand, within RALBFv3_RS12670 at 2792.121 kb on - strand, within RALBFv3_RS12670 at 2792.295 kb on - strand, within RALBFv3_RS12670 at 2792.295 kb on - strand, within RALBFv3_RS12670 at 2792.322 kb on - strand, within RALBFv3_RS12670 at 2792.377 kb on - strand, within RALBFv3_RS12670 at 2792.377 kb on - strand, within RALBFv3_RS12670 at 2792.530 kb on - strand, within RALBFv3_RS12670 at 2792.534 kb on - strand, within RALBFv3_RS12670 at 2792.577 kb on - strand, within RALBFv3_RS12670 at 2792.769 kb on + strand at 2792.769 kb on + strand at 2792.769 kb on + strand at 2792.769 kb on + strand at 2792.770 kb on - strand at 2792.782 kb on + strand at 2792.829 kb on + strand at 2792.912 kb on + strand, within RALBFv3_RS12675 at 2792.912 kb on + strand, within RALBFv3_RS12675 at 2792.913 kb on - strand, within RALBFv3_RS12675 at 2792.930 kb on + strand, within RALBFv3_RS12675 at 2792.989 kb on + strand, within RALBFv3_RS12675 at 2792.989 kb on + strand, within RALBFv3_RS12675 at 2793.019 kb on + strand, within RALBFv3_RS12675 at 2793.019 kb on + strand, within RALBFv3_RS12675 at 2793.019 kb on + strand, within RALBFv3_RS12675 at 2793.037 kb on - strand, within RALBFv3_RS12675 at 2793.128 kb on + strand, within RALBFv3_RS12675 at 2793.267 kb on - strand, within RALBFv3_RS12675 at 2793.359 kb on + strand, within RALBFv3_RS12675 at 2793.359 kb on + strand, within RALBFv3_RS12675 at 2793.359 kb on + strand, within RALBFv3_RS12675 at 2793.359 kb on + strand, within RALBFv3_RS12675 at 2793.360 kb on - strand, within RALBFv3_RS12675 at 2793.384 kb on - strand, within RALBFv3_RS12675 at 2793.395 kb on + strand, within RALBFv3_RS12675 at 2793.395 kb on + strand, within RALBFv3_RS12675 at 2793.395 kb on + strand, within RALBFv3_RS12675 at 2793.542 kb on + strand, within RALBFv3_RS12675 at 2793.542 kb on + strand, within RALBFv3_RS12675 at 2793.599 kb on + strand, within RALBFv3_RS12675 at 2793.599 kb on + strand, within RALBFv3_RS12675 at 2793.600 kb on - strand, within RALBFv3_RS12675 at 2793.687 kb on - strand at 2793.687 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 2,791,688 + +0.7 2,792,019 - RALBFv3_RS12670 0.27 -3.1 2,792,121 - RALBFv3_RS12670 0.38 -3.7 2,792,121 - RALBFv3_RS12670 0.38 -2.2 2,792,121 - RALBFv3_RS12670 0.38 -4.6 2,792,295 - RALBFv3_RS12670 0.57 -3.5 2,792,295 - RALBFv3_RS12670 0.57 -4.3 2,792,322 - RALBFv3_RS12670 0.59 -1.2 2,792,377 - RALBFv3_RS12670 0.65 -2.2 2,792,377 - RALBFv3_RS12670 0.65 -2.1 2,792,530 - RALBFv3_RS12670 0.82 -3.4 2,792,534 - RALBFv3_RS12670 0.82 -3.6 2,792,577 - RALBFv3_RS12670 0.87 -1.6 2,792,769 + +0.7 2,792,769 + +0.9 2,792,769 + +0.3 2,792,769 + +1.3 2,792,770 - -2.5 2,792,782 + +0.3 2,792,829 + -0.1 2,792,912 + RALBFv3_RS12675 0.13 -0.6 2,792,912 + RALBFv3_RS12675 0.13 -1.6 2,792,913 - RALBFv3_RS12675 0.13 -1.3 2,792,930 + RALBFv3_RS12675 0.15 -1.6 2,792,989 + RALBFv3_RS12675 0.21 -1.3 2,792,989 + RALBFv3_RS12675 0.21 +0.4 2,793,019 + RALBFv3_RS12675 0.25 -1.8 2,793,019 + RALBFv3_RS12675 0.25 +0.3 2,793,019 + RALBFv3_RS12675 0.25 -1.7 2,793,037 - RALBFv3_RS12675 0.26 -1.8 2,793,128 + RALBFv3_RS12675 0.36 -1.4 2,793,267 - RALBFv3_RS12675 0.50 -3.2 2,793,359 + RALBFv3_RS12675 0.60 -1.6 2,793,359 + RALBFv3_RS12675 0.60 -3.6 2,793,359 + RALBFv3_RS12675 0.60 -2.0 2,793,359 + RALBFv3_RS12675 0.60 -1.2 2,793,360 - RALBFv3_RS12675 0.60 -1.3 2,793,384 - RALBFv3_RS12675 0.62 -2.2 2,793,395 + RALBFv3_RS12675 0.64 -2.0 2,793,395 + RALBFv3_RS12675 0.64 -1.6 2,793,395 + RALBFv3_RS12675 0.64 -1.3 2,793,542 + RALBFv3_RS12675 0.79 -2.5 2,793,542 + RALBFv3_RS12675 0.79 -2.3 2,793,599 + RALBFv3_RS12675 0.85 -2.3 2,793,599 + RALBFv3_RS12675 0.85 -2.6 2,793,600 - RALBFv3_RS12675 0.85 -1.0 2,793,687 - -2.7 2,793,687 - -1.0
Or see this region's nucleotide sequence