Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS12670

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS12665 and RALBFv3_RS12670 are separated by 38 nucleotidesRALBFv3_RS12670 and RALBFv3_RS12675 are separated by 84 nucleotides RALBFv3_RS12665: RALBFv3_RS12665 - Oligosaccharide translocase (flippase), at 2,790,462 to 2,791,730 _RS12665 RALBFv3_RS12670: RALBFv3_RS12670 - glycosyltransferase family 2 protein, at 2,791,769 to 2,792,698 _RS12670 RALBFv3_RS12675: RALBFv3_RS12675 - EpsG family protein, at 2,792,783 to 2,793,745 _RS12675 Position (kb) 2791 2792 2793Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 2791.688 kb on + strandat 2792.019 kb on - strand, within RALBFv3_RS12670at 2792.121 kb on - strand, within RALBFv3_RS12670at 2792.121 kb on - strand, within RALBFv3_RS12670at 2792.121 kb on - strand, within RALBFv3_RS12670at 2792.295 kb on - strand, within RALBFv3_RS12670at 2792.295 kb on - strand, within RALBFv3_RS12670at 2792.322 kb on - strand, within RALBFv3_RS12670at 2792.377 kb on - strand, within RALBFv3_RS12670at 2792.377 kb on - strand, within RALBFv3_RS12670at 2792.530 kb on - strand, within RALBFv3_RS12670at 2792.534 kb on - strand, within RALBFv3_RS12670at 2792.577 kb on - strand, within RALBFv3_RS12670at 2792.769 kb on + strandat 2792.769 kb on + strandat 2792.769 kb on + strandat 2792.769 kb on + strandat 2792.770 kb on - strandat 2792.782 kb on + strandat 2792.829 kb on + strandat 2792.912 kb on + strand, within RALBFv3_RS12675at 2792.912 kb on + strand, within RALBFv3_RS12675at 2792.913 kb on - strand, within RALBFv3_RS12675at 2792.930 kb on + strand, within RALBFv3_RS12675at 2792.989 kb on + strand, within RALBFv3_RS12675at 2792.989 kb on + strand, within RALBFv3_RS12675at 2793.019 kb on + strand, within RALBFv3_RS12675at 2793.019 kb on + strand, within RALBFv3_RS12675at 2793.019 kb on + strand, within RALBFv3_RS12675at 2793.037 kb on - strand, within RALBFv3_RS12675at 2793.128 kb on + strand, within RALBFv3_RS12675at 2793.267 kb on - strand, within RALBFv3_RS12675at 2793.359 kb on + strand, within RALBFv3_RS12675at 2793.359 kb on + strand, within RALBFv3_RS12675at 2793.359 kb on + strand, within RALBFv3_RS12675at 2793.359 kb on + strand, within RALBFv3_RS12675at 2793.360 kb on - strand, within RALBFv3_RS12675at 2793.384 kb on - strand, within RALBFv3_RS12675at 2793.395 kb on + strand, within RALBFv3_RS12675at 2793.395 kb on + strand, within RALBFv3_RS12675at 2793.395 kb on + strand, within RALBFv3_RS12675at 2793.542 kb on + strand, within RALBFv3_RS12675at 2793.542 kb on + strand, within RALBFv3_RS12675at 2793.599 kb on + strand, within RALBFv3_RS12675at 2793.599 kb on + strand, within RALBFv3_RS12675at 2793.600 kb on - strand, within RALBFv3_RS12675at 2793.687 kb on - strandat 2793.687 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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2,791,688 + +0.7
2,792,019 - RALBFv3_RS12670 0.27 -3.1
2,792,121 - RALBFv3_RS12670 0.38 -3.7
2,792,121 - RALBFv3_RS12670 0.38 -2.2
2,792,121 - RALBFv3_RS12670 0.38 -4.6
2,792,295 - RALBFv3_RS12670 0.57 -3.5
2,792,295 - RALBFv3_RS12670 0.57 -4.3
2,792,322 - RALBFv3_RS12670 0.59 -1.2
2,792,377 - RALBFv3_RS12670 0.65 -2.2
2,792,377 - RALBFv3_RS12670 0.65 -2.1
2,792,530 - RALBFv3_RS12670 0.82 -3.4
2,792,534 - RALBFv3_RS12670 0.82 -3.6
2,792,577 - RALBFv3_RS12670 0.87 -1.6
2,792,769 + +0.7
2,792,769 + +0.9
2,792,769 + +0.3
2,792,769 + +1.3
2,792,770 - -2.5
2,792,782 + +0.3
2,792,829 + -0.1
2,792,912 + RALBFv3_RS12675 0.13 -0.6
2,792,912 + RALBFv3_RS12675 0.13 -1.6
2,792,913 - RALBFv3_RS12675 0.13 -1.3
2,792,930 + RALBFv3_RS12675 0.15 -1.6
2,792,989 + RALBFv3_RS12675 0.21 -1.3
2,792,989 + RALBFv3_RS12675 0.21 +0.4
2,793,019 + RALBFv3_RS12675 0.25 -1.8
2,793,019 + RALBFv3_RS12675 0.25 +0.3
2,793,019 + RALBFv3_RS12675 0.25 -1.7
2,793,037 - RALBFv3_RS12675 0.26 -1.8
2,793,128 + RALBFv3_RS12675 0.36 -1.4
2,793,267 - RALBFv3_RS12675 0.50 -3.2
2,793,359 + RALBFv3_RS12675 0.60 -1.6
2,793,359 + RALBFv3_RS12675 0.60 -3.6
2,793,359 + RALBFv3_RS12675 0.60 -2.0
2,793,359 + RALBFv3_RS12675 0.60 -1.2
2,793,360 - RALBFv3_RS12675 0.60 -1.3
2,793,384 - RALBFv3_RS12675 0.62 -2.2
2,793,395 + RALBFv3_RS12675 0.64 -2.0
2,793,395 + RALBFv3_RS12675 0.64 -1.6
2,793,395 + RALBFv3_RS12675 0.64 -1.3
2,793,542 + RALBFv3_RS12675 0.79 -2.5
2,793,542 + RALBFv3_RS12675 0.79 -2.3
2,793,599 + RALBFv3_RS12675 0.85 -2.3
2,793,599 + RALBFv3_RS12675 0.85 -2.6
2,793,600 - RALBFv3_RS12675 0.85 -1.0
2,793,687 - -2.7
2,793,687 - -1.0

Or see this region's nucleotide sequence