Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS12655

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS12645 and RALBFv3_RS12650 overlap by 11 nucleotidesRALBFv3_RS12650 and RALBFv3_RS12655 are separated by 10 nucleotidesRALBFv3_RS12655 and RALBFv3_RS12660 overlap by 4 nucleotidesRALBFv3_RS12660 and RALBFv3_RS12665 are separated by 153 nucleotides RALBFv3_RS12645: RALBFv3_RS12645 - dTDP-glucose 4,6-dehydratase, at 2,786,900 to 2,787,964 _RS12645 RALBFv3_RS12650: RALBFv3_RS12650 - dTDP-4-dehydrorhamnose reductase, at 2,787,954 to 2,788,871 _RS12650 RALBFv3_RS12655: RALBFv3_RS12655 - glucose-1-phosphate thymidylyltransferase, at 2,788,882 to 2,789,760 _RS12655 RALBFv3_RS12660: RALBFv3_RS12660 - dTDP-4-dehydrorhamnose 3,5-epimerase, at 2,789,757 to 2,790,308 _RS12660 RALBFv3_RS12665: RALBFv3_RS12665 - Oligosaccharide translocase (flippase), at 2,790,462 to 2,791,730 _RS12665 Position (kb) 2788 2789 2790Strain fitness (log2 ratio) -3 -2 -1 0 1at 2787.953 kb on + strandat 2787.953 kb on + strandat 2788.615 kb on - strand, within RALBFv3_RS12650at 2788.987 kb on + strand, within RALBFv3_RS12655at 2788.987 kb on + strand, within RALBFv3_RS12655at 2788.988 kb on - strand, within RALBFv3_RS12655at 2788.988 kb on - strand, within RALBFv3_RS12655at 2788.988 kb on - strand, within RALBFv3_RS12655at 2789.113 kb on + strand, within RALBFv3_RS12655at 2789.387 kb on - strand, within RALBFv3_RS12655at 2789.387 kb on - strand, within RALBFv3_RS12655at 2789.494 kb on + strand, within RALBFv3_RS12655at 2789.726 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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2,787,953 + -1.0
2,787,953 + -2.1
2,788,615 - RALBFv3_RS12650 0.72 -0.2
2,788,987 + RALBFv3_RS12655 0.12 -1.8
2,788,987 + RALBFv3_RS12655 0.12 -3.2
2,788,988 - RALBFv3_RS12655 0.12 -2.6
2,788,988 - RALBFv3_RS12655 0.12 -2.2
2,788,988 - RALBFv3_RS12655 0.12 -2.4
2,789,113 + RALBFv3_RS12655 0.26 -1.4
2,789,387 - RALBFv3_RS12655 0.57 -1.6
2,789,387 - RALBFv3_RS12655 0.57 -1.0
2,789,494 + RALBFv3_RS12655 0.70 -2.2
2,789,726 - -0.6

Or see this region's nucleotide sequence