Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS12145 and RALBFv3_RS12150 are separated by 49 nucleotides RALBFv3_RS12150 and RALBFv3_RS12155 are separated by 24 nucleotides RALBFv3_RS12155 and RALBFv3_RS12160 are separated by 280 nucleotides
RALBFv3_RS12145: RALBFv3_RS12145 - lipopolysaccharide heptosyltransferase II, at 2,677,777 to 2,678,802
_RS12145
RALBFv3_RS12150: RALBFv3_RS12150 - zinc-finger domain-containing protein, at 2,678,852 to 2,679,049
_RS12150
RALBFv3_RS12155: RALBFv3_RS12155 - branched-chain amino acid transaminase, at 2,679,074 to 2,680,003
_RS12155
RALBFv3_RS12160: RALBFv3_RS12160 - branched-chain amino acid ABC transporter permease, at 2,680,284 to 2,681,081
_RS12160
Position (kb)
2679
2680
2681 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2678.237 kb on + strand, within RALBFv3_RS12145 at 2678.238 kb on - strand, within RALBFv3_RS12145 at 2678.238 kb on - strand, within RALBFv3_RS12145 at 2678.390 kb on + strand, within RALBFv3_RS12145 at 2678.390 kb on + strand, within RALBFv3_RS12145 at 2678.390 kb on + strand, within RALBFv3_RS12145 at 2678.474 kb on + strand, within RALBFv3_RS12145 at 2678.474 kb on + strand, within RALBFv3_RS12145 at 2678.475 kb on - strand, within RALBFv3_RS12145 at 2678.475 kb on - strand, within RALBFv3_RS12145 at 2678.543 kb on + strand, within RALBFv3_RS12145 at 2678.543 kb on + strand, within RALBFv3_RS12145 at 2678.544 kb on - strand, within RALBFv3_RS12145 at 2678.544 kb on - strand, within RALBFv3_RS12145 at 2678.544 kb on - strand, within RALBFv3_RS12145 at 2678.544 kb on - strand, within RALBFv3_RS12145 at 2678.834 kb on - strand at 2678.834 kb on - strand at 2678.892 kb on + strand, within RALBFv3_RS12150 at 2678.893 kb on - strand, within RALBFv3_RS12150 at 2678.893 kb on - strand, within RALBFv3_RS12150 at 2678.961 kb on + strand, within RALBFv3_RS12150 at 2678.961 kb on + strand, within RALBFv3_RS12150 at 2678.961 kb on + strand, within RALBFv3_RS12150 at 2678.961 kb on + strand, within RALBFv3_RS12150 at 2678.962 kb on - strand, within RALBFv3_RS12150 at 2678.962 kb on - strand, within RALBFv3_RS12150 at 2678.962 kb on - strand, within RALBFv3_RS12150 at 2678.962 kb on - strand, within RALBFv3_RS12150 at 2678.962 kb on - strand, within RALBFv3_RS12150 at 2678.962 kb on - strand, within RALBFv3_RS12150 at 2679.099 kb on + strand at 2679.099 kb on + strand at 2679.099 kb on + strand at 2679.099 kb on + strand at 2679.100 kb on - strand at 2679.100 kb on - strand at 2679.100 kb on - strand at 2679.261 kb on + strand, within RALBFv3_RS12155 at 2679.261 kb on + strand, within RALBFv3_RS12155 at 2679.261 kb on + strand, within RALBFv3_RS12155 at 2679.261 kb on + strand, within RALBFv3_RS12155 at 2679.261 kb on + strand, within RALBFv3_RS12155 at 2679.262 kb on - strand, within RALBFv3_RS12155 at 2679.262 kb on - strand, within RALBFv3_RS12155 at 2679.375 kb on + strand, within RALBFv3_RS12155 at 2679.375 kb on + strand, within RALBFv3_RS12155 at 2679.375 kb on + strand, within RALBFv3_RS12155 at 2679.376 kb on - strand, within RALBFv3_RS12155 at 2679.376 kb on - strand, within RALBFv3_RS12155 at 2679.430 kb on - strand, within RALBFv3_RS12155 at 2679.430 kb on - strand, within RALBFv3_RS12155 at 2679.463 kb on - strand, within RALBFv3_RS12155 at 2679.507 kb on + strand, within RALBFv3_RS12155 at 2679.507 kb on + strand, within RALBFv3_RS12155 at 2679.508 kb on - strand, within RALBFv3_RS12155 at 2679.508 kb on - strand, within RALBFv3_RS12155 at 2679.508 kb on - strand, within RALBFv3_RS12155 at 2679.508 kb on - strand, within RALBFv3_RS12155 at 2679.508 kb on - strand, within RALBFv3_RS12155 at 2679.511 kb on - strand, within RALBFv3_RS12155 at 2679.530 kb on + strand, within RALBFv3_RS12155 at 2679.612 kb on + strand at 2679.613 kb on - strand, within RALBFv3_RS12155 at 2679.695 kb on + strand, within RALBFv3_RS12155 at 2679.711 kb on + strand, within RALBFv3_RS12155 at 2679.712 kb on - strand, within RALBFv3_RS12155 at 2679.712 kb on - strand, within RALBFv3_RS12155 at 2679.712 kb on - strand, within RALBFv3_RS12155 at 2679.712 kb on - strand, within RALBFv3_RS12155 at 2679.712 kb on - strand, within RALBFv3_RS12155 at 2679.712 kb on - strand, within RALBFv3_RS12155 at 2679.712 kb on - strand, within RALBFv3_RS12155 at 2679.870 kb on + strand at 2679.871 kb on - strand, within RALBFv3_RS12155 at 2679.871 kb on - strand, within RALBFv3_RS12155 at 2679.871 kb on - strand, within RALBFv3_RS12155 at 2679.903 kb on + strand, within RALBFv3_RS12155 at 2679.903 kb on + strand, within RALBFv3_RS12155 at 2679.904 kb on - strand, within RALBFv3_RS12155 at 2680.054 kb on - strand at 2680.139 kb on + strand at 2680.140 kb on - strand at 2680.151 kb on + strand at 2680.151 kb on + strand at 2680.151 kb on + strand at 2680.163 kb on + strand at 2680.163 kb on + strand at 2680.164 kb on - strand at 2680.165 kb on - strand at 2680.166 kb on - strand at 2680.377 kb on + strand, within RALBFv3_RS12160 at 2680.377 kb on + strand, within RALBFv3_RS12160 at 2680.377 kb on + strand, within RALBFv3_RS12160 at 2680.377 kb on + strand, within RALBFv3_RS12160 at 2680.377 kb on + strand, within RALBFv3_RS12160 at 2680.377 kb on + strand, within RALBFv3_RS12160 at 2680.377 kb on + strand, within RALBFv3_RS12160 at 2680.377 kb on + strand, within RALBFv3_RS12160 at 2680.378 kb on + strand, within RALBFv3_RS12160 at 2680.378 kb on - strand, within RALBFv3_RS12160 at 2680.378 kb on - strand, within RALBFv3_RS12160 at 2680.378 kb on - strand, within RALBFv3_RS12160 at 2680.378 kb on - strand, within RALBFv3_RS12160 at 2680.378 kb on - strand, within RALBFv3_RS12160 at 2680.378 kb on - strand, within RALBFv3_RS12160 at 2680.378 kb on - strand, within RALBFv3_RS12160 at 2680.485 kb on + strand, within RALBFv3_RS12160 at 2680.485 kb on + strand, within RALBFv3_RS12160 at 2680.486 kb on - strand, within RALBFv3_RS12160 at 2680.486 kb on - strand, within RALBFv3_RS12160 at 2680.731 kb on + strand, within RALBFv3_RS12160 at 2680.731 kb on + strand, within RALBFv3_RS12160 at 2680.731 kb on + strand, within RALBFv3_RS12160 at 2680.731 kb on + strand, within RALBFv3_RS12160 at 2680.731 kb on + strand, within RALBFv3_RS12160 at 2680.732 kb on - strand, within RALBFv3_RS12160 at 2680.732 kb on - strand, within RALBFv3_RS12160 at 2680.732 kb on - strand, within RALBFv3_RS12160
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 2,678,237 + RALBFv3_RS12145 0.45 -1.8 2,678,238 - RALBFv3_RS12145 0.45 -1.0 2,678,238 - RALBFv3_RS12145 0.45 -2.4 2,678,390 + RALBFv3_RS12145 0.60 -2.0 2,678,390 + RALBFv3_RS12145 0.60 -1.6 2,678,390 + RALBFv3_RS12145 0.60 -2.3 2,678,474 + RALBFv3_RS12145 0.68 +1.0 2,678,474 + RALBFv3_RS12145 0.68 -2.3 2,678,475 - RALBFv3_RS12145 0.68 +0.0 2,678,475 - RALBFv3_RS12145 0.68 -0.6 2,678,543 + RALBFv3_RS12145 0.75 +1.0 2,678,543 + RALBFv3_RS12145 0.75 -0.6 2,678,544 - RALBFv3_RS12145 0.75 -1.8 2,678,544 - RALBFv3_RS12145 0.75 -1.3 2,678,544 - RALBFv3_RS12145 0.75 -0.6 2,678,544 - RALBFv3_RS12145 0.75 -1.0 2,678,834 - +0.3 2,678,834 - +1.0 2,678,892 + RALBFv3_RS12150 0.20 -3.0 2,678,893 - RALBFv3_RS12150 0.21 +0.0 2,678,893 - RALBFv3_RS12150 0.21 -1.3 2,678,961 + RALBFv3_RS12150 0.55 -0.6 2,678,961 + RALBFv3_RS12150 0.55 -4.1 2,678,961 + RALBFv3_RS12150 0.55 -2.8 2,678,961 + RALBFv3_RS12150 0.55 -1.3 2,678,962 - RALBFv3_RS12150 0.56 -1.0 2,678,962 - RALBFv3_RS12150 0.56 +0.0 2,678,962 - RALBFv3_RS12150 0.56 -0.6 2,678,962 - RALBFv3_RS12150 0.56 -0.3 2,678,962 - RALBFv3_RS12150 0.56 +0.4 2,678,962 - RALBFv3_RS12150 0.56 +1.0 2,679,099 + -0.6 2,679,099 + -3.4 2,679,099 + +0.0 2,679,099 + -2.7 2,679,100 - +0.0 2,679,100 - +0.4 2,679,100 - -0.0 2,679,261 + RALBFv3_RS12155 0.20 -2.0 2,679,261 + RALBFv3_RS12155 0.20 -1.8 2,679,261 + RALBFv3_RS12155 0.20 -4.3 2,679,261 + RALBFv3_RS12155 0.20 -3.4 2,679,261 + RALBFv3_RS12155 0.20 -1.4 2,679,262 - RALBFv3_RS12155 0.20 +0.0 2,679,262 - RALBFv3_RS12155 0.20 +0.4 2,679,375 + RALBFv3_RS12155 0.32 -2.6 2,679,375 + RALBFv3_RS12155 0.32 -2.3 2,679,375 + RALBFv3_RS12155 0.32 -1.3 2,679,376 - RALBFv3_RS12155 0.32 +0.6 2,679,376 - RALBFv3_RS12155 0.32 -0.2 2,679,430 - RALBFv3_RS12155 0.38 +0.6 2,679,430 - RALBFv3_RS12155 0.38 -0.6 2,679,463 - RALBFv3_RS12155 0.42 +0.3 2,679,507 + RALBFv3_RS12155 0.47 +1.0 2,679,507 + RALBFv3_RS12155 0.47 -2.0 2,679,508 - RALBFv3_RS12155 0.47 -0.4 2,679,508 - RALBFv3_RS12155 0.47 +0.2 2,679,508 - RALBFv3_RS12155 0.47 +1.0 2,679,508 - RALBFv3_RS12155 0.47 +0.3 2,679,508 - RALBFv3_RS12155 0.47 -0.1 2,679,511 - RALBFv3_RS12155 0.47 +1.0 2,679,530 + RALBFv3_RS12155 0.49 -3.4 2,679,612 + +0.0 2,679,613 - RALBFv3_RS12155 0.58 -0.0 2,679,695 + RALBFv3_RS12155 0.67 +0.0 2,679,711 + RALBFv3_RS12155 0.68 -1.3 2,679,712 - RALBFv3_RS12155 0.69 +0.0 2,679,712 - RALBFv3_RS12155 0.69 -0.0 2,679,712 - RALBFv3_RS12155 0.69 -0.0 2,679,712 - RALBFv3_RS12155 0.69 +0.1 2,679,712 - RALBFv3_RS12155 0.69 -1.7 2,679,712 - RALBFv3_RS12155 0.69 +0.1 2,679,712 - RALBFv3_RS12155 0.69 -0.2 2,679,870 + +0.0 2,679,871 - RALBFv3_RS12155 0.86 -0.6 2,679,871 - RALBFv3_RS12155 0.86 +0.7 2,679,871 - RALBFv3_RS12155 0.86 +0.2 2,679,903 + RALBFv3_RS12155 0.89 -2.1 2,679,903 + RALBFv3_RS12155 0.89 -2.7 2,679,904 - RALBFv3_RS12155 0.89 +0.1 2,680,054 - -1.0 2,680,139 + +0.5 2,680,140 - -0.1 2,680,151 + +0.0 2,680,151 + +0.0 2,680,151 + -1.0 2,680,163 + +1.3 2,680,163 + -1.0 2,680,164 - -0.9 2,680,165 - +0.1 2,680,166 - -0.0 2,680,377 + RALBFv3_RS12160 0.12 -0.2 2,680,377 + RALBFv3_RS12160 0.12 +0.6 2,680,377 + RALBFv3_RS12160 0.12 +0.9 2,680,377 + RALBFv3_RS12160 0.12 +1.0 2,680,377 + RALBFv3_RS12160 0.12 -0.1 2,680,377 + RALBFv3_RS12160 0.12 -0.2 2,680,377 + RALBFv3_RS12160 0.12 -1.1 2,680,377 + RALBFv3_RS12160 0.12 +0.0 2,680,378 + RALBFv3_RS12160 0.12 -1.1 2,680,378 - RALBFv3_RS12160 0.12 -0.4 2,680,378 - RALBFv3_RS12160 0.12 -0.0 2,680,378 - RALBFv3_RS12160 0.12 +0.1 2,680,378 - RALBFv3_RS12160 0.12 -0.1 2,680,378 - RALBFv3_RS12160 0.12 +0.4 2,680,378 - RALBFv3_RS12160 0.12 -0.3 2,680,378 - RALBFv3_RS12160 0.12 -0.2 2,680,485 + RALBFv3_RS12160 0.25 -0.4 2,680,485 + RALBFv3_RS12160 0.25 +0.1 2,680,486 - RALBFv3_RS12160 0.25 +0.7 2,680,486 - RALBFv3_RS12160 0.25 -0.1 2,680,731 + RALBFv3_RS12160 0.56 -0.6 2,680,731 + RALBFv3_RS12160 0.56 -0.1 2,680,731 + RALBFv3_RS12160 0.56 +0.3 2,680,731 + RALBFv3_RS12160 0.56 -0.4 2,680,731 + RALBFv3_RS12160 0.56 +1.8 2,680,732 - RALBFv3_RS12160 0.56 -0.6 2,680,732 - RALBFv3_RS12160 0.56 +0.0 2,680,732 - RALBFv3_RS12160 0.56 -0.4
Or see this region's nucleotide sequence