Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS12155

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS12145 and RALBFv3_RS12150 are separated by 49 nucleotidesRALBFv3_RS12150 and RALBFv3_RS12155 are separated by 24 nucleotidesRALBFv3_RS12155 and RALBFv3_RS12160 are separated by 280 nucleotides RALBFv3_RS12145: RALBFv3_RS12145 - lipopolysaccharide heptosyltransferase II, at 2,677,777 to 2,678,802 _RS12145 RALBFv3_RS12150: RALBFv3_RS12150 - zinc-finger domain-containing protein, at 2,678,852 to 2,679,049 _RS12150 RALBFv3_RS12155: RALBFv3_RS12155 - branched-chain amino acid transaminase, at 2,679,074 to 2,680,003 _RS12155 RALBFv3_RS12160: RALBFv3_RS12160 - branched-chain amino acid ABC transporter permease, at 2,680,284 to 2,681,081 _RS12160 Position (kb) 2679 2680 2681Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 2678.237 kb on + strand, within RALBFv3_RS12145at 2678.238 kb on - strand, within RALBFv3_RS12145at 2678.238 kb on - strand, within RALBFv3_RS12145at 2678.390 kb on + strand, within RALBFv3_RS12145at 2678.390 kb on + strand, within RALBFv3_RS12145at 2678.390 kb on + strand, within RALBFv3_RS12145at 2678.474 kb on + strand, within RALBFv3_RS12145at 2678.474 kb on + strand, within RALBFv3_RS12145at 2678.475 kb on - strand, within RALBFv3_RS12145at 2678.475 kb on - strand, within RALBFv3_RS12145at 2678.543 kb on + strand, within RALBFv3_RS12145at 2678.543 kb on + strand, within RALBFv3_RS12145at 2678.544 kb on - strand, within RALBFv3_RS12145at 2678.544 kb on - strand, within RALBFv3_RS12145at 2678.544 kb on - strand, within RALBFv3_RS12145at 2678.544 kb on - strand, within RALBFv3_RS12145at 2678.834 kb on - strandat 2678.834 kb on - strandat 2678.892 kb on + strand, within RALBFv3_RS12150at 2678.893 kb on - strand, within RALBFv3_RS12150at 2678.893 kb on - strand, within RALBFv3_RS12150at 2678.961 kb on + strand, within RALBFv3_RS12150at 2678.961 kb on + strand, within RALBFv3_RS12150at 2678.961 kb on + strand, within RALBFv3_RS12150at 2678.961 kb on + strand, within RALBFv3_RS12150at 2678.962 kb on - strand, within RALBFv3_RS12150at 2678.962 kb on - strand, within RALBFv3_RS12150at 2678.962 kb on - strand, within RALBFv3_RS12150at 2678.962 kb on - strand, within RALBFv3_RS12150at 2678.962 kb on - strand, within RALBFv3_RS12150at 2678.962 kb on - strand, within RALBFv3_RS12150at 2679.099 kb on + strandat 2679.099 kb on + strandat 2679.099 kb on + strandat 2679.099 kb on + strandat 2679.100 kb on - strandat 2679.100 kb on - strandat 2679.100 kb on - strandat 2679.261 kb on + strand, within RALBFv3_RS12155at 2679.261 kb on + strand, within RALBFv3_RS12155at 2679.261 kb on + strand, within RALBFv3_RS12155at 2679.261 kb on + strand, within RALBFv3_RS12155at 2679.261 kb on + strand, within RALBFv3_RS12155at 2679.262 kb on - strand, within RALBFv3_RS12155at 2679.262 kb on - strand, within RALBFv3_RS12155at 2679.375 kb on + strand, within RALBFv3_RS12155at 2679.375 kb on + strand, within RALBFv3_RS12155at 2679.375 kb on + strand, within RALBFv3_RS12155at 2679.376 kb on - strand, within RALBFv3_RS12155at 2679.376 kb on - strand, within RALBFv3_RS12155at 2679.430 kb on - strand, within RALBFv3_RS12155at 2679.430 kb on - strand, within RALBFv3_RS12155at 2679.463 kb on - strand, within RALBFv3_RS12155at 2679.507 kb on + strand, within RALBFv3_RS12155at 2679.507 kb on + strand, within RALBFv3_RS12155at 2679.508 kb on - strand, within RALBFv3_RS12155at 2679.508 kb on - strand, within RALBFv3_RS12155at 2679.508 kb on - strand, within RALBFv3_RS12155at 2679.508 kb on - strand, within RALBFv3_RS12155at 2679.508 kb on - strand, within RALBFv3_RS12155at 2679.511 kb on - strand, within RALBFv3_RS12155at 2679.530 kb on + strand, within RALBFv3_RS12155at 2679.612 kb on + strandat 2679.613 kb on - strand, within RALBFv3_RS12155at 2679.695 kb on + strand, within RALBFv3_RS12155at 2679.711 kb on + strand, within RALBFv3_RS12155at 2679.712 kb on - strand, within RALBFv3_RS12155at 2679.712 kb on - strand, within RALBFv3_RS12155at 2679.712 kb on - strand, within RALBFv3_RS12155at 2679.712 kb on - strand, within RALBFv3_RS12155at 2679.712 kb on - strand, within RALBFv3_RS12155at 2679.712 kb on - strand, within RALBFv3_RS12155at 2679.712 kb on - strand, within RALBFv3_RS12155at 2679.870 kb on + strandat 2679.871 kb on - strand, within RALBFv3_RS12155at 2679.871 kb on - strand, within RALBFv3_RS12155at 2679.871 kb on - strand, within RALBFv3_RS12155at 2679.903 kb on + strand, within RALBFv3_RS12155at 2679.903 kb on + strand, within RALBFv3_RS12155at 2679.904 kb on - strand, within RALBFv3_RS12155at 2680.054 kb on - strandat 2680.139 kb on + strandat 2680.140 kb on - strandat 2680.151 kb on + strandat 2680.151 kb on + strandat 2680.151 kb on + strandat 2680.163 kb on + strandat 2680.163 kb on + strandat 2680.164 kb on - strandat 2680.165 kb on - strandat 2680.166 kb on - strandat 2680.377 kb on + strand, within RALBFv3_RS12160at 2680.377 kb on + strand, within RALBFv3_RS12160at 2680.377 kb on + strand, within RALBFv3_RS12160at 2680.377 kb on + strand, within RALBFv3_RS12160at 2680.377 kb on + strand, within RALBFv3_RS12160at 2680.377 kb on + strand, within RALBFv3_RS12160at 2680.377 kb on + strand, within RALBFv3_RS12160at 2680.377 kb on + strand, within RALBFv3_RS12160at 2680.378 kb on + strand, within RALBFv3_RS12160at 2680.378 kb on - strand, within RALBFv3_RS12160at 2680.378 kb on - strand, within RALBFv3_RS12160at 2680.378 kb on - strand, within RALBFv3_RS12160at 2680.378 kb on - strand, within RALBFv3_RS12160at 2680.378 kb on - strand, within RALBFv3_RS12160at 2680.378 kb on - strand, within RALBFv3_RS12160at 2680.378 kb on - strand, within RALBFv3_RS12160at 2680.485 kb on + strand, within RALBFv3_RS12160at 2680.485 kb on + strand, within RALBFv3_RS12160at 2680.486 kb on - strand, within RALBFv3_RS12160at 2680.486 kb on - strand, within RALBFv3_RS12160at 2680.731 kb on + strand, within RALBFv3_RS12160at 2680.731 kb on + strand, within RALBFv3_RS12160at 2680.731 kb on + strand, within RALBFv3_RS12160at 2680.731 kb on + strand, within RALBFv3_RS12160at 2680.731 kb on + strand, within RALBFv3_RS12160at 2680.732 kb on - strand, within RALBFv3_RS12160at 2680.732 kb on - strand, within RALBFv3_RS12160at 2680.732 kb on - strand, within RALBFv3_RS12160

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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2,678,237 + RALBFv3_RS12145 0.45 -1.8
2,678,238 - RALBFv3_RS12145 0.45 -1.0
2,678,238 - RALBFv3_RS12145 0.45 -2.4
2,678,390 + RALBFv3_RS12145 0.60 -2.0
2,678,390 + RALBFv3_RS12145 0.60 -1.6
2,678,390 + RALBFv3_RS12145 0.60 -2.3
2,678,474 + RALBFv3_RS12145 0.68 +1.0
2,678,474 + RALBFv3_RS12145 0.68 -2.3
2,678,475 - RALBFv3_RS12145 0.68 +0.0
2,678,475 - RALBFv3_RS12145 0.68 -0.6
2,678,543 + RALBFv3_RS12145 0.75 +1.0
2,678,543 + RALBFv3_RS12145 0.75 -0.6
2,678,544 - RALBFv3_RS12145 0.75 -1.8
2,678,544 - RALBFv3_RS12145 0.75 -1.3
2,678,544 - RALBFv3_RS12145 0.75 -0.6
2,678,544 - RALBFv3_RS12145 0.75 -1.0
2,678,834 - +0.3
2,678,834 - +1.0
2,678,892 + RALBFv3_RS12150 0.20 -3.0
2,678,893 - RALBFv3_RS12150 0.21 +0.0
2,678,893 - RALBFv3_RS12150 0.21 -1.3
2,678,961 + RALBFv3_RS12150 0.55 -0.6
2,678,961 + RALBFv3_RS12150 0.55 -4.1
2,678,961 + RALBFv3_RS12150 0.55 -2.8
2,678,961 + RALBFv3_RS12150 0.55 -1.3
2,678,962 - RALBFv3_RS12150 0.56 -1.0
2,678,962 - RALBFv3_RS12150 0.56 +0.0
2,678,962 - RALBFv3_RS12150 0.56 -0.6
2,678,962 - RALBFv3_RS12150 0.56 -0.3
2,678,962 - RALBFv3_RS12150 0.56 +0.4
2,678,962 - RALBFv3_RS12150 0.56 +1.0
2,679,099 + -0.6
2,679,099 + -3.4
2,679,099 + +0.0
2,679,099 + -2.7
2,679,100 - +0.0
2,679,100 - +0.4
2,679,100 - -0.0
2,679,261 + RALBFv3_RS12155 0.20 -2.0
2,679,261 + RALBFv3_RS12155 0.20 -1.8
2,679,261 + RALBFv3_RS12155 0.20 -4.3
2,679,261 + RALBFv3_RS12155 0.20 -3.4
2,679,261 + RALBFv3_RS12155 0.20 -1.4
2,679,262 - RALBFv3_RS12155 0.20 +0.0
2,679,262 - RALBFv3_RS12155 0.20 +0.4
2,679,375 + RALBFv3_RS12155 0.32 -2.6
2,679,375 + RALBFv3_RS12155 0.32 -2.3
2,679,375 + RALBFv3_RS12155 0.32 -1.3
2,679,376 - RALBFv3_RS12155 0.32 +0.6
2,679,376 - RALBFv3_RS12155 0.32 -0.2
2,679,430 - RALBFv3_RS12155 0.38 +0.6
2,679,430 - RALBFv3_RS12155 0.38 -0.6
2,679,463 - RALBFv3_RS12155 0.42 +0.3
2,679,507 + RALBFv3_RS12155 0.47 +1.0
2,679,507 + RALBFv3_RS12155 0.47 -2.0
2,679,508 - RALBFv3_RS12155 0.47 -0.4
2,679,508 - RALBFv3_RS12155 0.47 +0.2
2,679,508 - RALBFv3_RS12155 0.47 +1.0
2,679,508 - RALBFv3_RS12155 0.47 +0.3
2,679,508 - RALBFv3_RS12155 0.47 -0.1
2,679,511 - RALBFv3_RS12155 0.47 +1.0
2,679,530 + RALBFv3_RS12155 0.49 -3.4
2,679,612 + +0.0
2,679,613 - RALBFv3_RS12155 0.58 -0.0
2,679,695 + RALBFv3_RS12155 0.67 +0.0
2,679,711 + RALBFv3_RS12155 0.68 -1.3
2,679,712 - RALBFv3_RS12155 0.69 +0.0
2,679,712 - RALBFv3_RS12155 0.69 -0.0
2,679,712 - RALBFv3_RS12155 0.69 -0.0
2,679,712 - RALBFv3_RS12155 0.69 +0.1
2,679,712 - RALBFv3_RS12155 0.69 -1.7
2,679,712 - RALBFv3_RS12155 0.69 +0.1
2,679,712 - RALBFv3_RS12155 0.69 -0.2
2,679,870 + +0.0
2,679,871 - RALBFv3_RS12155 0.86 -0.6
2,679,871 - RALBFv3_RS12155 0.86 +0.7
2,679,871 - RALBFv3_RS12155 0.86 +0.2
2,679,903 + RALBFv3_RS12155 0.89 -2.1
2,679,903 + RALBFv3_RS12155 0.89 -2.7
2,679,904 - RALBFv3_RS12155 0.89 +0.1
2,680,054 - -1.0
2,680,139 + +0.5
2,680,140 - -0.1
2,680,151 + +0.0
2,680,151 + +0.0
2,680,151 + -1.0
2,680,163 + +1.3
2,680,163 + -1.0
2,680,164 - -0.9
2,680,165 - +0.1
2,680,166 - -0.0
2,680,377 + RALBFv3_RS12160 0.12 -0.2
2,680,377 + RALBFv3_RS12160 0.12 +0.6
2,680,377 + RALBFv3_RS12160 0.12 +0.9
2,680,377 + RALBFv3_RS12160 0.12 +1.0
2,680,377 + RALBFv3_RS12160 0.12 -0.1
2,680,377 + RALBFv3_RS12160 0.12 -0.2
2,680,377 + RALBFv3_RS12160 0.12 -1.1
2,680,377 + RALBFv3_RS12160 0.12 +0.0
2,680,378 + RALBFv3_RS12160 0.12 -1.1
2,680,378 - RALBFv3_RS12160 0.12 -0.4
2,680,378 - RALBFv3_RS12160 0.12 -0.0
2,680,378 - RALBFv3_RS12160 0.12 +0.1
2,680,378 - RALBFv3_RS12160 0.12 -0.1
2,680,378 - RALBFv3_RS12160 0.12 +0.4
2,680,378 - RALBFv3_RS12160 0.12 -0.3
2,680,378 - RALBFv3_RS12160 0.12 -0.2
2,680,485 + RALBFv3_RS12160 0.25 -0.4
2,680,485 + RALBFv3_RS12160 0.25 +0.1
2,680,486 - RALBFv3_RS12160 0.25 +0.7
2,680,486 - RALBFv3_RS12160 0.25 -0.1
2,680,731 + RALBFv3_RS12160 0.56 -0.6
2,680,731 + RALBFv3_RS12160 0.56 -0.1
2,680,731 + RALBFv3_RS12160 0.56 +0.3
2,680,731 + RALBFv3_RS12160 0.56 -0.4
2,680,731 + RALBFv3_RS12160 0.56 +1.8
2,680,732 - RALBFv3_RS12160 0.56 -0.6
2,680,732 - RALBFv3_RS12160 0.56 +0.0
2,680,732 - RALBFv3_RS12160 0.56 -0.4

Or see this region's nucleotide sequence