Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS12000 and RALBFv3_RS12005 overlap by 8 nucleotides RALBFv3_RS12005 and RALBFv3_RS12010 are separated by 276 nucleotides RALBFv3_RS12010 and RALBFv3_RS12015 are separated by 94 nucleotides RALBFv3_RS12015 and RALBFv3_RS12020 are separated by 273 nucleotides
RALBFv3_RS12000: RALBFv3_RS12000 - sensor histidine kinase, at 2,647,665 to 2,649,206
_RS12000
RALBFv3_RS12005: RALBFv3_RS12005 - DNA-binding response regulator, at 2,649,199 to 2,649,897
_RS12005
RALBFv3_RS12010: RALBFv3_RS12010 - recombinase RecA, at 2,650,174 to 2,651,232
_RS12010
RALBFv3_RS12015: RALBFv3_RS12015 - recombination regulator RecX, at 2,651,327 to 2,651,806
_RS12015
RALBFv3_RS12020: RALBFv3_RS12020 - DUF2889 domain-containing protein, at 2,652,080 to 2,652,706
_RS12020
Position (kb)
2650
2651
2652 Strain fitness (log2 ratio)
-2
-1
0
1 at 2649.242 kb on + strand at 2649.242 kb on + strand at 2649.311 kb on + strand, within RALBFv3_RS12005 at 2649.311 kb on + strand, within RALBFv3_RS12005 at 2649.311 kb on + strand, within RALBFv3_RS12005 at 2649.311 kb on + strand, within RALBFv3_RS12005 at 2649.312 kb on - strand, within RALBFv3_RS12005 at 2649.312 kb on - strand, within RALBFv3_RS12005 at 2649.312 kb on - strand, within RALBFv3_RS12005 at 2649.312 kb on - strand, within RALBFv3_RS12005 at 2649.312 kb on - strand, within RALBFv3_RS12005 at 2649.312 kb on - strand, within RALBFv3_RS12005 at 2649.312 kb on - strand, within RALBFv3_RS12005 at 2649.464 kb on + strand, within RALBFv3_RS12005 at 2649.464 kb on + strand, within RALBFv3_RS12005 at 2649.464 kb on + strand, within RALBFv3_RS12005 at 2649.464 kb on + strand, within RALBFv3_RS12005 at 2649.465 kb on - strand, within RALBFv3_RS12005 at 2649.465 kb on - strand, within RALBFv3_RS12005 at 2649.465 kb on - strand, within RALBFv3_RS12005 at 2649.465 kb on - strand, within RALBFv3_RS12005 at 2649.596 kb on + strand, within RALBFv3_RS12005 at 2649.596 kb on + strand, within RALBFv3_RS12005 at 2649.596 kb on + strand, within RALBFv3_RS12005 at 2649.596 kb on + strand, within RALBFv3_RS12005 at 2649.596 kb on + strand, within RALBFv3_RS12005 at 2649.597 kb on - strand, within RALBFv3_RS12005 at 2649.597 kb on - strand, within RALBFv3_RS12005 at 2649.597 kb on - strand, within RALBFv3_RS12005 at 2649.597 kb on - strand, within RALBFv3_RS12005 at 2649.597 kb on - strand, within RALBFv3_RS12005 at 2649.597 kb on - strand, within RALBFv3_RS12005 at 2649.597 kb on - strand, within RALBFv3_RS12005 at 2649.754 kb on + strand, within RALBFv3_RS12005 at 2649.755 kb on + strand, within RALBFv3_RS12005 at 2649.756 kb on - strand, within RALBFv3_RS12005 at 2649.756 kb on - strand, within RALBFv3_RS12005 at 2649.951 kb on + strand at 2649.973 kb on + strand at 2650.033 kb on + strand at 2650.033 kb on + strand at 2650.048 kb on + strand at 2650.048 kb on + strand at 2650.053 kb on + strand at 2650.146 kb on + strand at 2650.146 kb on + strand at 2650.147 kb on - strand at 2650.147 kb on - strand at 2650.147 kb on - strand at 2650.148 kb on + strand at 2650.148 kb on + strand at 2650.149 kb on - strand at 2650.390 kb on + strand, within RALBFv3_RS12010 at 2650.391 kb on - strand, within RALBFv3_RS12010 at 2650.391 kb on - strand, within RALBFv3_RS12010 at 2650.391 kb on - strand, within RALBFv3_RS12010 at 2650.391 kb on - strand, within RALBFv3_RS12010 at 2650.473 kb on + strand, within RALBFv3_RS12010 at 2650.473 kb on + strand, within RALBFv3_RS12010 at 2650.849 kb on + strand, within RALBFv3_RS12010 at 2650.849 kb on + strand, within RALBFv3_RS12010 at 2650.849 kb on + strand, within RALBFv3_RS12010 at 2650.850 kb on - strand, within RALBFv3_RS12010 at 2650.850 kb on - strand, within RALBFv3_RS12010 at 2650.850 kb on - strand, within RALBFv3_RS12010 at 2650.987 kb on + strand, within RALBFv3_RS12010 at 2650.988 kb on - strand, within RALBFv3_RS12010 at 2651.033 kb on + strand, within RALBFv3_RS12010 at 2651.034 kb on - strand, within RALBFv3_RS12010 at 2651.034 kb on - strand, within RALBFv3_RS12010 at 2651.074 kb on + strand, within RALBFv3_RS12010 at 2651.074 kb on + strand, within RALBFv3_RS12010 at 2651.074 kb on + strand, within RALBFv3_RS12010 at 2651.075 kb on - strand, within RALBFv3_RS12010 at 2651.075 kb on - strand, within RALBFv3_RS12010 at 2651.075 kb on - strand, within RALBFv3_RS12010 at 2651.075 kb on - strand, within RALBFv3_RS12010 at 2651.075 kb on - strand, within RALBFv3_RS12010 at 2651.299 kb on + strand at 2651.299 kb on + strand at 2651.300 kb on - strand at 2651.300 kb on - strand at 2651.375 kb on + strand, within RALBFv3_RS12015 at 2651.376 kb on - strand, within RALBFv3_RS12015 at 2651.540 kb on + strand, within RALBFv3_RS12015 at 2651.853 kb on + strand at 2651.854 kb on - strand at 2651.854 kb on - strand at 2651.854 kb on - strand at 2651.854 kb on - strand at 2651.854 kb on - strand at 2651.854 kb on - strand at 2651.854 kb on - strand at 2651.855 kb on + strand at 2651.855 kb on + strand at 2651.855 kb on + strand at 2651.856 kb on - strand at 2651.856 kb on - strand at 2651.856 kb on - strand at 2651.943 kb on + strand at 2651.943 kb on + strand at 2651.943 kb on + strand at 2651.944 kb on - strand at 2651.964 kb on + strand at 2651.965 kb on - strand at 2651.965 kb on - strand at 2651.965 kb on - strand at 2651.991 kb on + strand at 2652.063 kb on - strand at 2652.063 kb on - strand at 2652.143 kb on + strand, within RALBFv3_RS12020 at 2652.143 kb on + strand, within RALBFv3_RS12020 at 2652.143 kb on + strand, within RALBFv3_RS12020 at 2652.143 kb on + strand, within RALBFv3_RS12020 at 2652.144 kb on - strand, within RALBFv3_RS12020 at 2652.144 kb on - strand, within RALBFv3_RS12020 at 2652.144 kb on - strand, within RALBFv3_RS12020 at 2652.226 kb on + strand, within RALBFv3_RS12020 at 2652.226 kb on + strand, within RALBFv3_RS12020 at 2652.226 kb on + strand, within RALBFv3_RS12020
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 2,649,242 + +0.0 2,649,242 + +1.2 2,649,311 + RALBFv3_RS12005 0.16 -0.0 2,649,311 + RALBFv3_RS12005 0.16 -0.2 2,649,311 + RALBFv3_RS12005 0.16 +0.3 2,649,311 + RALBFv3_RS12005 0.16 +1.0 2,649,312 - RALBFv3_RS12005 0.16 -0.2 2,649,312 - RALBFv3_RS12005 0.16 -0.3 2,649,312 - RALBFv3_RS12005 0.16 +1.0 2,649,312 - RALBFv3_RS12005 0.16 +0.3 2,649,312 - RALBFv3_RS12005 0.16 +1.0 2,649,312 - RALBFv3_RS12005 0.16 +0.6 2,649,312 - RALBFv3_RS12005 0.16 +0.4 2,649,464 + RALBFv3_RS12005 0.38 +0.3 2,649,464 + RALBFv3_RS12005 0.38 -0.1 2,649,464 + RALBFv3_RS12005 0.38 -0.1 2,649,464 + RALBFv3_RS12005 0.38 -0.1 2,649,465 - RALBFv3_RS12005 0.38 -0.4 2,649,465 - RALBFv3_RS12005 0.38 -0.1 2,649,465 - RALBFv3_RS12005 0.38 +0.9 2,649,465 - RALBFv3_RS12005 0.38 -0.4 2,649,596 + RALBFv3_RS12005 0.57 -1.5 2,649,596 + RALBFv3_RS12005 0.57 -0.3 2,649,596 + RALBFv3_RS12005 0.57 +0.1 2,649,596 + RALBFv3_RS12005 0.57 +0.5 2,649,596 + RALBFv3_RS12005 0.57 -0.1 2,649,597 - RALBFv3_RS12005 0.57 -1.0 2,649,597 - RALBFv3_RS12005 0.57 -0.0 2,649,597 - RALBFv3_RS12005 0.57 -1.1 2,649,597 - RALBFv3_RS12005 0.57 +0.5 2,649,597 - RALBFv3_RS12005 0.57 +0.2 2,649,597 - RALBFv3_RS12005 0.57 -0.1 2,649,597 - RALBFv3_RS12005 0.57 -1.1 2,649,754 + RALBFv3_RS12005 0.79 +1.0 2,649,755 + RALBFv3_RS12005 0.80 -0.1 2,649,756 - RALBFv3_RS12005 0.80 +0.0 2,649,756 - RALBFv3_RS12005 0.80 +0.3 2,649,951 + -0.3 2,649,973 + -0.3 2,650,033 + +0.1 2,650,033 + -0.3 2,650,048 + +0.3 2,650,048 + -0.6 2,650,053 + +0.7 2,650,146 + +0.0 2,650,146 + -0.3 2,650,147 - -1.5 2,650,147 - -2.3 2,650,147 - +0.0 2,650,148 + +0.6 2,650,148 + -1.4 2,650,149 - -1.5 2,650,390 + RALBFv3_RS12010 0.20 +1.0 2,650,391 - RALBFv3_RS12010 0.20 -1.3 2,650,391 - RALBFv3_RS12010 0.20 -0.5 2,650,391 - RALBFv3_RS12010 0.20 +0.0 2,650,391 - RALBFv3_RS12010 0.20 +1.0 2,650,473 + RALBFv3_RS12010 0.28 -0.8 2,650,473 + RALBFv3_RS12010 0.28 -2.7 2,650,849 + RALBFv3_RS12010 0.64 -1.0 2,650,849 + RALBFv3_RS12010 0.64 +0.2 2,650,849 + RALBFv3_RS12010 0.64 -1.3 2,650,850 - RALBFv3_RS12010 0.64 -1.1 2,650,850 - RALBFv3_RS12010 0.64 -1.3 2,650,850 - RALBFv3_RS12010 0.64 -0.5 2,650,987 + RALBFv3_RS12010 0.77 -0.9 2,650,988 - RALBFv3_RS12010 0.77 -0.5 2,651,033 + RALBFv3_RS12010 0.81 +0.0 2,651,034 - RALBFv3_RS12010 0.81 +1.0 2,651,034 - RALBFv3_RS12010 0.81 -0.5 2,651,074 + RALBFv3_RS12010 0.85 +1.0 2,651,074 + RALBFv3_RS12010 0.85 +1.0 2,651,074 + RALBFv3_RS12010 0.85 +0.0 2,651,075 - RALBFv3_RS12010 0.85 -1.8 2,651,075 - RALBFv3_RS12010 0.85 -2.7 2,651,075 - RALBFv3_RS12010 0.85 +0.0 2,651,075 - RALBFv3_RS12010 0.85 +1.0 2,651,075 - RALBFv3_RS12010 0.85 -1.3 2,651,299 + +0.0 2,651,299 + +1.0 2,651,300 - +0.6 2,651,300 - -0.0 2,651,375 + RALBFv3_RS12015 0.10 -0.1 2,651,376 - RALBFv3_RS12015 0.10 +1.0 2,651,540 + RALBFv3_RS12015 0.44 -0.7 2,651,853 + -0.1 2,651,854 - -0.0 2,651,854 - -0.3 2,651,854 - -0.6 2,651,854 - -1.2 2,651,854 - +0.3 2,651,854 - +1.0 2,651,854 - -1.3 2,651,855 + +1.3 2,651,855 + +1.5 2,651,855 + +0.8 2,651,856 - +0.7 2,651,856 - +1.0 2,651,856 - +0.4 2,651,943 + +0.2 2,651,943 + -0.5 2,651,943 + +1.0 2,651,944 - +0.5 2,651,964 + -1.0 2,651,965 - +1.2 2,651,965 - +0.4 2,651,965 - +0.8 2,651,991 + +0.2 2,652,063 - +0.4 2,652,063 - +0.9 2,652,143 + RALBFv3_RS12020 0.10 -0.2 2,652,143 + RALBFv3_RS12020 0.10 +0.4 2,652,143 + RALBFv3_RS12020 0.10 -0.5 2,652,143 + RALBFv3_RS12020 0.10 +0.7 2,652,144 - RALBFv3_RS12020 0.10 -0.2 2,652,144 - RALBFv3_RS12020 0.10 +1.5 2,652,144 - RALBFv3_RS12020 0.10 +1.3 2,652,226 + RALBFv3_RS12020 0.23 -0.4 2,652,226 + RALBFv3_RS12020 0.23 +0.0 2,652,226 + RALBFv3_RS12020 0.23 -0.5
Or see this region's nucleotide sequence