Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS12010

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS12000 and RALBFv3_RS12005 overlap by 8 nucleotidesRALBFv3_RS12005 and RALBFv3_RS12010 are separated by 276 nucleotidesRALBFv3_RS12010 and RALBFv3_RS12015 are separated by 94 nucleotidesRALBFv3_RS12015 and RALBFv3_RS12020 are separated by 273 nucleotides RALBFv3_RS12000: RALBFv3_RS12000 - sensor histidine kinase, at 2,647,665 to 2,649,206 _RS12000 RALBFv3_RS12005: RALBFv3_RS12005 - DNA-binding response regulator, at 2,649,199 to 2,649,897 _RS12005 RALBFv3_RS12010: RALBFv3_RS12010 - recombinase RecA, at 2,650,174 to 2,651,232 _RS12010 RALBFv3_RS12015: RALBFv3_RS12015 - recombination regulator RecX, at 2,651,327 to 2,651,806 _RS12015 RALBFv3_RS12020: RALBFv3_RS12020 - DUF2889 domain-containing protein, at 2,652,080 to 2,652,706 _RS12020 Position (kb) 2650 2651 2652Strain fitness (log2 ratio) -2 -1 0 1at 2649.242 kb on + strandat 2649.242 kb on + strandat 2649.311 kb on + strand, within RALBFv3_RS12005at 2649.311 kb on + strand, within RALBFv3_RS12005at 2649.311 kb on + strand, within RALBFv3_RS12005at 2649.311 kb on + strand, within RALBFv3_RS12005at 2649.312 kb on - strand, within RALBFv3_RS12005at 2649.312 kb on - strand, within RALBFv3_RS12005at 2649.312 kb on - strand, within RALBFv3_RS12005at 2649.312 kb on - strand, within RALBFv3_RS12005at 2649.312 kb on - strand, within RALBFv3_RS12005at 2649.312 kb on - strand, within RALBFv3_RS12005at 2649.312 kb on - strand, within RALBFv3_RS12005at 2649.464 kb on + strand, within RALBFv3_RS12005at 2649.464 kb on + strand, within RALBFv3_RS12005at 2649.464 kb on + strand, within RALBFv3_RS12005at 2649.464 kb on + strand, within RALBFv3_RS12005at 2649.465 kb on - strand, within RALBFv3_RS12005at 2649.465 kb on - strand, within RALBFv3_RS12005at 2649.465 kb on - strand, within RALBFv3_RS12005at 2649.465 kb on - strand, within RALBFv3_RS12005at 2649.596 kb on + strand, within RALBFv3_RS12005at 2649.596 kb on + strand, within RALBFv3_RS12005at 2649.596 kb on + strand, within RALBFv3_RS12005at 2649.596 kb on + strand, within RALBFv3_RS12005at 2649.596 kb on + strand, within RALBFv3_RS12005at 2649.597 kb on - strand, within RALBFv3_RS12005at 2649.597 kb on - strand, within RALBFv3_RS12005at 2649.597 kb on - strand, within RALBFv3_RS12005at 2649.597 kb on - strand, within RALBFv3_RS12005at 2649.597 kb on - strand, within RALBFv3_RS12005at 2649.597 kb on - strand, within RALBFv3_RS12005at 2649.597 kb on - strand, within RALBFv3_RS12005at 2649.754 kb on + strand, within RALBFv3_RS12005at 2649.755 kb on + strand, within RALBFv3_RS12005at 2649.756 kb on - strand, within RALBFv3_RS12005at 2649.756 kb on - strand, within RALBFv3_RS12005at 2649.951 kb on + strandat 2649.973 kb on + strandat 2650.033 kb on + strandat 2650.033 kb on + strandat 2650.048 kb on + strandat 2650.048 kb on + strandat 2650.053 kb on + strandat 2650.146 kb on + strandat 2650.146 kb on + strandat 2650.147 kb on - strandat 2650.147 kb on - strandat 2650.147 kb on - strandat 2650.148 kb on + strandat 2650.148 kb on + strandat 2650.149 kb on - strandat 2650.390 kb on + strand, within RALBFv3_RS12010at 2650.391 kb on - strand, within RALBFv3_RS12010at 2650.391 kb on - strand, within RALBFv3_RS12010at 2650.391 kb on - strand, within RALBFv3_RS12010at 2650.391 kb on - strand, within RALBFv3_RS12010at 2650.473 kb on + strand, within RALBFv3_RS12010at 2650.473 kb on + strand, within RALBFv3_RS12010at 2650.849 kb on + strand, within RALBFv3_RS12010at 2650.849 kb on + strand, within RALBFv3_RS12010at 2650.849 kb on + strand, within RALBFv3_RS12010at 2650.850 kb on - strand, within RALBFv3_RS12010at 2650.850 kb on - strand, within RALBFv3_RS12010at 2650.850 kb on - strand, within RALBFv3_RS12010at 2650.987 kb on + strand, within RALBFv3_RS12010at 2650.988 kb on - strand, within RALBFv3_RS12010at 2651.033 kb on + strand, within RALBFv3_RS12010at 2651.034 kb on - strand, within RALBFv3_RS12010at 2651.034 kb on - strand, within RALBFv3_RS12010at 2651.074 kb on + strand, within RALBFv3_RS12010at 2651.074 kb on + strand, within RALBFv3_RS12010at 2651.074 kb on + strand, within RALBFv3_RS12010at 2651.075 kb on - strand, within RALBFv3_RS12010at 2651.075 kb on - strand, within RALBFv3_RS12010at 2651.075 kb on - strand, within RALBFv3_RS12010at 2651.075 kb on - strand, within RALBFv3_RS12010at 2651.075 kb on - strand, within RALBFv3_RS12010at 2651.299 kb on + strandat 2651.299 kb on + strandat 2651.300 kb on - strandat 2651.300 kb on - strandat 2651.375 kb on + strand, within RALBFv3_RS12015at 2651.376 kb on - strand, within RALBFv3_RS12015at 2651.540 kb on + strand, within RALBFv3_RS12015at 2651.853 kb on + strandat 2651.854 kb on - strandat 2651.854 kb on - strandat 2651.854 kb on - strandat 2651.854 kb on - strandat 2651.854 kb on - strandat 2651.854 kb on - strandat 2651.854 kb on - strandat 2651.855 kb on + strandat 2651.855 kb on + strandat 2651.855 kb on + strandat 2651.856 kb on - strandat 2651.856 kb on - strandat 2651.856 kb on - strandat 2651.943 kb on + strandat 2651.943 kb on + strandat 2651.943 kb on + strandat 2651.944 kb on - strandat 2651.964 kb on + strandat 2651.965 kb on - strandat 2651.965 kb on - strandat 2651.965 kb on - strandat 2651.991 kb on + strandat 2652.063 kb on - strandat 2652.063 kb on - strandat 2652.143 kb on + strand, within RALBFv3_RS12020at 2652.143 kb on + strand, within RALBFv3_RS12020at 2652.143 kb on + strand, within RALBFv3_RS12020at 2652.143 kb on + strand, within RALBFv3_RS12020at 2652.144 kb on - strand, within RALBFv3_RS12020at 2652.144 kb on - strand, within RALBFv3_RS12020at 2652.144 kb on - strand, within RALBFv3_RS12020at 2652.226 kb on + strand, within RALBFv3_RS12020at 2652.226 kb on + strand, within RALBFv3_RS12020at 2652.226 kb on + strand, within RALBFv3_RS12020

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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2,649,242 + +0.0
2,649,242 + +1.2
2,649,311 + RALBFv3_RS12005 0.16 -0.0
2,649,311 + RALBFv3_RS12005 0.16 -0.2
2,649,311 + RALBFv3_RS12005 0.16 +0.3
2,649,311 + RALBFv3_RS12005 0.16 +1.0
2,649,312 - RALBFv3_RS12005 0.16 -0.2
2,649,312 - RALBFv3_RS12005 0.16 -0.3
2,649,312 - RALBFv3_RS12005 0.16 +1.0
2,649,312 - RALBFv3_RS12005 0.16 +0.3
2,649,312 - RALBFv3_RS12005 0.16 +1.0
2,649,312 - RALBFv3_RS12005 0.16 +0.6
2,649,312 - RALBFv3_RS12005 0.16 +0.4
2,649,464 + RALBFv3_RS12005 0.38 +0.3
2,649,464 + RALBFv3_RS12005 0.38 -0.1
2,649,464 + RALBFv3_RS12005 0.38 -0.1
2,649,464 + RALBFv3_RS12005 0.38 -0.1
2,649,465 - RALBFv3_RS12005 0.38 -0.4
2,649,465 - RALBFv3_RS12005 0.38 -0.1
2,649,465 - RALBFv3_RS12005 0.38 +0.9
2,649,465 - RALBFv3_RS12005 0.38 -0.4
2,649,596 + RALBFv3_RS12005 0.57 -1.5
2,649,596 + RALBFv3_RS12005 0.57 -0.3
2,649,596 + RALBFv3_RS12005 0.57 +0.1
2,649,596 + RALBFv3_RS12005 0.57 +0.5
2,649,596 + RALBFv3_RS12005 0.57 -0.1
2,649,597 - RALBFv3_RS12005 0.57 -1.0
2,649,597 - RALBFv3_RS12005 0.57 -0.0
2,649,597 - RALBFv3_RS12005 0.57 -1.1
2,649,597 - RALBFv3_RS12005 0.57 +0.5
2,649,597 - RALBFv3_RS12005 0.57 +0.2
2,649,597 - RALBFv3_RS12005 0.57 -0.1
2,649,597 - RALBFv3_RS12005 0.57 -1.1
2,649,754 + RALBFv3_RS12005 0.79 +1.0
2,649,755 + RALBFv3_RS12005 0.80 -0.1
2,649,756 - RALBFv3_RS12005 0.80 +0.0
2,649,756 - RALBFv3_RS12005 0.80 +0.3
2,649,951 + -0.3
2,649,973 + -0.3
2,650,033 + +0.1
2,650,033 + -0.3
2,650,048 + +0.3
2,650,048 + -0.6
2,650,053 + +0.7
2,650,146 + +0.0
2,650,146 + -0.3
2,650,147 - -1.5
2,650,147 - -2.3
2,650,147 - +0.0
2,650,148 + +0.6
2,650,148 + -1.4
2,650,149 - -1.5
2,650,390 + RALBFv3_RS12010 0.20 +1.0
2,650,391 - RALBFv3_RS12010 0.20 -1.3
2,650,391 - RALBFv3_RS12010 0.20 -0.5
2,650,391 - RALBFv3_RS12010 0.20 +0.0
2,650,391 - RALBFv3_RS12010 0.20 +1.0
2,650,473 + RALBFv3_RS12010 0.28 -0.8
2,650,473 + RALBFv3_RS12010 0.28 -2.7
2,650,849 + RALBFv3_RS12010 0.64 -1.0
2,650,849 + RALBFv3_RS12010 0.64 +0.2
2,650,849 + RALBFv3_RS12010 0.64 -1.3
2,650,850 - RALBFv3_RS12010 0.64 -1.1
2,650,850 - RALBFv3_RS12010 0.64 -1.3
2,650,850 - RALBFv3_RS12010 0.64 -0.5
2,650,987 + RALBFv3_RS12010 0.77 -0.9
2,650,988 - RALBFv3_RS12010 0.77 -0.5
2,651,033 + RALBFv3_RS12010 0.81 +0.0
2,651,034 - RALBFv3_RS12010 0.81 +1.0
2,651,034 - RALBFv3_RS12010 0.81 -0.5
2,651,074 + RALBFv3_RS12010 0.85 +1.0
2,651,074 + RALBFv3_RS12010 0.85 +1.0
2,651,074 + RALBFv3_RS12010 0.85 +0.0
2,651,075 - RALBFv3_RS12010 0.85 -1.8
2,651,075 - RALBFv3_RS12010 0.85 -2.7
2,651,075 - RALBFv3_RS12010 0.85 +0.0
2,651,075 - RALBFv3_RS12010 0.85 +1.0
2,651,075 - RALBFv3_RS12010 0.85 -1.3
2,651,299 + +0.0
2,651,299 + +1.0
2,651,300 - +0.6
2,651,300 - -0.0
2,651,375 + RALBFv3_RS12015 0.10 -0.1
2,651,376 - RALBFv3_RS12015 0.10 +1.0
2,651,540 + RALBFv3_RS12015 0.44 -0.7
2,651,853 + -0.1
2,651,854 - -0.0
2,651,854 - -0.3
2,651,854 - -0.6
2,651,854 - -1.2
2,651,854 - +0.3
2,651,854 - +1.0
2,651,854 - -1.3
2,651,855 + +1.3
2,651,855 + +1.5
2,651,855 + +0.8
2,651,856 - +0.7
2,651,856 - +1.0
2,651,856 - +0.4
2,651,943 + +0.2
2,651,943 + -0.5
2,651,943 + +1.0
2,651,944 - +0.5
2,651,964 + -1.0
2,651,965 - +1.2
2,651,965 - +0.4
2,651,965 - +0.8
2,651,991 + +0.2
2,652,063 - +0.4
2,652,063 - +0.9
2,652,143 + RALBFv3_RS12020 0.10 -0.2
2,652,143 + RALBFv3_RS12020 0.10 +0.4
2,652,143 + RALBFv3_RS12020 0.10 -0.5
2,652,143 + RALBFv3_RS12020 0.10 +0.7
2,652,144 - RALBFv3_RS12020 0.10 -0.2
2,652,144 - RALBFv3_RS12020 0.10 +1.5
2,652,144 - RALBFv3_RS12020 0.10 +1.3
2,652,226 + RALBFv3_RS12020 0.23 -0.4
2,652,226 + RALBFv3_RS12020 0.23 +0.0
2,652,226 + RALBFv3_RS12020 0.23 -0.5

Or see this region's nucleotide sequence