Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS11775

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS23950 and RALBFv3_RS11770 are separated by 48 nucleotidesRALBFv3_RS11770 and RALBFv3_RS11775 overlap by 4 nucleotidesRALBFv3_RS11775 and RALBFv3_RS11780 overlap by 4 nucleotidesRALBFv3_RS11780 and RALBFv3_RS11785 are separated by 20 nucleotides RALBFv3_RS23950: RALBFv3_RS23950 - hypothetical protein, at 2,590,893 to 2,591,090 _RS23950 RALBFv3_RS11770: RALBFv3_RS11770 - DUF45 domain-containing protein, at 2,591,139 to 2,592,005 _RS11770 RALBFv3_RS11775: RALBFv3_RS11775 - 1-acyl-sn-glycerol-3-phosphate acyltransferase, at 2,592,002 to 2,592,754 _RS11775 RALBFv3_RS11780: RALBFv3_RS11780 - D-glycero-beta-D-manno-heptose-1,7-bisphosphate 7-phosphatase, at 2,592,751 to 2,593,350 _RS11780 RALBFv3_RS11785: RALBFv3_RS11785 - glycine--tRNA ligase subunit beta, at 2,593,371 to 2,595,464 _RS11785 Position (kb) 2592 2593Strain fitness (log2 ratio) -3 -2 -1 0 1at 2591.155 kb on + strandat 2591.155 kb on + strandat 2591.156 kb on - strandat 2591.221 kb on + strandat 2591.222 kb on + strandat 2591.240 kb on + strand, within RALBFv3_RS11770at 2591.240 kb on + strand, within RALBFv3_RS11770at 2591.241 kb on - strand, within RALBFv3_RS11770at 2591.241 kb on - strand, within RALBFv3_RS11770at 2591.284 kb on + strand, within RALBFv3_RS11770at 2591.285 kb on - strand, within RALBFv3_RS11770at 2591.285 kb on - strand, within RALBFv3_RS11770at 2591.379 kb on + strand, within RALBFv3_RS11770at 2591.379 kb on + strand, within RALBFv3_RS11770at 2591.380 kb on - strandat 2591.386 kb on + strand, within RALBFv3_RS11770at 2591.387 kb on - strand, within RALBFv3_RS11770at 2591.818 kb on + strand, within RALBFv3_RS11770at 2592.171 kb on - strand, within RALBFv3_RS11775at 2592.246 kb on + strand, within RALBFv3_RS11775at 2592.246 kb on + strand, within RALBFv3_RS11775at 2592.450 kb on + strand, within RALBFv3_RS11775at 2592.450 kb on + strand, within RALBFv3_RS11775at 2592.450 kb on + strand, within RALBFv3_RS11775at 2592.450 kb on + strand, within RALBFv3_RS11775at 2592.450 kb on + strand, within RALBFv3_RS11775at 2592.451 kb on - strand, within RALBFv3_RS11775at 2592.480 kb on + strand, within RALBFv3_RS11775at 2592.480 kb on + strand, within RALBFv3_RS11775at 2592.480 kb on + strand, within RALBFv3_RS11775at 2592.481 kb on - strand, within RALBFv3_RS11775at 2592.708 kb on + strandat 2592.709 kb on - strandat 2593.122 kb on - strand, within RALBFv3_RS11780at 2593.339 kb on + strandat 2593.339 kb on + strandat 2593.340 kb on - strandat 2593.372 kb on + strandat 2593.372 kb on + strandat 2593.373 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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2,591,155 + -0.4
2,591,155 + -0.0
2,591,156 - -2.0
2,591,221 + +0.0
2,591,222 + +0.3
2,591,240 + RALBFv3_RS11770 0.12 -0.7
2,591,240 + RALBFv3_RS11770 0.12 -0.3
2,591,241 - RALBFv3_RS11770 0.12 +0.0
2,591,241 - RALBFv3_RS11770 0.12 -0.2
2,591,284 + RALBFv3_RS11770 0.17 +0.2
2,591,285 - RALBFv3_RS11770 0.17 -0.5
2,591,285 - RALBFv3_RS11770 0.17 -0.2
2,591,379 + RALBFv3_RS11770 0.28 +1.3
2,591,379 + RALBFv3_RS11770 0.28 +0.0
2,591,380 - +0.3
2,591,386 + RALBFv3_RS11770 0.28 -0.5
2,591,387 - RALBFv3_RS11770 0.29 +1.0
2,591,818 + RALBFv3_RS11770 0.78 -0.5
2,592,171 - RALBFv3_RS11775 0.22 -1.7
2,592,246 + RALBFv3_RS11775 0.32 -1.3
2,592,246 + RALBFv3_RS11775 0.32 -1.8
2,592,450 + RALBFv3_RS11775 0.59 -0.5
2,592,450 + RALBFv3_RS11775 0.59 -1.3
2,592,450 + RALBFv3_RS11775 0.59 -1.2
2,592,450 + RALBFv3_RS11775 0.59 -0.8
2,592,450 + RALBFv3_RS11775 0.59 -0.8
2,592,451 - RALBFv3_RS11775 0.60 +0.0
2,592,480 + RALBFv3_RS11775 0.63 -1.8
2,592,480 + RALBFv3_RS11775 0.63 +1.0
2,592,480 + RALBFv3_RS11775 0.63 +1.0
2,592,481 - RALBFv3_RS11775 0.64 -1.0
2,592,708 + -1.2
2,592,709 - -0.5
2,593,122 - RALBFv3_RS11780 0.62 -2.9
2,593,339 + -1.5
2,593,339 + -0.5
2,593,340 - -3.6
2,593,372 + -1.8
2,593,372 + -0.8
2,593,373 - -0.3

Or see this region's nucleotide sequence