Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS23950 and RALBFv3_RS11770 are separated by 48 nucleotides RALBFv3_RS11770 and RALBFv3_RS11775 overlap by 4 nucleotides RALBFv3_RS11775 and RALBFv3_RS11780 overlap by 4 nucleotides RALBFv3_RS11780 and RALBFv3_RS11785 are separated by 20 nucleotides
RALBFv3_RS23950: RALBFv3_RS23950 - hypothetical protein, at 2,590,893 to 2,591,090
_RS23950
RALBFv3_RS11770: RALBFv3_RS11770 - DUF45 domain-containing protein, at 2,591,139 to 2,592,005
_RS11770
RALBFv3_RS11775: RALBFv3_RS11775 - 1-acyl-sn-glycerol-3-phosphate acyltransferase, at 2,592,002 to 2,592,754
_RS11775
RALBFv3_RS11780: RALBFv3_RS11780 - D-glycero-beta-D-manno-heptose-1,7-bisphosphate 7-phosphatase, at 2,592,751 to 2,593,350
_RS11780
RALBFv3_RS11785: RALBFv3_RS11785 - glycine--tRNA ligase subunit beta, at 2,593,371 to 2,595,464
_RS11785
Position (kb)
2592
2593 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2591.155 kb on + strand at 2591.155 kb on + strand at 2591.156 kb on - strand at 2591.221 kb on + strand at 2591.222 kb on + strand at 2591.240 kb on + strand, within RALBFv3_RS11770 at 2591.240 kb on + strand, within RALBFv3_RS11770 at 2591.241 kb on - strand, within RALBFv3_RS11770 at 2591.241 kb on - strand, within RALBFv3_RS11770 at 2591.284 kb on + strand, within RALBFv3_RS11770 at 2591.285 kb on - strand, within RALBFv3_RS11770 at 2591.285 kb on - strand, within RALBFv3_RS11770 at 2591.379 kb on + strand, within RALBFv3_RS11770 at 2591.379 kb on + strand, within RALBFv3_RS11770 at 2591.380 kb on - strand at 2591.386 kb on + strand, within RALBFv3_RS11770 at 2591.387 kb on - strand, within RALBFv3_RS11770 at 2591.818 kb on + strand, within RALBFv3_RS11770 at 2592.171 kb on - strand, within RALBFv3_RS11775 at 2592.246 kb on + strand, within RALBFv3_RS11775 at 2592.246 kb on + strand, within RALBFv3_RS11775 at 2592.450 kb on + strand, within RALBFv3_RS11775 at 2592.450 kb on + strand, within RALBFv3_RS11775 at 2592.450 kb on + strand, within RALBFv3_RS11775 at 2592.450 kb on + strand, within RALBFv3_RS11775 at 2592.450 kb on + strand, within RALBFv3_RS11775 at 2592.451 kb on - strand, within RALBFv3_RS11775 at 2592.480 kb on + strand, within RALBFv3_RS11775 at 2592.480 kb on + strand, within RALBFv3_RS11775 at 2592.480 kb on + strand, within RALBFv3_RS11775 at 2592.481 kb on - strand, within RALBFv3_RS11775 at 2592.708 kb on + strand at 2592.709 kb on - strand at 2593.122 kb on - strand, within RALBFv3_RS11780 at 2593.339 kb on + strand at 2593.339 kb on + strand at 2593.340 kb on - strand at 2593.372 kb on + strand at 2593.372 kb on + strand at 2593.373 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 2,591,155 + -0.4 2,591,155 + -0.0 2,591,156 - -2.0 2,591,221 + +0.0 2,591,222 + +0.3 2,591,240 + RALBFv3_RS11770 0.12 -0.7 2,591,240 + RALBFv3_RS11770 0.12 -0.3 2,591,241 - RALBFv3_RS11770 0.12 +0.0 2,591,241 - RALBFv3_RS11770 0.12 -0.2 2,591,284 + RALBFv3_RS11770 0.17 +0.2 2,591,285 - RALBFv3_RS11770 0.17 -0.5 2,591,285 - RALBFv3_RS11770 0.17 -0.2 2,591,379 + RALBFv3_RS11770 0.28 +1.3 2,591,379 + RALBFv3_RS11770 0.28 +0.0 2,591,380 - +0.3 2,591,386 + RALBFv3_RS11770 0.28 -0.5 2,591,387 - RALBFv3_RS11770 0.29 +1.0 2,591,818 + RALBFv3_RS11770 0.78 -0.5 2,592,171 - RALBFv3_RS11775 0.22 -1.7 2,592,246 + RALBFv3_RS11775 0.32 -1.3 2,592,246 + RALBFv3_RS11775 0.32 -1.8 2,592,450 + RALBFv3_RS11775 0.59 -0.5 2,592,450 + RALBFv3_RS11775 0.59 -1.3 2,592,450 + RALBFv3_RS11775 0.59 -1.2 2,592,450 + RALBFv3_RS11775 0.59 -0.8 2,592,450 + RALBFv3_RS11775 0.59 -0.8 2,592,451 - RALBFv3_RS11775 0.60 +0.0 2,592,480 + RALBFv3_RS11775 0.63 -1.8 2,592,480 + RALBFv3_RS11775 0.63 +1.0 2,592,480 + RALBFv3_RS11775 0.63 +1.0 2,592,481 - RALBFv3_RS11775 0.64 -1.0 2,592,708 + -1.2 2,592,709 - -0.5 2,593,122 - RALBFv3_RS11780 0.62 -2.9 2,593,339 + -1.5 2,593,339 + -0.5 2,593,340 - -3.6 2,593,372 + -1.8 2,593,372 + -0.8 2,593,373 - -0.3
Or see this region's nucleotide sequence