Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS11640 and RALBFv3_RS11645 overlap by 4 nucleotides RALBFv3_RS11645 and RALBFv3_RS11650 are separated by 16 nucleotides RALBFv3_RS11650 and RALBFv3_RS11655 overlap by 1 nucleotides
RALBFv3_RS11640: RALBFv3_RS11640 - tyrosine--tRNA ligase, at 2,563,307 to 2,564,578
_RS11640
RALBFv3_RS11645: RALBFv3_RS11645 - D-tyrosyl-tRNA(Tyr) deacylase, at 2,564,575 to 2,565,075
_RS11645
RALBFv3_RS11650: RALBFv3_RS11650 - YbhB/YbcL family Raf kinase inhibitor-like protein, at 2,565,092 to 2,565,727
_RS11650
RALBFv3_RS11655: RALBFv3_RS11655 - histidine phosphatase family protein, at 2,565,727 to 2,566,407
_RS11655
Position (kb)
2564
2565
2566 Strain fitness (log2 ratio)
-1
0
1 at 2564.728 kb on + strand, within RALBFv3_RS11645 at 2565.362 kb on - strand, within RALBFv3_RS11650 at 2565.515 kb on + strand, within RALBFv3_RS11650 at 2565.516 kb on - strand, within RALBFv3_RS11650 at 2565.518 kb on + strand, within RALBFv3_RS11650 at 2565.518 kb on + strand, within RALBFv3_RS11650 at 2565.518 kb on + strand, within RALBFv3_RS11650 at 2565.525 kb on - strand, within RALBFv3_RS11650 at 2565.569 kb on + strand, within RALBFv3_RS11650 at 2565.570 kb on - strand, within RALBFv3_RS11650 at 2565.572 kb on - strand, within RALBFv3_RS11650 at 2565.572 kb on - strand, within RALBFv3_RS11650 at 2565.692 kb on + strand at 2565.693 kb on - strand at 2565.693 kb on - strand at 2565.919 kb on + strand, within RALBFv3_RS11655 at 2565.920 kb on - strand, within RALBFv3_RS11655 at 2566.018 kb on + strand, within RALBFv3_RS11655 at 2566.043 kb on - strand, within RALBFv3_RS11655
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 2,564,728 + RALBFv3_RS11645 0.31 -1.2 2,565,362 - RALBFv3_RS11650 0.42 +0.1 2,565,515 + RALBFv3_RS11650 0.67 +0.2 2,565,516 - RALBFv3_RS11650 0.67 +0.6 2,565,518 + RALBFv3_RS11650 0.67 -0.6 2,565,518 + RALBFv3_RS11650 0.67 -0.4 2,565,518 + RALBFv3_RS11650 0.67 -0.6 2,565,525 - RALBFv3_RS11650 0.68 +0.0 2,565,569 + RALBFv3_RS11650 0.75 -0.5 2,565,570 - RALBFv3_RS11650 0.75 -0.4 2,565,572 - RALBFv3_RS11650 0.75 +1.1 2,565,572 - RALBFv3_RS11650 0.75 -0.5 2,565,692 + -0.3 2,565,693 - +0.8 2,565,693 - +0.4 2,565,919 + RALBFv3_RS11655 0.28 +0.4 2,565,920 - RALBFv3_RS11655 0.28 +0.1 2,566,018 + RALBFv3_RS11655 0.43 +0.3 2,566,043 - RALBFv3_RS11655 0.46 -0.2
Or see this region's nucleotide sequence