Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS11455 and RALBFv3_RS11460 are separated by 17 nucleotides RALBFv3_RS11460 and RALBFv3_RS11465 are separated by 257 nucleotides RALBFv3_RS11465 and RALBFv3_RS11470 are separated by 234 nucleotides RALBFv3_RS11470 and RALBFv3_RS11475 are separated by 28 nucleotides
RALBFv3_RS11455: RALBFv3_RS11455 - ubiquinone biosynthesis regulatory protein kinase UbiB, at 2,524,390 to 2,525,967
_RS11455
RALBFv3_RS11460: RALBFv3_RS11460 - SAM-dependent methyltransferase, at 2,525,985 to 2,526,614
_RS11460
RALBFv3_RS11465: RALBFv3_RS11465 - sodium:solute symporter, at 2,526,872 to 2,528,311
_RS11465
RALBFv3_RS11470: RALBFv3_RS11470 - FmdB family transcriptional regulator, at 2,528,546 to 2,528,887
_RS11470
RALBFv3_RS11475: RALBFv3_RS11475 - DUF502 domain-containing protein, at 2,528,916 to 2,529,653
_RS11475
Position (kb)
2526
2527
2528
2529 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2526.060 kb on + strand, within RALBFv3_RS11460 at 2526.061 kb on - strand, within RALBFv3_RS11460 at 2526.793 kb on + strand at 2526.793 kb on + strand at 2526.793 kb on + strand at 2526.795 kb on + strand at 2526.842 kb on + strand at 2526.842 kb on + strand at 2526.856 kb on - strand at 2526.856 kb on - strand at 2526.892 kb on + strand at 2526.989 kb on + strand at 2526.989 kb on + strand at 2526.990 kb on - strand at 2526.990 kb on - strand at 2527.184 kb on + strand, within RALBFv3_RS11465 at 2527.244 kb on + strand, within RALBFv3_RS11465 at 2527.292 kb on + strand, within RALBFv3_RS11465 at 2527.293 kb on - strand, within RALBFv3_RS11465 at 2527.361 kb on + strand, within RALBFv3_RS11465 at 2527.363 kb on + strand, within RALBFv3_RS11465 at 2527.439 kb on + strand, within RALBFv3_RS11465 at 2527.440 kb on - strand, within RALBFv3_RS11465 at 2527.440 kb on - strand, within RALBFv3_RS11465 at 2527.440 kb on - strand, within RALBFv3_RS11465 at 2527.754 kb on + strand, within RALBFv3_RS11465 at 2528.051 kb on + strand, within RALBFv3_RS11465 at 2528.555 kb on + strand at 2528.555 kb on + strand at 2528.555 kb on + strand at 2528.555 kb on + strand at 2528.705 kb on + strand, within RALBFv3_RS11470
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 2,526,060 + RALBFv3_RS11460 0.12 +0.2 2,526,061 - RALBFv3_RS11460 0.12 +0.5 2,526,793 + +2.1 2,526,793 + +0.7 2,526,793 + +2.1 2,526,795 + +0.1 2,526,842 + +0.7 2,526,842 + -0.0 2,526,856 - -0.9 2,526,856 - -1.0 2,526,892 + -1.0 2,526,989 + -1.9 2,526,989 + -1.3 2,526,990 - -0.5 2,526,990 - -0.6 2,527,184 + RALBFv3_RS11465 0.22 -1.2 2,527,244 + RALBFv3_RS11465 0.26 -2.2 2,527,292 + RALBFv3_RS11465 0.29 -0.7 2,527,293 - RALBFv3_RS11465 0.29 -1.2 2,527,361 + RALBFv3_RS11465 0.34 -0.3 2,527,363 + RALBFv3_RS11465 0.34 -1.1 2,527,439 + RALBFv3_RS11465 0.39 -1.0 2,527,440 - RALBFv3_RS11465 0.39 -0.9 2,527,440 - RALBFv3_RS11465 0.39 -1.8 2,527,440 - RALBFv3_RS11465 0.39 -1.1 2,527,754 + RALBFv3_RS11465 0.61 -0.8 2,528,051 + RALBFv3_RS11465 0.82 +0.1 2,528,555 + -0.1 2,528,555 + -0.3 2,528,555 + +0.3 2,528,555 + +0.3 2,528,705 + RALBFv3_RS11470 0.46 +0.5
Or see this region's nucleotide sequence