Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS11465

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS11455 and RALBFv3_RS11460 are separated by 17 nucleotidesRALBFv3_RS11460 and RALBFv3_RS11465 are separated by 257 nucleotidesRALBFv3_RS11465 and RALBFv3_RS11470 are separated by 234 nucleotidesRALBFv3_RS11470 and RALBFv3_RS11475 are separated by 28 nucleotides RALBFv3_RS11455: RALBFv3_RS11455 - ubiquinone biosynthesis regulatory protein kinase UbiB, at 2,524,390 to 2,525,967 _RS11455 RALBFv3_RS11460: RALBFv3_RS11460 - SAM-dependent methyltransferase, at 2,525,985 to 2,526,614 _RS11460 RALBFv3_RS11465: RALBFv3_RS11465 - sodium:solute symporter, at 2,526,872 to 2,528,311 _RS11465 RALBFv3_RS11470: RALBFv3_RS11470 - FmdB family transcriptional regulator, at 2,528,546 to 2,528,887 _RS11470 RALBFv3_RS11475: RALBFv3_RS11475 - DUF502 domain-containing protein, at 2,528,916 to 2,529,653 _RS11475 Position (kb) 2526 2527 2528 2529Strain fitness (log2 ratio) -2 -1 0 1 2at 2526.060 kb on + strand, within RALBFv3_RS11460at 2526.061 kb on - strand, within RALBFv3_RS11460at 2526.793 kb on + strandat 2526.793 kb on + strandat 2526.793 kb on + strandat 2526.795 kb on + strandat 2526.842 kb on + strandat 2526.842 kb on + strandat 2526.856 kb on - strandat 2526.856 kb on - strandat 2526.892 kb on + strandat 2526.989 kb on + strandat 2526.989 kb on + strandat 2526.990 kb on - strandat 2526.990 kb on - strandat 2527.184 kb on + strand, within RALBFv3_RS11465at 2527.244 kb on + strand, within RALBFv3_RS11465at 2527.292 kb on + strand, within RALBFv3_RS11465at 2527.293 kb on - strand, within RALBFv3_RS11465at 2527.361 kb on + strand, within RALBFv3_RS11465at 2527.363 kb on + strand, within RALBFv3_RS11465at 2527.439 kb on + strand, within RALBFv3_RS11465at 2527.440 kb on - strand, within RALBFv3_RS11465at 2527.440 kb on - strand, within RALBFv3_RS11465at 2527.440 kb on - strand, within RALBFv3_RS11465at 2527.754 kb on + strand, within RALBFv3_RS11465at 2528.051 kb on + strand, within RALBFv3_RS11465at 2528.555 kb on + strandat 2528.555 kb on + strandat 2528.555 kb on + strandat 2528.555 kb on + strandat 2528.705 kb on + strand, within RALBFv3_RS11470

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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2,526,060 + RALBFv3_RS11460 0.12 +0.2
2,526,061 - RALBFv3_RS11460 0.12 +0.5
2,526,793 + +2.1
2,526,793 + +0.7
2,526,793 + +2.1
2,526,795 + +0.1
2,526,842 + +0.7
2,526,842 + -0.0
2,526,856 - -0.9
2,526,856 - -1.0
2,526,892 + -1.0
2,526,989 + -1.9
2,526,989 + -1.3
2,526,990 - -0.5
2,526,990 - -0.6
2,527,184 + RALBFv3_RS11465 0.22 -1.2
2,527,244 + RALBFv3_RS11465 0.26 -2.2
2,527,292 + RALBFv3_RS11465 0.29 -0.7
2,527,293 - RALBFv3_RS11465 0.29 -1.2
2,527,361 + RALBFv3_RS11465 0.34 -0.3
2,527,363 + RALBFv3_RS11465 0.34 -1.1
2,527,439 + RALBFv3_RS11465 0.39 -1.0
2,527,440 - RALBFv3_RS11465 0.39 -0.9
2,527,440 - RALBFv3_RS11465 0.39 -1.8
2,527,440 - RALBFv3_RS11465 0.39 -1.1
2,527,754 + RALBFv3_RS11465 0.61 -0.8
2,528,051 + RALBFv3_RS11465 0.82 +0.1
2,528,555 + -0.1
2,528,555 + -0.3
2,528,555 + +0.3
2,528,555 + +0.3
2,528,705 + RALBFv3_RS11470 0.46 +0.5

Or see this region's nucleotide sequence