Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS11310 and RALBFv3_RS11315 are separated by 67 nucleotides RALBFv3_RS11315 and RALBFv3_RS11320 are separated by 487 nucleotides RALBFv3_RS11320 and RALBFv3_RS11325 are separated by 100 nucleotides
RALBFv3_RS11310: RALBFv3_RS11310 - beta-hydroxyacyl-ACP dehydratase, at 2,492,714 to 2,494,405
_RS11310
RALBFv3_RS11315: RALBFv3_RS11315 - membrane protein, at 2,494,473 to 2,495,129
_RS11315
RALBFv3_RS11320: RALBFv3_RS11320 - acyl carrier protein, at 2,495,617 to 2,495,988
_RS11320
RALBFv3_RS11325: RALBFv3_RS11325 - 3-oxoacyl-ACP reductase FabG, at 2,496,089 to 2,496,805
_RS11325
Position (kb)
2494
2495
2496 Strain fitness (log2 ratio)
-1
0
1 at 2493.579 kb on + strand, within RALBFv3_RS11310 at 2493.580 kb on - strand, within RALBFv3_RS11310 at 2493.781 kb on - strand, within RALBFv3_RS11310 at 2493.781 kb on - strand, within RALBFv3_RS11310 at 2493.831 kb on - strand, within RALBFv3_RS11310 at 2493.831 kb on - strand, within RALBFv3_RS11310 at 2493.849 kb on + strand, within RALBFv3_RS11310 at 2493.850 kb on - strand, within RALBFv3_RS11310 at 2493.850 kb on - strand, within RALBFv3_RS11310 at 2493.850 kb on - strand, within RALBFv3_RS11310 at 2494.003 kb on - strand, within RALBFv3_RS11310 at 2494.395 kb on + strand at 2494.423 kb on + strand at 2494.423 kb on + strand at 2494.730 kb on + strand, within RALBFv3_RS11315 at 2494.731 kb on - strand, within RALBFv3_RS11315 at 2494.864 kb on + strand, within RALBFv3_RS11315 at 2494.865 kb on - strand, within RALBFv3_RS11315 at 2495.258 kb on + strand at 2495.331 kb on - strand at 2495.386 kb on + strand at 2495.386 kb on + strand at 2495.386 kb on + strand at 2495.566 kb on - strand at 2495.586 kb on + strand at 2495.597 kb on + strand at 2495.602 kb on + strand at 2495.602 kb on + strand at 2495.603 kb on - strand at 2495.617 kb on - strand at 2495.787 kb on + strand, within RALBFv3_RS11320 at 2495.788 kb on - strand, within RALBFv3_RS11320 at 2495.822 kb on + strand, within RALBFv3_RS11320 at 2495.823 kb on - strand, within RALBFv3_RS11320 at 2495.823 kb on - strand, within RALBFv3_RS11320 at 2495.823 kb on - strand, within RALBFv3_RS11320 at 2495.823 kb on - strand, within RALBFv3_RS11320 at 2495.824 kb on + strand, within RALBFv3_RS11320 at 2495.986 kb on + strand at 2495.987 kb on - strand at 2495.989 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 2,493,579 + RALBFv3_RS11310 0.51 -1.3 2,493,580 - RALBFv3_RS11310 0.51 +0.1 2,493,781 - RALBFv3_RS11310 0.63 -0.3 2,493,781 - RALBFv3_RS11310 0.63 +0.7 2,493,831 - RALBFv3_RS11310 0.66 -0.2 2,493,831 - RALBFv3_RS11310 0.66 -0.1 2,493,849 + RALBFv3_RS11310 0.67 -0.3 2,493,850 - RALBFv3_RS11310 0.67 +1.1 2,493,850 - RALBFv3_RS11310 0.67 +0.4 2,493,850 - RALBFv3_RS11310 0.67 -0.7 2,494,003 - RALBFv3_RS11310 0.76 +0.2 2,494,395 + -0.4 2,494,423 + -0.7 2,494,423 + -0.3 2,494,730 + RALBFv3_RS11315 0.39 -0.3 2,494,731 - RALBFv3_RS11315 0.39 -1.1 2,494,864 + RALBFv3_RS11315 0.60 -1.1 2,494,865 - RALBFv3_RS11315 0.60 +1.1 2,495,258 + -0.2 2,495,331 - -0.0 2,495,386 + -0.2 2,495,386 + +0.2 2,495,386 + -0.3 2,495,566 - +1.4 2,495,586 + -0.1 2,495,597 + +0.6 2,495,602 + +1.1 2,495,602 + +1.1 2,495,603 - +1.1 2,495,617 - -0.6 2,495,787 + RALBFv3_RS11320 0.46 -0.4 2,495,788 - RALBFv3_RS11320 0.46 -0.4 2,495,822 + RALBFv3_RS11320 0.55 -0.1 2,495,823 - RALBFv3_RS11320 0.55 +0.6 2,495,823 - RALBFv3_RS11320 0.55 +1.1 2,495,823 - RALBFv3_RS11320 0.55 +0.1 2,495,823 - RALBFv3_RS11320 0.55 +1.1 2,495,824 + RALBFv3_RS11320 0.56 -0.1 2,495,986 + +1.0 2,495,987 - -1.4 2,495,989 + -1.2
Or see this region's nucleotide sequence