Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS11315

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS11310 and RALBFv3_RS11315 are separated by 67 nucleotidesRALBFv3_RS11315 and RALBFv3_RS11320 are separated by 487 nucleotidesRALBFv3_RS11320 and RALBFv3_RS11325 are separated by 100 nucleotides RALBFv3_RS11310: RALBFv3_RS11310 - beta-hydroxyacyl-ACP dehydratase, at 2,492,714 to 2,494,405 _RS11310 RALBFv3_RS11315: RALBFv3_RS11315 - membrane protein, at 2,494,473 to 2,495,129 _RS11315 RALBFv3_RS11320: RALBFv3_RS11320 - acyl carrier protein, at 2,495,617 to 2,495,988 _RS11320 RALBFv3_RS11325: RALBFv3_RS11325 - 3-oxoacyl-ACP reductase FabG, at 2,496,089 to 2,496,805 _RS11325 Position (kb) 2494 2495 2496Strain fitness (log2 ratio) -1 0 1at 2493.579 kb on + strand, within RALBFv3_RS11310at 2493.580 kb on - strand, within RALBFv3_RS11310at 2493.781 kb on - strand, within RALBFv3_RS11310at 2493.781 kb on - strand, within RALBFv3_RS11310at 2493.831 kb on - strand, within RALBFv3_RS11310at 2493.831 kb on - strand, within RALBFv3_RS11310at 2493.849 kb on + strand, within RALBFv3_RS11310at 2493.850 kb on - strand, within RALBFv3_RS11310at 2493.850 kb on - strand, within RALBFv3_RS11310at 2493.850 kb on - strand, within RALBFv3_RS11310at 2494.003 kb on - strand, within RALBFv3_RS11310at 2494.395 kb on + strandat 2494.423 kb on + strandat 2494.423 kb on + strandat 2494.730 kb on + strand, within RALBFv3_RS11315at 2494.731 kb on - strand, within RALBFv3_RS11315at 2494.864 kb on + strand, within RALBFv3_RS11315at 2494.865 kb on - strand, within RALBFv3_RS11315at 2495.258 kb on + strandat 2495.331 kb on - strandat 2495.386 kb on + strandat 2495.386 kb on + strandat 2495.386 kb on + strandat 2495.566 kb on - strandat 2495.586 kb on + strandat 2495.597 kb on + strandat 2495.602 kb on + strandat 2495.602 kb on + strandat 2495.603 kb on - strandat 2495.617 kb on - strandat 2495.787 kb on + strand, within RALBFv3_RS11320at 2495.788 kb on - strand, within RALBFv3_RS11320at 2495.822 kb on + strand, within RALBFv3_RS11320at 2495.823 kb on - strand, within RALBFv3_RS11320at 2495.823 kb on - strand, within RALBFv3_RS11320at 2495.823 kb on - strand, within RALBFv3_RS11320at 2495.823 kb on - strand, within RALBFv3_RS11320at 2495.824 kb on + strand, within RALBFv3_RS11320at 2495.986 kb on + strandat 2495.987 kb on - strandat 2495.989 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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2,493,579 + RALBFv3_RS11310 0.51 -1.3
2,493,580 - RALBFv3_RS11310 0.51 +0.1
2,493,781 - RALBFv3_RS11310 0.63 -0.3
2,493,781 - RALBFv3_RS11310 0.63 +0.7
2,493,831 - RALBFv3_RS11310 0.66 -0.2
2,493,831 - RALBFv3_RS11310 0.66 -0.1
2,493,849 + RALBFv3_RS11310 0.67 -0.3
2,493,850 - RALBFv3_RS11310 0.67 +1.1
2,493,850 - RALBFv3_RS11310 0.67 +0.4
2,493,850 - RALBFv3_RS11310 0.67 -0.7
2,494,003 - RALBFv3_RS11310 0.76 +0.2
2,494,395 + -0.4
2,494,423 + -0.7
2,494,423 + -0.3
2,494,730 + RALBFv3_RS11315 0.39 -0.3
2,494,731 - RALBFv3_RS11315 0.39 -1.1
2,494,864 + RALBFv3_RS11315 0.60 -1.1
2,494,865 - RALBFv3_RS11315 0.60 +1.1
2,495,258 + -0.2
2,495,331 - -0.0
2,495,386 + -0.2
2,495,386 + +0.2
2,495,386 + -0.3
2,495,566 - +1.4
2,495,586 + -0.1
2,495,597 + +0.6
2,495,602 + +1.1
2,495,602 + +1.1
2,495,603 - +1.1
2,495,617 - -0.6
2,495,787 + RALBFv3_RS11320 0.46 -0.4
2,495,788 - RALBFv3_RS11320 0.46 -0.4
2,495,822 + RALBFv3_RS11320 0.55 -0.1
2,495,823 - RALBFv3_RS11320 0.55 +0.6
2,495,823 - RALBFv3_RS11320 0.55 +1.1
2,495,823 - RALBFv3_RS11320 0.55 +0.1
2,495,823 - RALBFv3_RS11320 0.55 +1.1
2,495,824 + RALBFv3_RS11320 0.56 -0.1
2,495,986 + +1.0
2,495,987 - -1.4
2,495,989 + -1.2

Or see this region's nucleotide sequence