Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS11135 and RALBFv3_RS11140 are separated by 29 nucleotides RALBFv3_RS11140 and RALBFv3_RS11145 are separated by 77 nucleotides RALBFv3_RS11145 and RALBFv3_RS11150 are separated by 93 nucleotides RALBFv3_RS11150 and RALBFv3_RS11155 are separated by 190 nucleotides
RALBFv3_RS11135: RALBFv3_RS11135 - A/G-specific adenine glycosylase, at 2,457,691 to 2,458,839
_RS11135
RALBFv3_RS11140: RALBFv3_RS11140 - ATP-dependent protease, at 2,458,869 to 2,459,522
_RS11140
RALBFv3_RS11145: RALBFv3_RS11145 - RNase adapter RapZ, at 2,459,600 to 2,460,490
_RS11145
RALBFv3_RS11150: RALBFv3_RS11150 - hypothetical protein, at 2,460,584 to 2,460,916
_RS11150
RALBFv3_RS11155: RALBFv3_RS11155 - HPr kinase/phosphorylase, at 2,461,107 to 2,462,081
_RS11155
Position (kb)
2459
2460
2461 Strain fitness (log2 ratio)
-2
-1
0
1 at 2459.021 kb on - strand, within RALBFv3_RS11140 at 2459.021 kb on - strand, within RALBFv3_RS11140 at 2459.021 kb on - strand, within RALBFv3_RS11140 at 2459.392 kb on + strand, within RALBFv3_RS11140 at 2459.392 kb on + strand, within RALBFv3_RS11140 at 2459.512 kb on + strand at 2459.602 kb on - strand at 2459.610 kb on - strand at 2459.641 kb on + strand at 2459.658 kb on + strand at 2459.683 kb on - strand at 2459.683 kb on - strand at 2459.730 kb on + strand, within RALBFv3_RS11145 at 2459.775 kb on + strand, within RALBFv3_RS11145 at 2459.775 kb on + strand, within RALBFv3_RS11145 at 2459.776 kb on - strand, within RALBFv3_RS11145 at 2459.878 kb on - strand, within RALBFv3_RS11145 at 2460.006 kb on + strand, within RALBFv3_RS11145 at 2460.006 kb on + strand, within RALBFv3_RS11145 at 2460.007 kb on - strand, within RALBFv3_RS11145 at 2460.290 kb on + strand, within RALBFv3_RS11145 at 2460.346 kb on - strand, within RALBFv3_RS11145 at 2460.363 kb on + strand, within RALBFv3_RS11145 at 2460.412 kb on - strand at 2460.501 kb on + strand at 2460.745 kb on - strand, within RALBFv3_RS11150
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 2,459,021 - RALBFv3_RS11140 0.23 -0.1 2,459,021 - RALBFv3_RS11140 0.23 -0.1 2,459,021 - RALBFv3_RS11140 0.23 -0.1 2,459,392 + RALBFv3_RS11140 0.80 -0.1 2,459,392 + RALBFv3_RS11140 0.80 +0.1 2,459,512 + -1.5 2,459,602 - -0.0 2,459,610 - -1.0 2,459,641 + +0.1 2,459,658 + -1.2 2,459,683 - +1.1 2,459,683 - -1.7 2,459,730 + RALBFv3_RS11145 0.15 +0.1 2,459,775 + RALBFv3_RS11145 0.20 +1.1 2,459,775 + RALBFv3_RS11145 0.20 -0.5 2,459,776 - RALBFv3_RS11145 0.20 -2.5 2,459,878 - RALBFv3_RS11145 0.31 +0.1 2,460,006 + RALBFv3_RS11145 0.46 +0.1 2,460,006 + RALBFv3_RS11145 0.46 -1.7 2,460,007 - RALBFv3_RS11145 0.46 -0.5 2,460,290 + RALBFv3_RS11145 0.77 -2.5 2,460,346 - RALBFv3_RS11145 0.84 -1.2 2,460,363 + RALBFv3_RS11145 0.86 +1.1 2,460,412 - +0.5 2,460,501 + -0.1 2,460,745 - RALBFv3_RS11150 0.48 +0.2
Or see this region's nucleotide sequence