Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS11125 and RALBFv3_RS11130 are separated by 263 nucleotides RALBFv3_RS11130 and RALBFv3_RS11135 are separated by 116 nucleotides
RALBFv3_RS11125: RALBFv3_RS11125 - bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase, at 2,454,501 to 2,455,367
_RS11125
RALBFv3_RS11130: RALBFv3_RS11130 - GTPase, at 2,455,631 to 2,457,574
_RS11130
RALBFv3_RS11135: RALBFv3_RS11135 - A/G-specific adenine glycosylase, at 2,457,691 to 2,458,839
_RS11135
Position (kb)
2455
2456
2457
2458 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2454.676 kb on - strand, within RALBFv3_RS11125 at 2454.886 kb on + strand, within RALBFv3_RS11125 at 2454.988 kb on - strand, within RALBFv3_RS11125 at 2454.988 kb on - strand, within RALBFv3_RS11125 at 2455.065 kb on - strand, within RALBFv3_RS11125 at 2455.065 kb on - strand, within RALBFv3_RS11125 at 2455.068 kb on + strand, within RALBFv3_RS11125 at 2455.069 kb on - strand, within RALBFv3_RS11125 at 2455.069 kb on - strand, within RALBFv3_RS11125 at 2455.069 kb on - strand, within RALBFv3_RS11125 at 2455.069 kb on - strand, within RALBFv3_RS11125 at 2455.344 kb on + strand at 2455.344 kb on + strand at 2455.619 kb on + strand at 2455.619 kb on + strand at 2455.620 kb on - strand at 2455.664 kb on + strand at 2455.665 kb on - strand at 2455.685 kb on + strand at 2455.687 kb on - strand at 2455.736 kb on + strand at 2455.932 kb on - strand, within RALBFv3_RS11130 at 2455.932 kb on - strand, within RALBFv3_RS11130 at 2455.944 kb on - strand, within RALBFv3_RS11130 at 2456.138 kb on + strand, within RALBFv3_RS11130 at 2456.138 kb on + strand, within RALBFv3_RS11130 at 2456.141 kb on - strand, within RALBFv3_RS11130 at 2457.107 kb on + strand, within RALBFv3_RS11130 at 2457.107 kb on + strand, within RALBFv3_RS11130 at 2457.107 kb on + strand, within RALBFv3_RS11130 at 2457.685 kb on - strand at 2457.685 kb on - strand at 2457.685 kb on - strand at 2457.991 kb on + strand, within RALBFv3_RS11135 at 2457.994 kb on + strand, within RALBFv3_RS11135 at 2458.288 kb on + strand, within RALBFv3_RS11135 at 2458.289 kb on - strand, within RALBFv3_RS11135 at 2458.326 kb on + strand, within RALBFv3_RS11135 at 2458.522 kb on - strand, within RALBFv3_RS11135
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 2,454,676 - RALBFv3_RS11125 0.20 -0.9 2,454,886 + RALBFv3_RS11125 0.44 +1.1 2,454,988 - RALBFv3_RS11125 0.56 -0.9 2,454,988 - RALBFv3_RS11125 0.56 +1.1 2,455,065 - RALBFv3_RS11125 0.65 -0.1 2,455,065 - RALBFv3_RS11125 0.65 -0.2 2,455,068 + RALBFv3_RS11125 0.65 -0.4 2,455,069 - RALBFv3_RS11125 0.66 +0.0 2,455,069 - RALBFv3_RS11125 0.66 -0.4 2,455,069 - RALBFv3_RS11125 0.66 +0.2 2,455,069 - RALBFv3_RS11125 0.66 -1.3 2,455,344 + -0.2 2,455,344 + -0.4 2,455,619 + -0.1 2,455,619 + -0.7 2,455,620 - -1.1 2,455,664 + -1.9 2,455,665 - -0.1 2,455,685 + -1.6 2,455,687 - -1.9 2,455,736 + +0.1 2,455,932 - RALBFv3_RS11130 0.15 -2.3 2,455,932 - RALBFv3_RS11130 0.15 -1.9 2,455,944 - RALBFv3_RS11130 0.16 -2.3 2,456,138 + RALBFv3_RS11130 0.26 -2.1 2,456,138 + RALBFv3_RS11130 0.26 -3.2 2,456,141 - RALBFv3_RS11130 0.26 -1.9 2,457,107 + RALBFv3_RS11130 0.76 -1.4 2,457,107 + RALBFv3_RS11130 0.76 -2.8 2,457,107 + RALBFv3_RS11130 0.76 -2.3 2,457,685 - -0.3 2,457,685 - -0.5 2,457,685 - -0.0 2,457,991 + RALBFv3_RS11135 0.26 -0.3 2,457,994 + RALBFv3_RS11135 0.26 +1.0 2,458,288 + RALBFv3_RS11135 0.52 -0.2 2,458,289 - RALBFv3_RS11135 0.52 -0.4 2,458,326 + RALBFv3_RS11135 0.55 -0.2 2,458,522 - RALBFv3_RS11135 0.72 +0.1
Or see this region's nucleotide sequence