Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS11130

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS11125 and RALBFv3_RS11130 are separated by 263 nucleotidesRALBFv3_RS11130 and RALBFv3_RS11135 are separated by 116 nucleotides RALBFv3_RS11125: RALBFv3_RS11125 - bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase, at 2,454,501 to 2,455,367 _RS11125 RALBFv3_RS11130: RALBFv3_RS11130 - GTPase, at 2,455,631 to 2,457,574 _RS11130 RALBFv3_RS11135: RALBFv3_RS11135 - A/G-specific adenine glycosylase, at 2,457,691 to 2,458,839 _RS11135 Position (kb) 2455 2456 2457 2458Strain fitness (log2 ratio) -3 -2 -1 0 1at 2454.676 kb on - strand, within RALBFv3_RS11125at 2454.886 kb on + strand, within RALBFv3_RS11125at 2454.988 kb on - strand, within RALBFv3_RS11125at 2454.988 kb on - strand, within RALBFv3_RS11125at 2455.065 kb on - strand, within RALBFv3_RS11125at 2455.065 kb on - strand, within RALBFv3_RS11125at 2455.068 kb on + strand, within RALBFv3_RS11125at 2455.069 kb on - strand, within RALBFv3_RS11125at 2455.069 kb on - strand, within RALBFv3_RS11125at 2455.069 kb on - strand, within RALBFv3_RS11125at 2455.069 kb on - strand, within RALBFv3_RS11125at 2455.344 kb on + strandat 2455.344 kb on + strandat 2455.619 kb on + strandat 2455.619 kb on + strandat 2455.620 kb on - strandat 2455.664 kb on + strandat 2455.665 kb on - strandat 2455.685 kb on + strandat 2455.687 kb on - strandat 2455.736 kb on + strandat 2455.932 kb on - strand, within RALBFv3_RS11130at 2455.932 kb on - strand, within RALBFv3_RS11130at 2455.944 kb on - strand, within RALBFv3_RS11130at 2456.138 kb on + strand, within RALBFv3_RS11130at 2456.138 kb on + strand, within RALBFv3_RS11130at 2456.141 kb on - strand, within RALBFv3_RS11130at 2457.107 kb on + strand, within RALBFv3_RS11130at 2457.107 kb on + strand, within RALBFv3_RS11130at 2457.107 kb on + strand, within RALBFv3_RS11130at 2457.685 kb on - strandat 2457.685 kb on - strandat 2457.685 kb on - strandat 2457.991 kb on + strand, within RALBFv3_RS11135at 2457.994 kb on + strand, within RALBFv3_RS11135at 2458.288 kb on + strand, within RALBFv3_RS11135at 2458.289 kb on - strand, within RALBFv3_RS11135at 2458.326 kb on + strand, within RALBFv3_RS11135at 2458.522 kb on - strand, within RALBFv3_RS11135

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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2,454,676 - RALBFv3_RS11125 0.20 -0.9
2,454,886 + RALBFv3_RS11125 0.44 +1.1
2,454,988 - RALBFv3_RS11125 0.56 -0.9
2,454,988 - RALBFv3_RS11125 0.56 +1.1
2,455,065 - RALBFv3_RS11125 0.65 -0.1
2,455,065 - RALBFv3_RS11125 0.65 -0.2
2,455,068 + RALBFv3_RS11125 0.65 -0.4
2,455,069 - RALBFv3_RS11125 0.66 +0.0
2,455,069 - RALBFv3_RS11125 0.66 -0.4
2,455,069 - RALBFv3_RS11125 0.66 +0.2
2,455,069 - RALBFv3_RS11125 0.66 -1.3
2,455,344 + -0.2
2,455,344 + -0.4
2,455,619 + -0.1
2,455,619 + -0.7
2,455,620 - -1.1
2,455,664 + -1.9
2,455,665 - -0.1
2,455,685 + -1.6
2,455,687 - -1.9
2,455,736 + +0.1
2,455,932 - RALBFv3_RS11130 0.15 -2.3
2,455,932 - RALBFv3_RS11130 0.15 -1.9
2,455,944 - RALBFv3_RS11130 0.16 -2.3
2,456,138 + RALBFv3_RS11130 0.26 -2.1
2,456,138 + RALBFv3_RS11130 0.26 -3.2
2,456,141 - RALBFv3_RS11130 0.26 -1.9
2,457,107 + RALBFv3_RS11130 0.76 -1.4
2,457,107 + RALBFv3_RS11130 0.76 -2.8
2,457,107 + RALBFv3_RS11130 0.76 -2.3
2,457,685 - -0.3
2,457,685 - -0.5
2,457,685 - -0.0
2,457,991 + RALBFv3_RS11135 0.26 -0.3
2,457,994 + RALBFv3_RS11135 0.26 +1.0
2,458,288 + RALBFv3_RS11135 0.52 -0.2
2,458,289 - RALBFv3_RS11135 0.52 -0.4
2,458,326 + RALBFv3_RS11135 0.55 -0.2
2,458,522 - RALBFv3_RS11135 0.72 +0.1

Or see this region's nucleotide sequence