Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS11055 and RALBFv3_RS11060 are separated by 60 nucleotides RALBFv3_RS11060 and RALBFv3_RS11065 overlap by 1 nucleotides
RALBFv3_RS11055: RALBFv3_RS11055 - signal recognition particle-docking protein FtsY, at 2,441,727 to 2,442,860
_RS11055
RALBFv3_RS11060: RALBFv3_RS11060 - insulinase family protein, at 2,442,921 to 2,444,414
_RS11060
RALBFv3_RS11065: RALBFv3_RS11065 - insulinase family protein, at 2,444,414 to 2,445,757
_RS11065
Position (kb)
2442
2443
2444
2445 Strain fitness (log2 ratio)
-2
-1
0
1 at 2443.182 kb on + strand, within RALBFv3_RS11060 at 2443.513 kb on - strand, within RALBFv3_RS11060 at 2443.620 kb on + strand, within RALBFv3_RS11060 at 2443.620 kb on + strand, within RALBFv3_RS11060 at 2443.710 kb on + strand, within RALBFv3_RS11060 at 2443.710 kb on + strand, within RALBFv3_RS11060 at 2443.710 kb on + strand, within RALBFv3_RS11060 at 2444.160 kb on + strand, within RALBFv3_RS11060 at 2444.160 kb on + strand, within RALBFv3_RS11060 at 2444.161 kb on - strand, within RALBFv3_RS11060 at 2444.161 kb on - strand, within RALBFv3_RS11060 at 2444.302 kb on - strand at 2444.957 kb on + strand, within RALBFv3_RS11065 at 2444.958 kb on - strand, within RALBFv3_RS11065 at 2445.071 kb on - strand, within RALBFv3_RS11065 at 2445.302 kb on + strand, within RALBFv3_RS11065 at 2445.371 kb on + strand, within RALBFv3_RS11065 at 2445.371 kb on + strand, within RALBFv3_RS11065 at 2445.372 kb on - strand, within RALBFv3_RS11065 at 2445.372 kb on - strand, within RALBFv3_RS11065 at 2445.372 kb on - strand, within RALBFv3_RS11065 at 2445.373 kb on + strand, within RALBFv3_RS11065 at 2445.374 kb on - strand, within RALBFv3_RS11065 at 2445.374 kb on - strand, within RALBFv3_RS11065
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 2,443,182 + RALBFv3_RS11060 0.17 -1.0 2,443,513 - RALBFv3_RS11060 0.40 -0.6 2,443,620 + RALBFv3_RS11060 0.47 -1.1 2,443,620 + RALBFv3_RS11060 0.47 -2.1 2,443,710 + RALBFv3_RS11060 0.53 -0.7 2,443,710 + RALBFv3_RS11060 0.53 -0.6 2,443,710 + RALBFv3_RS11060 0.53 +1.1 2,444,160 + RALBFv3_RS11060 0.83 -0.9 2,444,160 + RALBFv3_RS11060 0.83 -0.3 2,444,161 - RALBFv3_RS11060 0.83 -0.8 2,444,161 - RALBFv3_RS11060 0.83 +0.1 2,444,302 - +1.1 2,444,957 + RALBFv3_RS11065 0.40 +1.0 2,444,958 - RALBFv3_RS11065 0.40 +0.8 2,445,071 - RALBFv3_RS11065 0.49 -0.2 2,445,302 + RALBFv3_RS11065 0.66 -0.3 2,445,371 + RALBFv3_RS11065 0.71 -0.9 2,445,371 + RALBFv3_RS11065 0.71 -0.9 2,445,372 - RALBFv3_RS11065 0.71 +1.1 2,445,372 - RALBFv3_RS11065 0.71 +1.1 2,445,372 - RALBFv3_RS11065 0.71 -0.8 2,445,373 + RALBFv3_RS11065 0.71 -0.2 2,445,374 - RALBFv3_RS11065 0.71 -0.2 2,445,374 - RALBFv3_RS11065 0.71 -0.8
Or see this region's nucleotide sequence